FastQCFastQC Report
Thu 26 May 2016
SRR1546990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546990_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2498810
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301721.2074547484602671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT216750.043.629761
GCGATAC1600.043.5937549
TCTAGCG1500.039.0000041
CGATACA750.039.00000410
TATAGCG1000.038.251
GGGCGAT56200.037.5934147
AGGGCGA28200.037.579796
GTTTTTT258650.037.1708872
TAGGGCG11200.037.165185
TACGGGA7050.036.702134
CACGACC6450.036.62790727
AGGGTAC11650.036.3090136
ACGGGAT7950.036.2264145
CGTAAGG3700.035.878382
GGCGATT12050.035.8506248
GGCGATA10550.035.8293848
GACGGGA8650.035.635844
AAGGGAT27450.035.5737725
TAACGGG6200.035.5645143
TAGGGAT22150.035.3498885
Produced by FastQC (version 0.11.5)