##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546993_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1099446
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611588927514404	31.0	31.0	33.0	30.0	34.0
2	31.992173330932125	31.0	31.0	34.0	30.0	34.0
3	32.123497652454056	33.0	31.0	34.0	30.0	34.0
4	35.80218491858627	37.0	35.0	37.0	35.0	37.0
5	35.747131737256765	37.0	35.0	37.0	33.0	37.0
6	35.793563303700225	37.0	35.0	37.0	35.0	37.0
7	36.09160249798535	37.0	35.0	37.0	35.0	37.0
8	36.00961484238425	37.0	35.0	37.0	35.0	37.0
9	37.78136443263244	39.0	38.0	39.0	35.0	39.0
10	37.3375581883967	39.0	37.0	39.0	34.0	39.0
11	37.0800585021911	39.0	37.0	39.0	33.0	39.0
12	36.592508408780425	39.0	35.0	39.0	33.0	39.0
13	36.42442011704076	39.0	35.0	39.0	32.0	39.0
14	37.52941026662519	40.0	35.0	41.0	33.0	41.0
15	37.63745922946648	40.0	35.0	41.0	33.0	41.0
16	37.72787749466549	40.0	35.0	41.0	33.0	41.0
17	37.67904017114074	40.0	35.0	41.0	33.0	41.0
18	37.60735679605911	39.0	36.0	41.0	33.0	41.0
19	37.56274523714671	39.0	35.0	41.0	33.0	41.0
20	37.43043314542051	39.0	35.0	41.0	33.0	41.0
21	37.36285274583745	39.0	35.0	41.0	33.0	41.0
22	37.253060177580345	39.0	35.0	41.0	32.0	41.0
23	37.1696472587103	39.0	35.0	41.0	32.0	41.0
24	37.0541809238471	39.0	35.0	41.0	32.0	41.0
25	36.92759353347049	39.0	35.0	41.0	32.0	41.0
26	36.78063497434162	38.0	35.0	41.0	31.0	41.0
27	36.67781500864981	38.0	35.0	41.0	31.0	41.0
28	36.69281983835495	38.0	35.0	41.0	31.0	41.0
29	36.67920298041013	38.0	35.0	41.0	31.0	41.0
30	36.613458960239974	38.0	35.0	41.0	31.0	41.0
31	36.52126889360642	38.0	35.0	41.0	31.0	41.0
32	36.418809109315056	38.0	35.0	41.0	31.0	41.0
33	36.2986404061682	38.0	35.0	41.0	30.0	41.0
34	36.146883066562616	38.0	35.0	41.0	30.0	41.0
35	36.04327543144456	38.0	35.0	41.0	30.0	41.0
36	35.933001711771205	38.0	35.0	41.0	30.0	41.0
37	35.83274121694017	38.0	35.0	41.0	29.0	41.0
38	35.74013275777073	38.0	35.0	41.0	29.0	41.0
39	35.633158881836856	38.0	35.0	40.0	29.0	41.0
40	35.48420750086862	38.0	35.0	40.0	28.0	41.0
41	35.40592443830802	37.0	35.0	40.0	28.0	41.0
42	35.30791871542577	37.0	35.0	40.0	28.0	41.0
43	35.1840472383364	37.0	34.0	40.0	27.0	41.0
44	35.04187017825341	37.0	34.0	40.0	27.0	41.0
45	34.92191067137449	37.0	34.0	40.0	26.0	41.0
46	34.86669649987357	36.0	34.0	40.0	26.0	41.0
47	34.79725880125081	36.0	34.0	40.0	26.0	41.0
48	34.70773644180797	36.0	34.0	40.0	26.0	41.0
49	34.61211737547819	36.0	34.0	40.0	26.0	41.0
50	34.4943680726475	35.0	34.0	40.0	26.0	41.0
51	34.128543830256326	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	2.0
14	7.0
15	28.0
16	74.0
17	218.0
18	480.0
19	887.0
20	1453.0
21	2322.0
22	3458.0
23	5180.0
24	7463.0
25	10472.0
26	13570.0
27	16498.0
28	18322.0
29	20935.0
30	24167.0
31	28793.0
32	35464.0
33	46407.0
34	82559.0
35	134483.0
36	83615.0
37	109257.0
38	157931.0
39	295130.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.08412418618104	24.285049015595128	27.675483834585783	12.95534296363805
