##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546991_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1598191
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615722401139788	31.0	31.0	33.0	30.0	34.0
2	31.991481618905375	31.0	31.0	34.0	30.0	34.0
3	32.086722425542376	33.0	31.0	34.0	30.0	34.0
4	35.751112351402305	37.0	35.0	37.0	35.0	37.0
5	35.70076417649705	37.0	35.0	37.0	33.0	37.0
6	35.75340056351212	37.0	35.0	37.0	33.0	37.0
7	36.08890113885011	37.0	35.0	37.0	35.0	37.0
8	36.01743346070651	37.0	35.0	37.0	35.0	37.0
9	37.7836322442061	39.0	38.0	39.0	35.0	39.0
10	37.350839793241235	39.0	37.0	39.0	34.0	39.0
11	37.06281664707159	39.0	37.0	39.0	33.0	39.0
12	36.38141185878283	38.0	35.0	39.0	33.0	39.0
13	36.08407693448405	38.0	35.0	39.0	32.0	39.0
14	37.11069390329441	39.0	35.0	41.0	32.0	41.0
15	37.31524204553774	39.0	35.0	41.0	32.0	41.0
16	37.445557508458	39.0	35.0	41.0	33.0	41.0
17	37.398230874782804	39.0	35.0	41.0	33.0	41.0
18	37.34962654651415	39.0	35.0	41.0	33.0	41.0
19	37.279530419080075	39.0	35.0	41.0	33.0	41.0
20	37.10712236522418	38.0	35.0	41.0	32.0	41.0
21	37.013428933087475	38.0	35.0	41.0	32.0	41.0
22	36.906412312420734	38.0	35.0	41.0	32.0	41.0
23	36.82930763594589	38.0	35.0	41.0	32.0	41.0
24	36.70502461845925	38.0	35.0	41.0	32.0	41.0
25	36.59506529569995	38.0	35.0	41.0	31.0	41.0
26	36.42980594935148	38.0	35.0	40.0	31.0	41.0
27	36.32591786588712	38.0	35.0	40.0	31.0	41.0
28	36.36631416395162	38.0	35.0	40.0	31.0	41.0
29	36.382278463587895	38.0	35.0	40.0	31.0	41.0
30	36.32762792432194	38.0	35.0	40.0	31.0	41.0
31	36.189506761081745	38.0	35.0	40.0	31.0	41.0
32	36.03422932553118	38.0	35.0	40.0	30.0	41.0
33	35.88481602011274	38.0	35.0	40.0	30.0	41.0
34	35.707056290518466	38.0	35.0	40.0	30.0	41.0
35	35.60876703723147	37.0	35.0	40.0	29.0	41.0
36	35.43275928847053	37.0	35.0	40.0	29.0	41.0
37	35.317217403927316	37.0	35.0	40.0	28.0	41.0
38	35.23103058395398	37.0	34.0	40.0	28.0	41.0
39	35.09341999798522	37.0	34.0	40.0	27.0	41.0
40	34.92750616165402	36.0	34.0	40.0	26.0	41.0
41	34.854821482538696	36.0	34.0	40.0	26.0	41.0
42	34.75889677766925	36.0	34.0	40.0	26.0	41.0
43	34.62738621353768	36.0	34.0	40.0	26.0	41.0
44	34.4986087395061	36.0	34.0	40.0	25.0	41.0
45	34.386099658926874	35.0	34.0	40.0	24.0	41.0
46	34.31458192418803	35.0	34.0	40.0	24.0	41.0
47	34.25842780994262	35.0	34.0	40.0	24.0	41.0
48	34.16546144985174	35.0	34.0	40.0	24.0	41.0
49	34.087271170967675	35.0	34.0	40.0	24.0	41.0
50	33.956246155809914	35.0	34.0	39.0	24.0	41.0
51	33.57622524466725	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	7.0
13	6.0
14	15.0
15	56.0
16	157.0
17	353.0
18	848.0
19	1479.0
20	2420.0
21	3841.0
22	5919.0
23	8501.0
24	12594.0
25	17561.0
26	22588.0
27	26323.0
28	29280.0
29	32889.0
30	38268.0
31	45119.0
32	55099.0
33	72260.0
34	131631.0
35	240457.0
36	116194.0
37	145205.0
38	210479.0
39	378301.0
40	337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.56145104058276	24.25461036884828	28.256948011845896	12.92699057872307
