##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546990_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2498810
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6164170144989	31.0	31.0	33.0	30.0	34.0
2	31.991672436079575	31.0	31.0	34.0	30.0	34.0
3	31.952233663223694	33.0	31.0	34.0	30.0	34.0
4	35.72169872859481	37.0	35.0	37.0	33.0	37.0
5	35.70027212953366	37.0	35.0	37.0	33.0	37.0
6	35.768715108391596	37.0	35.0	37.0	33.0	37.0
7	36.13821819185932	37.0	35.0	37.0	35.0	37.0
8	36.085108911842035	37.0	35.0	37.0	35.0	37.0
9	37.86061725381281	39.0	38.0	39.0	35.0	39.0
10	37.32758433014115	39.0	37.0	39.0	34.0	39.0
11	37.126157250851406	39.0	37.0	39.0	33.0	39.0
12	36.823376727322206	39.0	35.0	39.0	33.0	39.0
13	36.75105910413357	39.0	35.0	39.0	33.0	39.0
14	37.875102148622744	40.0	36.0	41.0	33.0	41.0
15	37.94609634185873	40.0	36.0	41.0	33.0	41.0
16	38.01024167503732	40.0	36.0	41.0	33.0	41.0
17	37.971075832096076	40.0	36.0	41.0	33.0	41.0
18	37.79633065339102	39.0	36.0	41.0	33.0	41.0
19	37.64474689952417	39.0	36.0	41.0	33.0	41.0
20	37.40914595347385	39.0	35.0	41.0	33.0	41.0
21	37.34600589880783	39.0	35.0	41.0	33.0	41.0
22	37.27413088630188	39.0	35.0	41.0	33.0	41.0
23	37.17961189526214	39.0	35.0	41.0	32.0	41.0
24	37.06207034548445	39.0	35.0	41.0	32.0	41.0
25	36.96570927761614	39.0	35.0	41.0	32.0	41.0
26	36.83050452015159	38.0	35.0	41.0	32.0	41.0
27	36.734451999151595	38.0	35.0	41.0	31.0	41.0
28	36.73003189518211	38.0	35.0	41.0	31.0	41.0
29	36.697636074771594	38.0	35.0	41.0	31.0	41.0
30	36.57492086233047	38.0	35.0	41.0	31.0	41.0
31	36.382173114402455	38.0	35.0	41.0	31.0	41.0
32	36.13625485731208	38.0	35.0	41.0	30.0	41.0
33	35.78939975428304	38.0	35.0	41.0	28.0	41.0
34	35.4169320596604	38.0	35.0	41.0	25.0	41.0
35	35.18605416178101	38.0	35.0	41.0	24.0	41.0
36	35.02378332086073	38.0	35.0	41.0	23.0	41.0
37	34.932116887638514	38.0	34.0	41.0	23.0	41.0
38	34.82729979470228	38.0	34.0	41.0	23.0	41.0
39	34.73228096573969	38.0	34.0	41.0	22.0	41.0
40	34.614578539384745	38.0	34.0	41.0	21.0	41.0
41	34.54381645663336	38.0	34.0	40.0	21.0	41.0
42	34.456957511775606	38.0	34.0	40.0	21.0	41.0
43	34.349860533614	38.0	34.0	40.0	20.0	41.0
44	34.14001144544803	38.0	34.0	40.0	18.0	41.0
45	34.068175251419675	37.0	33.0	40.0	20.0	41.0
46	34.01955770946971	37.0	33.0	40.0	20.0	41.0
47	33.98430052705088	37.0	33.0	40.0	20.0	41.0
48	33.87527543110521	37.0	33.0	40.0	19.0	41.0
49	33.790860849764485	37.0	33.0	40.0	18.0	41.0
50	33.68129389589445	36.0	33.0	40.0	18.0	41.0
51	33.30676041795895	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	5.0
13	6.0
14	17.0
15	64.0
16	156.0
17	437.0
18	1104.0
19	2210.0
20	3925.0
21	6385.0
22	9871.0
23	15132.0
24	24266.0
25	40281.0
26	56087.0
27	58833.0
28	53568.0
29	52109.0
30	55707.0
31	63887.0
32	77941.0
33	99012.0
34	159920.0
35	237508.0
36	185335.0
37	236630.0
38	352290.0
39	705621.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.799536579411804	24.135208359178968	26.310803942676714	17.75445111873252
2	37.16605104029518	24.83754267031107	25.206718397957427	12.789687891436325
3	28.11674356993929	24.16170096966156	34.50390385823653	13.217651602162631
4	24.10387344375923	27.40424442034408	33.98313597272302	14.508746163173672
5	21.688363661102684	31.10312508754167	33.406501494711485	13.802009756644162
6	20.41035532913667	39.40127500690328	30.301663591869733	9.886706072090314
7	83.32034048206947	2.9865816128477154	12.14041883936754	1.5526590657152803
8	83.21785169740797	2.5256822247389756	11.798376026988846	2.4580900508642114
9	77.66760978225635	4.638567958348173	14.381685682384814	3.312136577010657
10	37.35257982799813	26.430861089878782	22.38149359094929	13.835065491173799
