##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546994_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2511243
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551889641902438	31.0	31.0	33.0	30.0	34.0
2	31.919969911314833	31.0	31.0	34.0	30.0	34.0
3	32.032463206467874	33.0	31.0	34.0	30.0	34.0
4	35.708651054477805	37.0	35.0	37.0	33.0	37.0
5	35.65402432181991	37.0	35.0	37.0	33.0	37.0
6	35.70674920746419	37.0	35.0	37.0	33.0	37.0
7	36.06411207517552	37.0	35.0	37.0	35.0	37.0
8	35.98556292640736	37.0	35.0	37.0	35.0	37.0
9	37.74336772665966	39.0	38.0	39.0	35.0	39.0
10	37.255366764586306	39.0	37.0	39.0	34.0	39.0
11	37.01071262319098	39.0	37.0	39.0	33.0	39.0
12	36.49645175715771	39.0	35.0	39.0	32.0	39.0
13	36.31312979269629	39.0	35.0	39.0	32.0	39.0
14	37.333325767359035	40.0	35.0	41.0	32.0	41.0
15	37.46178486112256	40.0	35.0	41.0	32.0	41.0
16	37.5759741291464	40.0	35.0	41.0	33.0	41.0
17	37.523445162415584	39.0	35.0	41.0	33.0	41.0
18	37.45639111786474	39.0	35.0	41.0	32.0	41.0
19	37.4022541824905	39.0	35.0	41.0	32.0	41.0
20	37.26574927237229	39.0	35.0	41.0	32.0	41.0
21	37.18736299115617	39.0	35.0	41.0	32.0	41.0
22	37.10552742207744	39.0	35.0	41.0	32.0	41.0
23	37.02822745548718	39.0	35.0	41.0	32.0	41.0
24	36.905191970669506	39.0	35.0	41.0	32.0	41.0
25	36.79809998474859	38.0	35.0	41.0	31.0	41.0
26	36.646798019944704	38.0	35.0	41.0	31.0	41.0
27	36.566399587773866	38.0	35.0	41.0	31.0	41.0
28	36.59564486590904	38.0	35.0	41.0	31.0	41.0
29	36.60143323445799	38.0	35.0	41.0	31.0	41.0
30	36.516074708819495	38.0	35.0	41.0	31.0	41.0
31	36.39710135578277	38.0	35.0	41.0	31.0	41.0
32	36.29480500294078	38.0	35.0	41.0	30.0	41.0
33	36.184468408672515	38.0	35.0	41.0	30.0	41.0
34	36.02837797855484	38.0	35.0	41.0	30.0	41.0
35	35.92969935605595	38.0	35.0	41.0	30.0	41.0
36	35.837937626904285	38.0	35.0	41.0	29.0	41.0
37	35.756611367358715	38.0	35.0	41.0	29.0	41.0
38	35.65142919263488	38.0	35.0	41.0	29.0	41.0
39	35.546457272354765	38.0	35.0	40.0	28.0	41.0
40	35.37730797059464	38.0	35.0	40.0	27.0	41.0
41	35.2975773352081	38.0	34.0	40.0	27.0	41.0
42	35.19535903136415	37.0	34.0	40.0	27.0	41.0
43	35.057948991794106	37.0	34.0	40.0	26.0	41.0
44	34.905265241157466	37.0	34.0	40.0	26.0	41.0
45	34.782169228545385	37.0	34.0	40.0	26.0	41.0
46	34.73262165389809	36.0	34.0	40.0	25.0	41.0
47	34.67208191321987	36.0	34.0	40.0	25.0	41.0
48	34.56670501421009	36.0	34.0	40.0	25.0	41.0
49	34.50688603213628	36.0	34.0	40.0	24.0	41.0
50	34.36847409828519	36.0	34.0	40.0	24.0	41.0
51	33.98763719799319	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	11.0
13	8.0
14	38.0
15	65.0
16	241.0
17	566.0
18	1111.0
19	2144.0
20	3558.0
21	5714.0
22	8720.0
23	12928.0
24	18909.0
25	25913.0
26	33500.0
27	39342.0
28	44393.0
29	50047.0
30	58933.0
31	70036.0
32	86222.0
33	112030.0
34	190859.0
35	299541.0
36	186384.0
37	239046.0
38	353059.0
39	667413.0
40	507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.21945108458242	24.51642473468318	27.287761479076302	12.976362701658104
