##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546989_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1797117
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63705034229825	31.0	31.0	33.0	30.0	34.0
2	32.02784904933847	33.0	31.0	34.0	30.0	34.0
3	32.135436368361106	33.0	31.0	34.0	30.0	34.0
4	35.8140404881819	37.0	35.0	37.0	35.0	37.0
5	35.77396574624802	37.0	35.0	37.0	35.0	37.0
6	35.82282567022626	37.0	35.0	37.0	35.0	37.0
7	36.11556175808253	37.0	35.0	37.0	35.0	37.0
8	36.03850277972998	37.0	35.0	37.0	35.0	37.0
9	37.8079012106613	39.0	38.0	39.0	35.0	39.0
10	37.3728722170009	39.0	37.0	39.0	34.0	39.0
11	37.09466217280233	39.0	37.0	39.0	33.0	39.0
12	36.62226610732635	39.0	35.0	39.0	33.0	39.0
13	36.49003932409521	39.0	35.0	39.0	32.0	39.0
14	37.55444971028597	40.0	35.0	41.0	33.0	41.0
15	37.6569310734916	40.0	35.0	41.0	33.0	41.0
16	37.73919728097837	40.0	35.0	41.0	33.0	41.0
17	37.682633351083986	40.0	35.0	41.0	33.0	41.0
18	37.57610550676444	39.0	36.0	41.0	33.0	41.0
19	37.521845266613134	39.0	35.0	41.0	33.0	41.0
20	37.39665530958752	39.0	35.0	41.0	32.0	41.0
21	37.328729292527974	39.0	35.0	41.0	32.0	41.0
22	37.24255348983956	39.0	35.0	41.0	32.0	41.0
23	37.14465224022699	39.0	35.0	41.0	32.0	41.0
24	37.04529421289766	39.0	35.0	41.0	32.0	41.0
25	36.90634499590177	39.0	35.0	41.0	31.0	41.0
26	36.75702361059408	38.0	35.0	41.0	31.0	41.0
27	36.65630785307801	38.0	35.0	41.0	31.0	41.0
28	36.67932972644519	38.0	35.0	41.0	31.0	41.0
29	36.67335905230433	38.0	35.0	41.0	31.0	41.0
30	36.590312149960184	38.0	35.0	41.0	31.0	41.0
31	36.473892350915385	38.0	35.0	41.0	31.0	41.0
32	36.384180885273466	38.0	35.0	41.0	30.0	41.0
33	36.24969158936229	38.0	35.0	41.0	30.0	41.0
34	36.07191907928087	38.0	35.0	41.0	30.0	41.0
35	35.93587284522933	38.0	35.0	41.0	29.0	41.0
36	35.84975992102907	38.0	35.0	41.0	29.0	41.0
37	35.75253531072267	38.0	35.0	41.0	29.0	41.0
38	35.64747704239624	38.0	35.0	41.0	29.0	41.0
39	35.53616375561524	38.0	35.0	40.0	28.0	41.0
40	35.38997460933261	38.0	35.0	40.0	27.0	41.0
41	35.308123511157035	37.0	34.0	40.0	27.0	41.0
42	35.219643462278746	37.0	34.0	40.0	27.0	41.0
43	35.08098359761774	37.0	34.0	40.0	27.0	41.0
44	34.93015758016868	37.0	34.0	40.0	26.0	41.0
45	34.82780475617336	36.0	34.0	40.0	26.0	41.0
46	34.77054192910089	36.0	34.0	40.0	26.0	41.0
47	34.68879822515729	36.0	34.0	40.0	26.0	41.0
48	34.576089369807306	36.0	34.0	40.0	26.0	41.0
49	34.481606929320684	36.0	34.0	40.0	26.0	41.0
50	34.368109032411354	35.0	34.0	40.0	25.0	41.0
51	33.99415897796304	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	7.0
14	31.0
15	61.0
16	148.0
17	333.0
18	751.0
19	1442.0
20	2447.0
21	3956.0
22	5992.0
23	8788.0
24	12765.0
25	18283.0
26	23359.0
27	27472.0
28	30440.0
29	34601.0
30	39985.0
31	47683.0
32	59084.0
33	77153.0
34	136106.0
35	203755.0
36	139286.0
37	184665.0
38	265613.0
39	472597.0
40	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.305102561491545	24.069217530077342	27.36911397532826	13.256565933102854
2	33.7905100224415	25.732603942870718	26.779669882372716	13.69721615231507
3	30.62388258527408	25.545137016677266	29.927044260334746	13.903936137713904
4	26.202578908329283	28.81604258376054	29.228870463080593	15.75250804482958
5	23.442046344228004	32.764700350617126	28.294262421422754	15.49899088373211
6	22.76312560617923	41.121696584028754	25.70172114559041	10.413456664201608
7	87.52268216259709	3.777272153120804	6.922142520492544	1.7779031637895584
8	87.3559707019632	3.327718785143093	6.62294107729213	2.693369435601577
9	81.82127262721347	4.955158734795787	9.52965221518688	3.693916422803858
10	47.45522968176251	23.51382798114981	15.330164925266413	13.700777411821266
