FastQCFastQC Report
Thu 26 May 2016
SRR1546989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546989_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1797117
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90360.5028053265313277No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT253.8916856E-545.0000046
CTATACG207.034349E-445.01
AATACCG207.034349E-445.01
CGAATAT1050.040.71428714
CATATGC9100.039.5604433
CGATACA403.4596815E-739.37510
CGTAAGG2300.039.1304322
CGTTTTT56550.039.031831
AGGGCGA23900.038.31596
CGTTAGG2250.038.02
GGGCGAT44250.037.983057
CACGACC7400.037.70270527
ATATGCT9500.037.65789434
TATTTCG301.140178E-437.4999961
AACGTCG301.140178E-437.49999632
AACCGTA301.140178E-437.49999643
TTATCCG301.140178E-437.4999961
CGGTAGT5550.037.297312
GCGATAT2300.037.1739129
TAGACGG2800.036.9642832