FastQCFastQC Report
Thu 26 May 2016
SRR1546988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546988_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1267407
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92920.7331504402295395No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATA207.033561E-445.027
CGTTTTT69600.042.219831
TCGATAG501.0822987E-940.51
GGGCGAT32650.039.8315477
GACGGGA4950.039.5454524
GGCGATT8400.038.8392878
AGGGCGA15550.038.7781376
TACGGGA2100.038.571434
TCGTTAG356.2487998E-638.5714261
TTACGGG2400.038.4374963
GACACGA13150.038.1558925
ACGGGAT2300.038.1521725
TCAAGCG13400.038.1156717
CACGACC13250.038.03773527
ACACGAC13250.037.86792426
GCGAGAC13450.037.8066921
TAGGGCG5600.037.7678575
ACGACCA13500.037.528
CGTTCGA301.13998816E-437.49999614
TAATGCG301.13998816E-437.4999961
Produced by FastQC (version 0.11.5)