FastQCFastQC Report
Thu 26 May 2016
SRR1546994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546994_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2511243
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109040.4342072830068615No Hit
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT29490.117431885325315TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT27890.1110605385460507TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC27160.10815361157801137TruSeq Adapter, Index 27 (96% over 25bp)
GAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT25250.10054781636026462TruSeq Adapter, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69500.040.3057561
GACACGA10450.039.83253525
CACGACC10450.039.83253527
TCAAGCG10600.039.69339817
CCGAATA403.460409E-739.37528
TTACGGG6500.039.1153873
TAGGGAC16450.038.8449865
CGTAAGG3650.038.8356172
GCGTAAG1450.038.7931021
AACACGT10000.038.69999741
GGGCGAT58600.038.6646777
TGGGCGA15550.038.6334426
CGCGATA356.251248E-638.57142634
AGACACG11000.038.45454424
TATGGGC11500.038.3478244
TTTACGG3350.038.2835852
ACACGAC10900.038.18807226
ACGACCA10850.038.1566828
TATAGCG1300.038.076921
GGCGATT16150.037.8947378
Produced by FastQC (version 0.11.5)