2	31.436355229130935	27.11503193297922	28.06397983720344	13.384633000686403
3	30.33073018181181	25.23108940045339	30.55467087475777	13.883509542977029
4	25.881699997059176	28.487521203660886	29.796000603181973	15.834778196097963
5	23.577219493790167	33.441059297668424	28.17110095101274	14.810620257528667
6	22.035538931204094	42.020196584763646	25.453090400333878	10.491174083698382
7	87.2645384688063	4.107894488205727	6.894107149896351	1.7334598930916267
8	86.59959917181364	3.8984076371347354	6.276721618379781	3.225271572671852
9	81.06221346509898	5.855870793916371	9.267665754593787	3.814249986390863
10	49.94534445507452	23.14003770513036	13.804294981012907	13.110322858782212
11	41.6988958140798	23.657748041379286	19.602412978173447	15.040943166367473
12	37.1641437099821	20.397499422784886	24.544438055276245	17.89391881195677
13	23.293273457302664	34.59780464287435	25.951528947416175	16.157392952406816
14	18.477578712431743	36.635358352036775	27.083058282770956	17.804004652760526
15	17.200384684934402	23.80335016277779	42.22974600657869	16.766519145709115
16	18.57037112585417	19.179434748412426	42.29075248202499	19.959441643708416
17	18.904624040555852	20.27229536394586	28.044582906548715	32.77849768894957
18	21.9319843498055	22.4789152235246	31.399438490142916	24.189661936526985
19	28.285229988155358	25.263563616614032	24.42423965596102	22.02696673926959
20	30.957376183447412	23.457709372659462	24.131658856794964	21.453255587098162
21	25.39427390092924	27.31187949375262	25.65951128494654	21.634335320371594
22	24.663385039710523	25.051010799084715	23.38644129518937	26.89916286601539
23	24.188473092390083	29.481770326575486	22.423102119834237	23.906654461200194
24	22.673385096024194	24.29972387530652	33.22931989981173	19.797571128857562
25	21.795580127781975	24.09511754227123	31.004804807435406	23.104497522511387
26	21.3003326886461	30.54897693704945	25.450962995036264	22.699727379268186
27	22.350958051947483	29.37421121755785	25.674653405006037	22.60017732548863
28	20.160544015077047	27.72015359866249	32.58139984519998	19.537902541060486
29	20.242761972755446	24.610325048758252	31.001300845768746	24.145612132717552
30	22.954890873493845	26.913116141937977	28.443283687619314	21.688709296948865
31	28.429017557976486	25.377192087804275	22.659869815309936	23.533920538909303
32	30.696581322257476	25.251362321524773	24.51046214125846	19.541594214959286
33	28.520057990565583	25.553891868994384	22.883685366767804	23.042364773672233
34	23.222505945784953	26.475684070301988	26.47155440119485	23.83025558271821
35	25.37249928200071	23.48417679739155	27.43138961488333	23.711934305724412
36	30.909822417971316	24.445513708937167	24.198359269949588	20.446304603141925
37	25.30949054274489	27.691558768632785	26.30943360336781	20.689517085254515
38	25.67252599970842	29.000038168153868	23.185338923820744	22.142096908316965
39	24.383944096794437	27.870260813632413	25.066027777656114	22.679767311917036
