##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546988_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1267407
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.582923244072347	31.0	31.0	33.0	30.0	34.0
2	31.967766471228263	31.0	31.0	34.0	30.0	34.0
3	32.018277475191475	33.0	31.0	34.0	30.0	34.0
4	35.73829480190657	37.0	35.0	37.0	33.0	37.0
5	35.71230946333735	37.0	35.0	37.0	33.0	37.0
6	35.759939782563926	37.0	35.0	37.0	33.0	37.0
7	36.102054036311934	37.0	35.0	37.0	35.0	37.0
8	36.03490907025131	37.0	35.0	37.0	35.0	37.0
9	37.79664622335209	39.0	38.0	39.0	35.0	39.0
10	37.27645105321337	39.0	37.0	39.0	34.0	39.0
11	37.06203374290973	39.0	37.0	39.0	33.0	39.0
12	36.758615819543365	39.0	35.0	39.0	33.0	39.0
13	36.6998241291077	39.0	35.0	39.0	33.0	39.0
14	37.82916853070876	40.0	36.0	41.0	33.0	41.0
15	37.88181381355792	40.0	36.0	41.0	33.0	41.0
16	37.907780215826484	40.0	36.0	41.0	33.0	41.0
17	37.83675015208217	40.0	36.0	41.0	33.0	41.0
18	37.71304008893749	39.0	36.0	41.0	33.0	41.0
19	37.637282262130476	39.0	36.0	41.0	33.0	41.0
20	37.4839723940297	39.0	35.0	41.0	33.0	41.0
21	37.42485957549548	39.0	35.0	41.0	32.0	41.0
22	37.33321261441668	39.0	35.0	41.0	32.0	41.0
23	37.222781632103974	39.0	35.0	41.0	32.0	41.0
24	37.12120179232085	39.0	35.0	41.0	32.0	41.0
25	37.00275049766965	39.0	35.0	41.0	32.0	41.0
26	36.86059884472786	39.0	35.0	41.0	31.0	41.0
27	36.773033445452015	39.0	35.0	41.0	31.0	41.0
28	36.78020872537393	39.0	35.0	41.0	31.0	41.0
29	36.77093861719243	39.0	35.0	41.0	31.0	41.0
30	36.67992760021051	39.0	35.0	41.0	31.0	41.0
31	36.547704880910395	39.0	35.0	41.0	31.0	41.0
32	36.39255187954619	39.0	35.0	41.0	30.0	41.0
33	36.202515845344074	39.0	35.0	41.0	30.0	41.0
34	35.94833940478473	39.0	35.0	41.0	29.0	41.0
35	35.80054867931138	39.0	35.0	41.0	28.0	41.0
36	35.670612518314954	39.0	35.0	41.0	27.0	41.0
37	35.60192582177627	38.0	35.0	41.0	27.0	41.0
38	35.471891034213954	38.0	35.0	41.0	26.0	41.0
39	35.39130602876582	38.0	35.0	41.0	26.0	41.0
40	35.26920318413895	38.0	35.0	41.0	25.0	41.0
41	35.19533188628436	38.0	35.0	40.0	25.0	41.0
42	35.10669500799664	38.0	34.0	40.0	24.0	41.0
43	34.99474438755664	38.0	34.0	40.0	24.0	41.0
44	34.847149337190025	38.0	34.0	40.0	24.0	41.0
45	34.739741850881366	37.0	34.0	40.0	23.0	41.0
46	34.66929881245724	37.0	34.0	40.0	23.0	41.0
47	34.610510278071686	37.0	34.0	40.0	23.0	41.0
48	34.50137801037867	37.0	34.0	40.0	23.0	41.0
49	34.40826900908706	37.0	34.0	40.0	23.0	41.0
50	34.29528714927407	36.0	34.0	40.0	23.0	41.0
51	33.930802812356255	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	3.0
13	7.0
14	13.0
15	36.0
16	106.0
17	267.0
18	519.0
19	1071.0
20	1837.0
21	2892.0
22	4515.0
23	6811.0
24	10342.0
25	15568.0
26	20667.0
27	23568.0
28	23705.0
29	25061.0
30	28102.0
31	33328.0
32	39910.0
33	50985.0
34	83643.0
35	119764.0
36	96683.0
37	126029.0
38	188006.0
39	363753.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.337667379144975	24.01919825281066	26.405487739928844	15.237646628115515
2	35.817144768807495	24.70776948525612	25.70634373961955	13.768742006316833
3	29.74900722498771	25.477687909250935	31.113683291949627	13.659621573811728
4	25.42364055114103	28.169404145629624	31.062160773926607	15.34479452930274
5	23.041059422900457	32.102710494734524	29.84234740694978	15.013882675415239
6	21.83126651501846	40.653712658995886	27.486829408390516	10.028191417595139
7	86.71444926531099	3.171041346623461	8.612466240126494	1.5020431479390597
8	86.30061219481982	2.778744318123539	8.484093901958882	2.4365495850977625
9	80.81523930355442	4.597497094461369	11.170602655658364	3.4166609463258446
10	39.89057974273458	26.260230533680186	18.792937075461946	15.056252648123294
