FastQCFastQC Report
Thu 26 May 2016
SRR1546993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546993_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1099446
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.5219901659563089No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG253.890694E-545.01
CGTTTTT32350.039.7140661
TAGTAGG5500.039.681822
GGGCGAT23450.039.4349677
CTAACGG800.039.3750042
TCGTAAG403.4580444E-739.3750041
TTAATCG1950.039.2307720
TTACGGG1950.039.230773
GAGGGTA2900.038.7931025
ACTACGG700.038.5714262
CGGTCTA700.038.57142631
ATAGGGC5750.037.956524
CATATGC4750.037.89473733
CACGACC4050.037.7777827
AGCATAT4650.037.74193631
CGAGGGA4950.037.7272724
AACGGGA2150.037.674424
GGCGATA4600.037.6630448
TATGGGC4250.037.5882384
TAAGGGA9050.037.5414354
Produced by FastQC (version 0.11.5)