40	27.831341810834875	24.156061446973485	25.335269689292456	22.677327052899184
41	21.360025178467403	22.793583495339416	27.281032116937208	28.565359209255963
42	24.5094610093537	25.003582175096717	24.867615948281525	25.619340867268054
43	24.03142052483089	24.29046340518749	26.61634310292074	25.061772967060886
44	23.213495758642114	26.951597149527185	26.925380007771288	22.909527084059413
45	22.356339135935567	28.189809603482942	24.19272790298531	25.261123357596183
46	23.858725271259818	28.32108302449457	25.24279012959027	22.577401574655344
47	24.637105327210577	24.71613217694256	26.78397012622396	23.862792369622905
48	25.067466904769205	22.182267325995454	28.563294374702398	24.186971394532943
49	23.53598537346287	22.307533955578528	29.38153199461141	24.774948676347194
50	22.42172556346519	27.568732398067564	26.772957675271602	23.23658436319564
51	21.254968899211672	28.46931311714307	24.524039992716766	25.751677990928496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1044.0
1	1682.0
2	2320.0
3	6998.0
4	11676.0
5	8542.5
6	5409.0
7	5788.5
8	6168.0
9	6648.0
10	7128.0
11	7626.5
12	8125.0
13	8028.0
14	7931.0
15	8048.5
16	8166.0
17	7869.5
18	7573.0
19	7625.0
20	7677.0
21	7444.5
22	7212.0
23	6947.5
24	6683.0
25	6599.0
26	7969.0
27	9423.0
28	10623.0
29	11823.0
30	13396.5
31	14970.0
32	16851.5
33	18733.0
34	22058.0
35	25383.0
36	28107.0
37	30831.0
38	36301.0
39	41771.0
40	57522.5
41	73274.0
42	90718.0
43	108162.0
44	111806.5
45	115451.0
46	115383.0
47	115315.0
48	122085.0
49	128855.0
50	134720.5
51	140586.0
52	131274.0
53	121962.0
54	109819.0
55	97676.0
56	88745.0
57	79814.0
58	79557.0
59	79300.0
60	74816.5
61	70333.0
62	64772.5
63	59212.0
64	51744.5
65	44277.0
66	39444.5
67	34612.0
68	31055.0
69	27498.0
70	22846.0
71	18194.0
72	16491.0
73	14788.0
74	12311.0
75	7855.0
76	5876.0
77	4506.0
78	3136.0
79	2535.0
80	1934.0
81	1423.0
82	912.0
83	562.5
84	213.0
85	223.5
86	234.0
87	162.5
88	91.0
89	65.0
90	39.0
91	36.0
92	33.0
93	21.5
94	10.0
95	8.5
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1598191.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.182426721722955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7331397067551	17.101238158529615
2	7.434229090694314	3.1495002589226675
3	2.414880545724294	1.5345909060455758
4	1.140855823985302	0.9666437956647806
5	0.6890931029519817	0.7298332078862521
6	0.5071007771354621	0.6444975031320413
7	0.3849300551419668	0.5707626880223441
8	0.29345225037072553	0.4972824631842073
9	0.23735156388042838	0.4524913898265167
>10	3.372012941246188	17.999133820318246
>50	1.8305437184428803	28.675554350965804
>100	0.9561648677699753	25.89656992667959
>500	0.0032714816626517613	0.42756817004388675
>1k	0.002676666814896896	0.9304820792026236
>5k	2.9740742387743285E-4	0.4238512815758464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6728	0.42097596595150394	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3336	0.20873600214242224	No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT	2016	0.12614261999973722	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT	1620	0.10136460535693168	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2514148809497739E-4	0.0	0.0	0.09842378038669972	0.0