11	29.99047546632197	25.863591069349013	28.193980334639292	15.951953129689734
12	25.924460042980456	22.18439977429256	33.33742861602123	18.55371156670575
13	22.345276351543333	26.085376639280298	34.91714055890604	16.65220645027033
14	20.31030770646828	27.752410147230083	34.49662039130626	17.44066175499538
15	19.074599509366458	24.37268139634466	39.63578663443799	16.91693245985089
16	21.329672924311975	22.5929542462212	37.875348665965	18.202024163501825
17	21.43732416630316	23.17283026720719	33.30421280529532	22.08563276119433
18	22.66574889647472	23.39109416082055	34.62352079589885	19.31963614680588
19	24.541001516721963	25.926300919237555	30.647148042468213	18.88554952157227
20	25.501298618142236	25.09498521296137	30.565829334763343	18.837886834133048
21	23.89409358854815	26.146685822451488	31.817024903854236	18.142195685146127
22	23.20600605888403	23.761070269448258	31.45109071918233	21.58183295248538
23	21.35984728730876	26.607785305805564	30.814147534226294	21.218219872659386
24	20.727306197750128	25.330417278624623	34.91341878734278	19.028857736282472
25	21.64390249758885	25.430905110832757	32.83242823584026	20.092764155738134
26	20.307586411131698	27.861301979742358	31.599641429320357	20.231470179805587
27	21.118932611923274	26.153529079842002	31.956651366050238	20.77088694218448
28	20.592682116687545	26.281790132102884	33.623284683509354	19.502243067700224
29	21.048298990319392	24.999059552346917	32.620447332930475	21.332194124403216
30	21.740188329644912	26.346500934444794	32.163549849728476	19.74976088618182
31	23.519395232130496	26.259779655115835	29.76969037261736	20.451134740136304
32	24.762987181898584	26.283751065507182	29.607853338188978	19.345408414405256
33	24.380285015667457	26.97628070961778	28.480476706912487	20.162957567802273
34	22.2077708989479	28.098815035957113	29.315394127604737	20.378019937490247
35	22.953325783072742	27.892957047554635	28.67332850436808	20.480388665004543
36	25.047242487424015	27.91032531484987	26.60074195316971	20.44169024455641
37	23.59859293023479	28.542746347261293	27.94542202088194	19.91323870162197
38	23.043768833964968	28.465389525414096	27.45390806023667	21.03693358038426
39	23.090270968981237	27.14732212533166	27.65024151496112	22.112165390725984
40	23.75606788831484	26.207874948475474	28.142515837538667	21.89354132567102
41	20.86201031691085	25.868353336188026	29.182010637063243	24.08762570983788
42	22.546051920714262	26.971478423729696	27.89279697135837	22.58967268419768
43	22.189322117327848	26.26602262677034	29.471228304673026	22.073426951228786
44	22.476258699140793	27.713391574389405	28.26557441342079	21.544775313049012
45	21.512640016647925	27.76061405228889	27.698384430989154	23.028361500074034
46	22.63625485731208	27.908364381445566	27.245568890792015	22.209811870450334
47	22.316342579067637	26.909568954822493	28.84789159639989	21.926196869709983
48	22.429676526026388	26.36775104949956	28.932651942324544	22.2699204821495
49	22.330429284339345	25.93914703398818	28.885429464425066	22.84499421724741
50	21.389621459814872	27.268459786858546	29.13346753054454	22.208451222782045
51	21.114370440329598	27.727798432053657	27.983240022250595	23.174591105366154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1161.0
1	1911.5
2	2662.0
3	24918.0
4	47174.0
5	33114.0
6	19054.0
7	19357.0
8	19660.0
9	20815.0
10	21970.0
11	22895.0
12	23820.0
13	23716.5
14	23613.0
15	23406.0
16	23199.0
17	22725.5
18	22252.0
19	21057.5
20	19863.0
21	19605.0
22	19347.0
23	19360.5
24	19374.0
25	19927.5
26	21828.5
27	23176.0
28	26288.0
29	29400.0
30	31732.5
31	34065.0
32	40802.0
33	47539.0
34	53404.5