2	31.37616581441926	26.569017486988898	28.577301659199268	13.477515039392568
3	30.29653116205798	25.729776632958778	29.77736059797389	14.196331607009348
4	26.586117008020405	28.657796744906072	29.23617894830669	15.519907298766833
5	23.845918762722317	33.39008919037406	27.872401191145357	14.891590855758263
6	22.00808407143234	42.58462898587106	25.053708867920754	10.353578074775841
7	88.08290720963103	4.004562297739043	6.1681064827194785	1.7444240099104458
8	87.60766786181404	3.627736150752288	6.111532535476958	2.6530634519567124
9	82.38767524735184	5.358516925797174	8.664636553318672	3.5891712735323065
10	47.420428106519104	23.998086308922858	14.347134829723334	14.234350754834708
11	36.76187825504845	25.256083518426554	21.176938203422452	16.805100023102547
12	32.53393072511065	21.260707665496987	26.554009928636784	19.65135168075558
13	23.776338264907963	29.10011041924751	28.016382796426566	19.10716851941796
14	21.10863107419555	30.858359573821726	28.504719649714495	19.528289702268236
15	19.986975258448343	23.352670344882785	37.35872430296713	19.30163009370174
16	22.074844967374478	20.433563813047662	36.459907990024064	21.031683229553792
17	21.995532295356025	21.377948530441696	27.778353825472102	28.848165348730177
18	22.940007967649162	22.939553193153642	30.648890441185834	23.471548398011365
19	27.341315535278675	24.628494714610813	25.41725559963836	22.612934150472146
20	29.438189779216078	23.206596776922193	25.277366964816828	22.0778464790449
21	26.148987762927874	25.57469852998692	26.189553647928136	22.086760059157065
22	25.78780585858696	23.410972435208276	24.364270732714477	26.436950973490287
23	24.47287088224433	26.747016224534903	24.638317843714017	24.14179504950675
24	24.510799075170585	23.49947155203621	30.99642911066119	20.99330026213202
25	23.85938008778967	24.13206287530265	28.470429652752387	23.538127384155295
26	22.4630404767492	27.881951455551253	26.13634503195246	23.518663035747096
27	23.038784988075815	27.12611624399925	25.953798549451268	23.881300218473665
28	21.569863367550564	26.831422370903162	29.736612803175415	21.86210145837085
29	22.518250100505163	24.549272997491464	28.603041895645624	24.32943500635775
30	23.823634812441902	26.643327639556652	27.201426900457136	22.33161064754431
31	27.595534478273603	25.86939240308301	23.241796322875338	23.29327679576805
32	28.29452287788577	25.08827172958017	24.10250253309394	22.51470285944012
33	26.82914849842557	26.557920989298246	23.20623295732578	23.406697554950405
34	23.61607573268719	26.4958897481095	25.660378044942632	24.227656474260673
35	23.970526974494426	25.61881165605223	26.439679620463398	23.970981748989946
36	28.552834791340366	26.967218035265034	23.099997635172624	21.379949538221975
37	24.46313870804023	29.190428634057515	24.72154157639393	21.624891081508324
38	24.86397694839037	28.495624159804116	23.99108278169187	22.64931611011364
39	24.076034657454752	28.23458359937641	24.443947224329342	23.245434518839488
40	26.032201672478685	25.869756222679424	24.85197090170868	23.246071203133216