11	37.20854012287458	25.492274570882138	21.51490414925684	15.78428115698644
12	31.812786813546364	21.95666726206474	26.85139587461473	19.379150049774164
13	25.52783152126434	26.75574267006544	29.00061598660521	18.715809822065008
14	22.483566734942688	27.70337156679281	30.31939489749415	19.493666800770345
15	21.61461941543038	22.647050804149092	36.71892258545215	19.019407194968384
16	24.929818147621997	20.8525655257838	33.709101855917005	20.508514470677202
17	25.08540067229902	21.41641306603855	27.998900461127462	25.499285800534967
18	25.712460568788785	22.241790601279714	30.25963251140577	21.78611631852573
19	29.071841176729173	23.860049178767994	25.949952062108366	21.118157582394467
20	29.7086388921812	24.681364652384904	24.88196372300746	20.728032732426435
21	28.260986902911718	24.97444518080904	26.657863678324784	20.106704237954457
22	26.833478287724173	23.126485365170993	26.406015857620847	23.63402048948399
23	25.498951932456258	25.32879050167574	25.921128117980075	23.251129447887923
24	25.362511177625052	23.614099694121197	29.80701868603992	21.216370442213837
25	25.417655055291338	23.360916401102433	28.346401486380685	22.875027057225545
26	22.45273958234216	26.350482467196066	26.964187640537595	24.232590309924174
27	23.39163226434339	25.192127168125396	27.64249628710874	23.773744280422477
28	22.669197386703257	25.96686804476281	29.515273629930604	21.848660938603327
29	23.337935148351498	24.377934213520877	28.841694781141126	23.442435856986496
30	24.94539865796161	24.58281792448683	28.6491641890873	21.82261922846426
31	27.24229975009974	24.69221536494285	25.376422347571136	22.68906253738627
32	29.081857219090356	24.15235068167515	25.314267240252025	21.45152485898247
33	27.146368322151538	24.776183186737423	24.607802385710002	23.46964610540104
34	24.370366537070208	25.574072250165123	27.325989348495394	22.72957186426927
35	23.644092176524957	26.114549024910456	27.4841315284425	22.75722727012209
36	27.189659883023754	26.376245953936223	24.66439302505068	21.769701137989347
37	24.823815032632822	27.766695212387393	25.279767538785734	22.129722216194047
38	25.407805946969507	27.529927099905017	24.503802479193062	22.558464473932418
39	23.749650134075857	26.971087580830854	24.80712162869752	24.472140656395773
40	25.96397452141402	24.20126235520559	26.239248752307166	23.595514371073225
41	22.328206788984804	23.84736219177716	26.91522032232737	26.909210696910662
42	24.439087716603872	24.749473740440937	25.295848851243406	25.51558969171178
43	23.591452309448968	23.569917818372428	27.36777850301344	25.47085136916517
44	23.331647299535867	24.997815946318465	26.954616755614687	24.715919998530982
45	22.314239974358934	26.16334940908132	25.951899625900822	25.570510990658928
46	24.43124181675428	25.341533133346356	26.4367317208618	23.790493329037567
47	23.438596374081374	25.102149720914106	27.740152700130267	23.719101204874253
48	24.44765699729066	23.86806201265694	28.14953060930368	23.53475038074872
49	24.19324952131664	23.400813636507806	27.957500819367908	24.44843602280764
50	23.184689700225416	25.53517661899587	27.413574074475953	23.86655960630276
51	22.47694501804835	26.172642070605306	26.29923371711469	25.051179194231647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	963.0
1	1795.0
2	2627.0
3	9581.0
4	16535.0
5	11585.5
6	6636.0
7	6699.5
8	6763.0
9	7099.0
10	7435.0
11	7682.0
12	7929.0
13	7958.5
14	7988.0
15	7972.5
16	7957.0
17	7782.0
18	7607.0
19	7529.0
20	7451.0
21	7381.5
22	7312.0
23	7502.0
24	7692.0
25	8417.5
26	10796.5
27	12450.0
28	14985.0
29	17520.0
30	19286.0
31	21052.0
32	24621.0
33	28190.0
34	31046.0
35	33902.0
36	36706.5
37	39511.0
38	46304.0
39	53097.0
40	63215.5
41	73334.0
42	83714.5
43	94095.0