35	59270.0
36	66368.5
37	73467.0
38	81741.0
39	90015.0
40	102651.5
41	115288.0
42	131546.0
43	147804.0
44	157702.5
45	167601.0
46	184262.0
47	200923.0
48	206278.0
49	211633.0
50	206017.5
51	200402.0
52	181584.0
53	162766.0
54	146231.5
55	129697.0
56	121845.5
57	113994.0
58	105860.5
59	97727.0
60	87876.5
61	78026.0
62	70521.0
63	63016.0
64	56533.5
65	50051.0
66	43940.0
67	37829.0
68	31746.0
69	25663.0
70	22430.5
71	19198.0
72	16391.0
73	13584.0
74	11757.0
75	8005.5
76	6081.0
77	4714.0
78	3347.0
79	2634.0
80	1921.0
81	1415.5
82	910.0
83	733.5
84	557.0
85	373.5
86	190.0
87	128.5
88	67.0
89	44.0
90	21.0
91	16.5
92	12.0
93	9.0
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2498810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.530836777116026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7877561738888	22.676066940520933
2	7.499533046141845	4.429349725804052
3	2.9657189275436853	2.627404847282885
4	1.785776502737166	2.109418976909613
5	1.23105817173828	1.817708896636901
6	0.9711898104200837	1.7208028662668247
7	0.7556710122630863	1.5620918124237473
8	0.6670705649339178	1.5759321577505683
9	0.524889685712708	1.3950388471296338
>10	5.8805526961688175	38.83837514832849
>50	0.8691880523842781	16.635105096605827
>100	0.058289260996962064	2.5149644122505395
>500	0.0019285554577272027	0.4227454943579888
>1k	0.001239785651396059	0.44760091170332483
>5k	0.0	0.0
>10k+	1.3775396126622876E-4	1.2273938660286028
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30172	1.2074547484602671	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.053425430504920345	0.0
2	0.0	0.0	0.0	0.17276223482377612	0.0
3	0.0	0.0	0.0	0.31198850652910787	0.0
4	0.0	0.0	0.0	0.5276911810021571	0.0
5	0.0	0.0	0.0	0.9328440337600697	0.0
6	0.0	0.0	0.0	1.8443579143672388	0.0
7	0.0	0.0	0.0	2.3234259507525583	0.0
8	0.0	0.0	0.0	3.3609598168728314	0.0
9	0.0	0.0	0.0	3.995061649345088	0.0
10	0.0	0.0	0.0	4.831259679607493	0.0
11	0.0	0.0	0.0	5.433106158531461	0.0
12	0.0	0.0	0.0	5.932103681352324	0.0
13	0.0	0.0	0.0	6.238729635306406	0.0
14	0.0	0.0	0.0	6.415333698840648	0.0
15	0.0	0.0	0.0	6.566285551922715	0.0
16	0.0	0.0	0.0	6.792233102957008	0.0
17	0.0	0.0	0.0	7.040991511959692	0.0
18	0.0	0.0	0.0	7.375110552623049	0.0
19	0.0	0.0	0.0	7.565521188085529	0.0
20	0.0	0.0	0.0	7.7761014242779565	0.0
21	0.0	0.0	0.0	8.019937490245356	0.0
22	0.0	0.0	0.0	8.287664928505968	0.0
23	0.0	0.0	0.0	8.589688691817305	0.0
24	0.0	0.0	0.0	8.83800689128025	0.0
25	0.0	0.0	0.0	9.048467070325476	0.0
26	0.0	0.0	0.0	9.255245496856503	0.0
27	0.0	0.0	0.0	9.470267847495407	0.0
28	0.0	0.0	0.0	9.68957223638452	0.0
29	0.0	0.0	0.0	9.926004778274459	0.0
30	0.0	0.0	0.0	10.263485419059473	0.0
31	0.0	0.0	0.0	10.529131866768582	0.0
32	0.0	0.0	0.0	10.799220428924167	0.0
33	0.0	0.0	0.0	11.058944057371308	0.0
34	0.0	0.0	0.0	11.32699164802446	0.0
35	0.0	0.0	0.0	11.6098863058816	0.0
36	0.0	0.0	0.0	11.886938182574905	0.0
37	0.0	0.0	0.0	12.174395012025725	0.0
38	0.0	0.0	0.0	12.487544070977785	0.0
39	0.0	0.0	0.0	12.896578771495232	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21675	0.0	43.62976	1
GCGATAC	160	0.0	43.593754	9
TCTAGCG	150	0.0	39.000004	1
CGATACA	75	0.0	39.000004	10
TATAGCG	100	0.0	38.25	1
GGGCGAT	5620	0.0	37.593414	7
AGGGCGA	2820	0.0	37.57979	6
GTTTTTT	25865	0.0	37.170887	2
TAGGGCG	1120	0.0	37.16518	5
TACGGGA	705	0.0	36.70213	4
CACGACC	645	0.0	36.627907	27
AGGGTAC	1165	0.0	36.309013	6
ACGGGAT	795	0.0	36.226414	5
CGTAAGG	370	0.0	35.87838	2
GGCGATT	1205	0.0	35.850624	8
GGCGATA	1055	0.0	35.829384	8
GACGGGA	865	0.0	35.63584	4
AAGGGAT	2745	0.0	35.573772	5
TAACGGG	620	0.0	35.564514	3
TAGGGAT	2215	0.0	35.349888	5
>>END_MODULE