41	21.64008054965865	24.965391660891033	26.429492671763775	26.965035117686543
42	24.10423067617691	25.933242742253825	24.470778919564946	25.491747662004315
43	24.377459193084515	24.389556194665314	26.666975913323622	24.56600869892655
44	23.503291657798563	26.09978116251276	25.7497867107616	24.647140468927077
45	22.18189888361957	27.616363150168354	24.883350341899465	25.31838762431261
46	24.105958819259882	26.991502993325728	25.70312684752139	23.199411339893
47	23.744958824717173	25.62326844610831	27.084549855108847	23.547222874065664
48	24.67670081113579	23.66846575457094	27.65101696672688	24.00381646756639
49	23.71558039230667	24.36872752277056	27.481295124999317	24.43439695992345
50	22.71989711181813	26.423307738624725	27.22562090361873	23.631174245938407
51	21.929499038606718	27.273281270749088	25.43162647369675	25.365593216947445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	680.0
1	1163.0
2	1646.0
3	5523.0
4	9400.0
5	6573.0
6	3746.0
7	3830.0
8	3914.0
9	4109.5
10	4305.0
11	4513.5
12	4722.0
13	4766.5
14	4811.0
15	4737.0
16	4663.0
17	4646.5
18	4630.0
19	4498.5
20	4367.0
21	4671.0
22	4975.0
23	4762.5
24	4550.0
25	5253.0
26	6357.0
27	6758.0
28	8329.0
29	9900.0
30	11995.0
31	14090.0
32	15499.5
33	16909.0
34	18385.5
35	19862.0
36	22087.5
37	24313.0
38	28212.5
39	32112.0
40	39448.0
41	46784.0
42	54171.0
43	61558.0
44	66361.0
45	71164.0
46	76232.0
47	81300.0
48	81924.0
49	82548.0
50	79749.0
51	76950.0
52	73381.5
53	69813.0
54	66955.5
55	64098.0
56	62427.0
57	60756.0
58	60106.5
59	59457.0
60	57506.0
61	55555.0
62	50677.5
63	45800.0
64	41797.5
65	37795.0
66	33550.5
67	29306.0
68	25920.0
69	22534.0
70	19567.5
71	16601.0
72	14733.5
73	12866.0
74	10509.5
75	6692.0
76	5231.0
77	3927.5
78	2624.0
79	1895.0
80	1166.0
81	838.5
82	511.0
83	398.5
84	286.0
85	233.0
86	180.0
87	125.0
88	70.0
89	43.5
90	17.0
91	14.0
92	11.0
93	7.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1099446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.837206617372605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06549685469065	18.370261715957398
2	6.102509833221938	2.9093357555811874
3	2.216284753984621	1.5849011281099268
4	1.230739382719107	1.1734955583205189
5	0.947792986457062	1.129636862433681
6	0.7718742064440214	1.1039594964975992
7	0.6307401065551268	1.052455756927272
8	0.569529512962622	1.0860794140145287
9	0.48884150820832056	1.0487354430878806
>10	8.97785364743121	54.05577981318211
>50	0.9479976502022317	13.605285238732012
>100	0.0468811142672871	1.8723868995764947
>500	0.0030741714273630804	0.4819969603038745
>1k	0.0	0.0
>5k	3.8427142842038505E-4	0.5256899572755485
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5739	0.5219901659563089	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.095489910373043E-5	0.0	0.0	0.06385033917081875	0.0
2	9.095489910373043E-5	0.0	0.0	0.2011922368174517	0.0
3	9.095489910373043E-5	0.0	0.0	0.2982411141611321	0.0
4	9.095489910373043E-5	0.0	0.0	0.5426369280528557	0.0
