FastQCFastQC Report
Thu 26 May 2016
SRR1546992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546992_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2779443
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237700.8552073203156173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC551.8189894E-1245.012
CGTTTTT135000.041.7666631
AGGGCGA36500.039.267126
GCGATAT2650.039.0566029
GGGCGAT70050.038.38337
GGCGATA13850.036.8772548
GACCGAT18650.036.7962469
CACGACC22950.036.76470627
GTTGATC15000.036.7516
ACACGAC22900.036.74672726
TAAGGGA29950.036.5859764
CGTAAGG3450.036.521742
ACACGTG20650.036.3922542
TAGGGAT26850.036.2849165
TAGGGCG14950.036.2709055
TTGATCC15300.036.17646817
GCGATAA3550.036.1267629
CATACGA3800.036.1184218
AGGGAAC31400.036.042996
AGGGATT28600.036.0314676
Produced by FastQC (version 0.11.5)