2	31.7096354275552	25.675372713831358	28.63386776986536	13.981124088748082
3	30.73943859674273	25.024181252073173	30.371453499322847	13.86492665186125
4	26.03622986704194	28.910065652746468	29.088343899813758	15.965360580397835
5	23.89987747103725	32.77034520355059	28.214792435459252	15.114984889952904
6	21.77897559097228	41.69672150405198	25.474237260193455	11.050065644782284
7	87.51108514787298	3.705416003150631	6.842348589921405	1.941150259054978
8	86.41720454770805	3.3457534774611615	6.587693823337686	3.649348151493105
9	80.77107631559352	5.156530053045444	10.00106321849379	4.071330412867254
10	44.49716733904286	23.31411177651864	16.342146100556576	15.846574783881925
11	36.356656842846355	24.945654403018743	22.29039563276035	16.407293121374554
12	32.03815799586101	21.593011906852503	26.746316465590947	19.62251363169554
13	23.836681675170425	29.487588417369402	28.885774893150522	17.789955014309648
14	20.161131360047595	31.338783224084647	29.729659774064075	18.77042564180368
15	19.056618574944757	24.05740902015456	38.88504617036265	18.000926234538035
16	21.92571567148221	21.39848672549809	36.465009559011214	20.210788044008485
17	22.013919003457648	22.73929683427689	27.967902747762764	27.2788814145027
18	24.247872467937192	23.173822684622714	31.37565739356964	21.202647453870455
19	26.968198617178825	26.174448271234603	25.607796617053786	21.249556494532786
20	27.895110110809668	25.46918000368742	25.134166625850224	21.50154325965269
21	25.10676983469939	27.131185631975878	28.041412161228525	19.72063237209621
22	24.31198414490354	23.32175739265376	26.76240411620859	25.60385434623412
23	23.071841315237116	27.704686483944407	25.482958041097575	23.740514159720902
24	22.862861140877246	24.70652979420948	32.18330523967613	20.247303825237143
25	22.61278577979112	24.687017544697984	29.317513279280423	23.382683396230473
26	20.460425375003535	28.542478764500288	27.340205627253116	23.656890233243058
27	21.914406530949016	27.230459178980286	27.36393889400588	23.491195396064818
28	19.601129799067632	27.27398344166614	30.451533364154727	22.673353395111505
29	20.97435413458594	25.526880512957128	29.36075879554468	24.138006556912252
30	22.787480144295078	26.15712617217848	28.521413499211345	22.533980184315098
31	25.878260287833555	26.171461702431824	25.091677707016007	22.858600302718614
32	26.659108656549762	25.16128467057947	24.98105519856103	23.198551474309735
33	24.70672889879633	25.41773137844486	25.32319652060752	24.552343202151285
34	20.357368840848935	25.985298913725195	28.880160143801298	24.777172101624572
35	20.602386945429014	25.768035988552285	29.182201802055797	24.447375263962908
36	25.70444198351175	25.22543616846319	26.907033688097886	22.163088159927174
37	21.84647204591511	27.449195478095906	27.46110193238966	23.243230543599324
38	22.25567179281336	27.628628531766935	26.806008020729177	23.309691654690525
39	22.06883204851143	25.893870087442757	27.129791899867918	24.907505964177897
40	24.178623892630064	23.339278596296733	28.421821384867975	24.06027612620523
