FastQCFastQC Report
Thu 26 May 2016
SRR1546991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546991_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1598191
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.42097596595150394No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33360.20873600214242224No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT20160.12614261999973722No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT16200.10136460535693168No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTC207.0341147E-445.014
CCGTAAC207.0341147E-445.037
CGTACTT207.0341147E-445.029
ATACGTG207.0341147E-445.01
TGTACCC253.8914914E-545.031
AATACGG650.044.9999962
TAGTACG650.044.9999961
TAATGCG1050.042.8571431
AGGGTAC7450.042.5838936
TGCGTTT900.042.528
ACGTTAG850.042.352941
AACGGGA4550.041.5384644
TAACGAG650.041.538461
CGTTAGG1850.041.3513532
CTCATGC3050.041.31147823
CGTTTTT50000.041.0850031
ACACGAC6150.040.9756126
CGTAAGG1650.040.909092
CACGACC6150.040.60975627
CGCATCG501.0822987E-940.521