44	97856.0
45	101617.0
46	111271.0
47	120925.0
48	126616.0
49	132307.0
50	131669.0
51	131031.0
52	124200.0
53	117369.0
54	110691.0
55	104013.0
56	102995.5
57	101978.0
58	100820.0
59	99662.0
60	95991.0
61	92320.0
62	86621.5
63	80923.0
64	74345.5
65	67768.0
66	59523.0
67	51278.0
68	44810.0
69	38342.0
70	33617.5
71	28893.0
72	24014.5
73	19136.0
74	16825.0
75	11546.5
76	8579.0
77	6615.5
78	4652.0
79	3420.5
80	2189.0
81	1855.0
82	1521.0
83	1069.0
84	617.0
85	412.5
86	208.0
87	131.5
88	55.0
89	32.0
90	9.0
91	10.0
92	11.0
93	8.5
94	6.0
95	4.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1797117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.7826000701965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3729766712437	17.627575837415893
2	6.5026185859143455	2.962931173038265
3	2.3630164316769093	1.6150697496659352
4	1.2992096651045872	1.18397496829647
5	0.9625997306419171	1.0965262345446836
6	0.7397765066892387	1.0112419375936783
7	0.6008753344887897	0.9582651706392553
8	0.5147849851034116	0.9382512350202474
9	0.4273097842741528	0.8761705129079967
>10	7.384871790018872	42.73857797881386
>50	1.75800254770367	25.605723313185607
>100	0.07050659554523173	2.2959755273574007
>500	0.0024652654251401335	0.39029167076631394
>1k	7.395796275420401E-4	0.19191633889890555
>5k	2.465265425140133E-4	0.5075083518555782
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9036	0.5028053265313277	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05386405003124449	0.0
2	0.0	0.0	0.0	0.1471801780295885	0.0
3	0.0	0.0	0.0	0.22691900416055272	0.0
4	0.0	0.0	0.0	0.4389808788186857	0.0
5	0.0	0.0	0.0	0.7494781920153223	0.0
6	0.0	0.0	0.0	1.3935097158393137	0.0
7	0.0	0.0	0.0	1.6816935124424286	0.0
8	0.0	0.0	0.0	2.4646698016879256	0.0
9	0.0	0.0	0.0	2.8771081682494795	0.0
10	0.0	0.0	0.0	3.4781819992799576	0.0
11	0.0	0.0	0.0	3.9829348896037375	0.0
12	0.0	0.0	0.0	4.388083803113543	0.0
13	0.0	0.0	0.0	4.600312611811028	0.0
14	0.0	0.0	0.0	4.703088335372711	0.0
15	0.0	0.0	0.0	4.81170675031175	0.0
16	0.0	0.0	0.0	4.998617229707359	0.0
17	0.0	0.0	0.0	5.183301921911595	0.0
18	0.0	0.0	0.0	5.448226242364854	0.0
19	0.0	0.0	0.0	5.587393586505497	0.0
20	0.0	0.0	0.0	5.74358820266015	0.0
21	0.0	0.0	0.0	5.92832853954417	0.0
22	0.0	0.0	0.0	6.117075293372663	0.0
23	0.0	0.0	0.0	6.326188000002226	0.0
24	0.0	0.0	0.0	6.505030000829105	0.0
25	0.0	0.0	0.0	6.660389946786993	0.0
26	0.0	0.0	0.0	6.7996685802872046	0.0
27	0.0	0.0	0.0	6.949074545508167	0.0
28	0.0	0.0	0.0	7.092693464031557	0.0
29	0.0	0.0	0.0	7.252059826933917	0.0
30	0.0	0.0	0.0	7.481983643802824	0.0
31	0.0	0.0	0.0	7.654927308572564	0.0
32	0.0	0.0	0.0	7.836941056147151	0.0
33	0.0	0.0	0.0	8.014892742097482	0.0
34	5.564467978434348E-5	0.0	0.0	8.197908093908188	0.0
35	5.564467978434348E-5	0.0	0.0	8.410415126004596	0.0
36	5.564467978434348E-5	0.0	0.0	8.593486122495085	0.0
37	5.564467978434348E-5	0.0	0.0	8.77995144445242	0.0
38	1.1128935956868696E-4	0.0	0.0	8.990900425514866	0.0
39	1.1128935956868696E-4	0.0	0.0	9.316811315011766	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAAT	25	3.8916856E-5	45.000004	6
CTATACG	20	7.034349E-4	45.0	1
AATACCG	20	7.034349E-4	45.0	1
CGAATAT	105	0.0	40.714287	14
CATATGC	910	0.0	39.56044	33
CGATACA	40	3.4596815E-7	39.375	10
CGTAAGG	230	0.0	39.130432	2
CGTTTTT	5655	0.0	39.03183	1
AGGGCGA	2390	0.0	38.3159	6
CGTTAGG	225	0.0	38.0	2
GGGCGAT	4425	0.0	37.98305	7
CACGACC	740	0.0	37.702705	27
ATATGCT	950	0.0	37.657894	34
TATTTCG	30	1.140178E-4	37.499996	1
AACGTCG	30	1.140178E-4	37.499996	32
AACCGTA	30	1.140178E-4	37.499996	43
TTATCCG	30	1.140178E-4	37.499996	1
CGGTAGT	555	0.0	37.2973	12
GCGATAT	230	0.0	37.173912	9
TAGACGG	280	0.0	36.964283	2
>>END_MODULE