41	19.76347967918676	23.599269365808087	28.124000743854737	28.513250211150414
42	23.02668439493908	25.161961626174765	26.321984770091944	25.48936920879421
43	22.034028566729702	23.840783229659575	28.31948162722604	25.805706576384686
44	22.3954033918661	25.607756796136417	27.24137011033978	24.755469701657706
45	20.67474155229104	26.89735720517688	26.421457421683208	26.00644382084888
46	23.221329039045603	25.698389204071447	27.800893820311295	23.27938793657165
47	22.146044807292643	24.86493740350894	28.565136866484046	24.423880922714368
48	22.225129149190263	24.14254614149248	29.633771005036152	23.998553704281107
49	22.870466936094992	22.730934441629106	29.31755310019779	25.08104552207811
50	21.328800120099885	25.895781491476534	28.561672446672826	24.21374594175076
51	20.764776646465517	26.32286083027409	26.99276812319636	25.91959440006403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1669.0
1	2641.5
2	3614.0
3	11245.5
4	18877.0
5	13497.0
6	8117.0
7	8475.5
8	8834.0
9	9439.5
10	10045.0
11	10485.5
12	10926.0
13	11051.5
14	11177.0
15	11291.0
16	11405.0
17	10894.5
18	10384.0
19	10615.5
20	10847.0
21	10794.0
22	10741.0
23	11768.5
24	12796.0
25	12809.5
26	14785.5
27	16748.0
28	18827.5
29	20907.0
30	24757.5
31	28608.0
32	34255.5
33	39903.0
34	44114.0
35	48325.0
36	54202.5
37	60080.0
38	70274.0
39	80468.0
40	99084.0
41	117700.0
42	134746.5
43	151793.0
44	167603.5
45	183414.0
46	198623.5
47	213833.0
48	222065.0
49	230297.0
50	220213.0
51	210129.0
52	190392.5
53	170656.0
54	157837.0
55	145018.0
56	137263.5
57	129509.0
58	120137.0
59	110765.0
60	105081.0
61	99397.0
62	92910.5
63	86424.0
64	77021.0
65	67618.0
66	58246.5
67	48875.0
68	41817.5
69	34760.0
70	30628.5
71	26497.0
72	22122.5
73	17748.0
74	14922.0
75	10420.5
76	8745.0
77	6853.5
78	4962.0
79	3374.0
80	1786.0
81	1438.5
82	1091.0
83	782.5
84	474.0
85	325.5
86	177.0
87	132.0
88	87.0
89	84.0
90	81.0
91	46.0
92	11.0
93	6.5
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2511243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.810383977387033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65265477092505	17.15444601394738
2	8.104453723209227	3.535224952603145
3	2.8131494539648765	1.840676093302519
4	1.4241777454609936	1.242474539222146
5	0.8746155302328911	0.9537850273482655
6	0.5823489932553769	0.7620753091046727
7	0.43872666112186537	0.669815785812939
8	0.3522139680737288	0.614553750870973
9	0.28778213608338993	0.5648975000830244
>10	3.837089452073993	20.80844450163627
>50	1.9756103769722568	31.599505066622136
>100	0.6507016504704828	18.104601085769236
>500	0.003330276765888757	0.5110248691944536
>1k	0.00296024601412334	1.1984711477475736
>5k	0.0	0.0
>10k+	1.8501537588270874E-4	0.44000435673533816
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10904	0.4342072830068615	No Hit
GAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2949	0.117431885325315	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2789	0.1110605385460507	TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2716	0.10815361157801137	TruSeq Adapter, Index 27 (96% over 25bp)
GAGAGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	2525	0.10054781636026462	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.964183474080365E-5	0.0	0.0	0.12567481522098817	0.0
2	7.964183474080365E-5	0.0	0.0	0.43213659530360066	0.0
3	7.964183474080365E-5	0.0	0.0	0.6172242192412284	0.0
4	7.964183474080365E-5	0.0	0.0	1.0911329568663806	0.0
5	7.964183474080365E-5	0.0	0.0	1.7649426996909499	0.0
6	7.964183474080365E-5	0.0	0.0	2.8559960147225896	0.0
7	7.964183474080365E-5	0.0	0.0	3.335320397110116	0.0
8	7.964183474080365E-5	0.0	0.0	4.658330555824347	0.0
9	7.964183474080365E-5	0.0	0.0	5.166604745140155	0.0
10	7.964183474080365E-5	0.0	0.0	6.019090944205718	0.0
11	7.964183474080365E-5	0.0	0.0	6.796076683936999	0.0
12	7.964183474080365E-5	0.0	0.0	7.402111225397144	0.0
13	7.964183474080365E-5	0.0	0.0	7.686830784595517	0.0
14	1.1946275211120548E-4	0.0	0.0	7.816169124214582	0.0
15	1.1946275211120548E-4	0.0	0.0	7.96864341682585	0.0
16	1.1946275211120548E-4	0.0	0.0	8.280281916166615	0.0
17	1.1946275211120548E-4	0.0	0.0	8.616848309781252	0.0
18	1.1946275211120548E-4	0.0	0.0	9.077138293665726	0.0
19	1.1946275211120548E-4	0.0	0.0	9.297746175897753	0.0
20	1.1946275211120548E-4	0.0	0.0	9.536711501037534	0.0
21	1.1946275211120548E-4	0.0	0.0	9.828877571784172	0.0
22	1.1946275211120548E-4	0.0	0.0	10.130441379030225	0.0
23	1.1946275211120548E-4	0.0	0.0	10.475609090796869	0.0
24	1.1946275211120548E-4	0.0	0.0	10.732533649670701	0.0
25	1.592836694816073E-4	0.0	0.0	10.951389411538429	0.0
26	1.592836694816073E-4	0.0	0.0	11.162719020023152	0.0
27	1.592836694816073E-4	0.0	0.0	11.375442360615839	0.0
28	1.592836694816073E-4	0.0	0.0	11.592307076615047	0.0
29	1.592836694816073E-4	0.0	0.0	11.833582014962312	0.0
30	1.592836694816073E-4	0.0	0.0	12.149959203470154	0.0
31	1.592836694816073E-4	0.0	0.0	12.404852895558097	0.0
32	1.592836694816073E-4	0.0	0.0	12.648795835369178	0.0
33	1.592836694816073E-4	0.0	0.0	12.900304749480636	0.0
34	1.592836694816073E-4	0.0	0.0	13.144725540300161	0.0
35	1.592836694816073E-4	0.0	0.0	13.43087865252387	0.0
36	1.592836694816073E-4	0.0	0.0	13.675817115269211	0.0
37	1.592836694816073E-4	0.0	0.0	13.925573909016371	0.0
38	2.3892550422241097E-4	0.0	0.0	14.211408453901116	0.0
39	2.3892550422241097E-4	0.0	0.0	14.61017512044832	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6950	0.0	40.305756	1
GACACGA	1045	0.0	39.832535	25
CACGACC	1045	0.0	39.832535	27
TCAAGCG	1060	0.0	39.693398	17
CCGAATA	40	3.460409E-7	39.375	28
TTACGGG	650	0.0	39.115387	3
TAGGGAC	1645	0.0	38.844986	5
CGTAAGG	365	0.0	38.835617	2
GCGTAAG	145	0.0	38.793102	1
AACACGT	1000	0.0	38.699997	41
GGGCGAT	5860	0.0	38.664677	7
TGGGCGA	1555	0.0	38.633442	6
CGCGATA	35	6.251248E-6	38.571426	34
AGACACG	1100	0.0	38.454544	24
TATGGGC	1150	0.0	38.347824	4
TTTACGG	335	0.0	38.283585	2
ACACGAC	1090	0.0	38.188072	26
ACGACCA	1085	0.0	38.15668	28
TATAGCG	130	0.0	38.07692	1
GGCGATT	1615	0.0	37.894737	8
>>END_MODULE