2	1.2514148809497739E-4	0.0	0.0	0.3233656052374216	0.0
3	1.2514148809497739E-4	0.0	0.0	0.4670906043145031	0.0
4	1.2514148809497739E-4	0.0	0.0	0.9003304360993147	0.0
5	1.2514148809497739E-4	0.0	0.0	1.4166642159791916	0.0
6	1.2514148809497739E-4	0.0	0.0	2.326442834429677	0.0
7	1.2514148809497739E-4	0.0	0.0	2.703118713595559	0.0
8	1.2514148809497739E-4	0.0	0.0	3.816127108712288	0.0
9	1.2514148809497739E-4	0.0	0.0	4.275458940764902	0.0
10	1.2514148809497739E-4	0.0	0.0	5.040824281953784	0.0
11	1.2514148809497739E-4	0.0	0.0	5.749312816803498	0.0
12	1.2514148809497739E-4	0.0	0.0	6.307256141474955	0.0
13	1.2514148809497739E-4	0.0	0.0	6.566424163319653	0.0
14	1.2514148809497739E-4	0.0	0.0	6.66616192933135	0.0
15	1.2514148809497739E-4	0.0	0.0	6.7967470721584595	0.0
16	1.2514148809497739E-4	0.0	0.0	7.066426979003135	0.0
17	1.2514148809497739E-4	0.0	0.0	7.336857734776381	0.0
18	1.2514148809497739E-4	0.0	0.0	7.733431110549365	0.0
19	1.2514148809497739E-4	0.0	0.0	7.906376647096623	0.0
20	1.2514148809497739E-4	0.0	0.0	8.0963414260248	0.0
21	1.2514148809497739E-4	0.0	0.0	8.332796267780259	0.0
22	1.2514148809497739E-4	0.0	0.0	8.566935992005961	0.0
23	1.2514148809497739E-4	0.0	0.0	8.826229155338755	0.0
24	1.2514148809497739E-4	0.0	0.0	9.02764438042762	0.0
25	1.2514148809497739E-4	0.0	0.0	9.196898243076078	0.0
26	1.2514148809497739E-4	0.0	0.0	9.359519606855502	0.0
27	1.2514148809497739E-4	0.0	0.0	9.50831283620043	0.0
28	1.2514148809497739E-4	0.0	0.0	9.659984319771542	0.0
29	1.2514148809497739E-4	0.0	0.0	9.818038019235498	0.0
30	1.2514148809497739E-4	0.0	0.0	10.031028831973149	0.0
31	1.2514148809497739E-4	0.0	0.0	10.222557879502512	0.0
32	1.2514148809497739E-4	0.0	0.0	10.408580701555696	0.0
33	1.2514148809497739E-4	0.0	0.0	10.583465931168428	0.0
34	1.2514148809497739E-4	0.0	0.0	10.760916561287106	0.0
35	1.2514148809497739E-4	0.0	0.0	10.966148601762868	0.0
36	1.2514148809497739E-4	0.0	0.0	11.149105457357726	0.0
37	1.2514148809497739E-4	0.0	0.0	11.338507099589473	0.0
38	1.2514148809497739E-4	0.0	0.0	11.512578909529587	0.0
39	1.2514148809497739E-4	0.0	0.0	11.693721213547066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTC	20	7.0341147E-4	45.0	14
CCGTAAC	20	7.0341147E-4	45.0	37
CGTACTT	20	7.0341147E-4	45.0	29
ATACGTG	20	7.0341147E-4	45.0	1
TGTACCC	25	3.8914914E-5	45.0	31
AATACGG	65	0.0	44.999996	2
TAGTACG	65	0.0	44.999996	1
TAATGCG	105	0.0	42.857143	1
AGGGTAC	745	0.0	42.583893	6
TGCGTTT	90	0.0	42.5	28
ACGTTAG	85	0.0	42.35294	1
AACGGGA	455	0.0	41.538464	4
TAACGAG	65	0.0	41.53846	1
CGTTAGG	185	0.0	41.351353	2
CTCATGC	305	0.0	41.311478	23
CGTTTTT	5000	0.0	41.085003	1
ACACGAC	615	0.0	40.97561	26
CGTAAGG	165	0.0	40.90909	2
CACGACC	615	0.0	40.609756	27
CGCATCG	50	1.0822987E-9	40.5	21
>>END_MODULE