5	9.095489910373043E-5	0.0	0.0	0.8741675352859531	0.0
6	9.095489910373043E-5	0.0	0.0	1.4935703981823574	0.0
7	9.095489910373043E-5	0.0	0.0	1.7654345916034075	0.0
8	9.095489910373043E-5	0.0	0.0	2.565291974321613	0.0
9	9.095489910373043E-5	0.0	0.0	2.9146497417790416	0.0
10	9.095489910373043E-5	0.0	0.0	3.4747500104598132	0.0
11	9.095489910373043E-5	0.0	0.0	3.9663612401154764	0.0
12	9.095489910373043E-5	0.0	0.0	4.365744202079957	0.0
13	9.095489910373043E-5	0.0	0.0	4.556112805904064	0.0
14	9.095489910373043E-5	0.0	0.0	4.640518952272326	0.0
15	9.095489910373043E-5	0.0	0.0	4.746936184223691	9.095489910373043E-5
16	9.095489910373043E-5	0.0	0.0	4.952039481702603	9.095489910373043E-5
17	9.095489910373043E-5	0.0	0.0	5.178517180470892	9.095489910373043E-5
18	9.095489910373043E-5	0.0	0.0	5.491947762782346	9.095489910373043E-5
19	9.095489910373043E-5	0.0	0.0	5.634474089677893	9.095489910373043E-5
20	9.095489910373043E-5	0.0	0.0	5.793190388613902	9.095489910373043E-5
21	9.095489910373043E-5	0.0	0.0	5.993836896036731	9.095489910373043E-5
22	9.095489910373043E-5	0.0	0.0	6.198667328818332	9.095489910373043E-5
23	9.095489910373043E-5	0.0	0.0	6.424417388393791	9.095489910373043E-5
24	9.095489910373043E-5	0.0	0.0	6.610420157060919	9.095489910373043E-5
25	9.095489910373043E-5	0.0	0.0	6.761860064068631	9.095489910373043E-5
26	9.095489910373043E-5	0.0	0.0	6.902021563587479	9.095489910373043E-5
27	9.095489910373043E-5	0.0	0.0	7.040000145527839	9.095489910373043E-5
28	9.095489910373043E-5	0.0	0.0	7.184527480203666	9.095489910373043E-5
29	9.095489910373043E-5	0.0	0.0	7.33796839499166	9.095489910373043E-5
30	9.095489910373043E-5	0.0	0.0	7.540070180800148	9.095489910373043E-5
31	9.095489910373043E-5	0.0	0.0	7.70906438333488	9.095489910373043E-5
32	9.095489910373043E-5	0.0	0.0	7.872237472326972	9.095489910373043E-5
33	9.095489910373043E-5	0.0	0.0	8.052055307855047	9.095489910373043E-5
34	9.095489910373043E-5	0.0	0.0	8.22768921802435	9.095489910373043E-5
35	9.095489910373043E-5	0.0	0.0	8.423060341299163	9.095489910373043E-5
36	9.095489910373043E-5	0.0	0.0	8.603605816020067	9.095489910373043E-5
37	9.095489910373043E-5	0.0	0.0	8.779876410483098	9.095489910373043E-5
38	9.095489910373043E-5	0.0	0.0	8.970063104508998	9.095489910373043E-5
39	9.095489910373043E-5	0.0	0.0	9.155793008478815	9.095489910373043E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	25	3.890694E-5	45.0	1
CGTTTTT	3235	0.0	39.714066	1
TAGTAGG	550	0.0	39.68182	2
GGGCGAT	2345	0.0	39.434967	7
CTAACGG	80	0.0	39.375004	2
TCGTAAG	40	3.4580444E-7	39.375004	1
TTAATCG	195	0.0	39.23077	20
TTACGGG	195	0.0	39.23077	3
GAGGGTA	290	0.0	38.793102	5
ACTACGG	70	0.0	38.571426	2
CGGTCTA	70	0.0	38.571426	31
ATAGGGC	575	0.0	37.95652	4
CATATGC	475	0.0	37.894737	33
CACGACC	405	0.0	37.77778	27
AGCATAT	465	0.0	37.741936	31
CGAGGGA	495	0.0	37.727272	4
AACGGGA	215	0.0	37.67442	4
GGCGATA	460	0.0	37.663044	8
TATGGGC	425	0.0	37.588238	4
TAAGGGA	905	0.0	37.541435	4
>>END_MODULE