11	31.47757586947208	27.025888290028384	25.06156270243103	16.43497313806851
12	26.97902094591556	23.339779565680164	29.762578240454722	19.918621247949552
13	23.541530068872905	26.499695835670785	31.853382536154527	18.105391559301786
14	22.095191205350766	27.228980114517277	32.03856377627707	18.637264903854877
15	22.000272998334395	23.782099988401516	35.57191967536868	18.645707337895402
16	23.941559420138915	23.201071163406862	34.28567145360567	18.571697962848557
17	23.453791875853614	23.691600251537192	30.524606539178023	22.33000133343117
18	24.664531598768193	23.20170237342858	31.623306483237034	20.51045954456619
19	26.143535580914417	26.064160920682937	28.11385766371813	19.678445834684517
20	27.740339133364422	25.130048989787813	27.56699308114915	19.56261879569862
21	26.605502415561848	26.131621491754427	28.39143227077016	18.871443821913562
22	25.4634856837622	23.457894740994803	28.44918798775768	22.629431587485314
23	23.47201806523082	26.813249413960943	28.130979235557323	21.58375328525091
24	22.972888740554534	25.476504390460207	31.670489432360714	19.88011743662454
25	23.861001241116707	25.035525289035014	30.0798401776225	21.023633292225778
26	21.937151996162243	27.233950893438337	29.033688467871805	21.795208642527616
27	22.69231588589932	25.39247455631853	29.13894273899387	22.776266818788283
28	21.65973519161564	26.760780080905345	30.849127391595598	20.730357335883422
29	22.60370977910016	25.034736276507864	29.871146364190825	22.49040758020115
30	23.733575717981676	25.116004566804506	29.71878804519779	21.431631670016024
31	25.398313249019456	25.243824596203112	27.544269520367177	21.813592634410256
32	25.25771121668099	25.02077075477727	27.245628278840183	22.475889749701555
33	24.876302561055763	25.121527654494567	27.047349430766914	22.954820353682756
34	22.17551268061483	27.614886141547267	28.172796899496372	22.036804278341528
35	22.528832490273448	27.675166698621673	28.40792263258764	21.38807817851724
36	24.79629669080256	28.539372119611144	25.91566876307295	20.748662426513345
37	23.162725154587278	28.675003373028552	26.955823977617293	21.206447494766874
38	22.93438492922952	29.409337332048818	26.88599636896435	20.770281369757306
39	23.213300857577714	27.76574533673871	26.610236490724766	22.41071731495881
40	24.127450771535898	26.78058429533686	27.282790768869035	21.809174164258206
41	21.069159315042445	26.882840318855745	27.652522039092414	24.3954783270094
42	23.19807291580368	26.940674937095977	26.934047231867904	22.927204915232437
43	22.543350320773044	25.640697897360514	28.20609322814218	23.60985855372426
44	22.63724281150412	26.892387370434278	27.734973848179788	22.735395969881814
45	21.983545932758773	26.82918746700941	27.258883689296336	23.92838291093548
46	23.944320963983944	26.300706876323076	27.04608701072347	22.70888514896951
47	22.720641435624074	26.086726678959483	28.394746123384202	22.797885762032244
48	23.1607526232694	25.495440691111853	28.704512441544033	22.639294244074712
49	23.60772821990095	25.413856795804346	27.79904166538452	23.179373318910184
50	22.283528495581926	26.390969909429252	28.374310698930966	22.951190896057856
51	22.000983109608832	26.72630023346881	27.43349216155505	23.83922449536731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	651.0
1	1101.5
2	1552.0
3	9021.0
4	16490.0
5	11674.5
6	6859.0
7	6861.0
8	6863.0
9	7303.5
10	7744.0
11	7772.0
12	7800.0
13	7845.5
14	7891.0
15	7862.5
16	7834.0
17	7405.0
18	6976.0
19	7018.5
20	7061.0
21	6990.0
22	6919.0
23	7231.5
24	7544.0
25	7923.0
26	9436.5
27	10571.0
28	11998.5
29	13426.0
30	16075.5
31	18725.0
32	20082.5
33	21440.0
34	24432.0
35	27424.0
36	31109.0
37	34794.0
38	38256.5
39	41719.0
40	49978.5
41	58238.0
42	65694.0
43	73150.0
44	81137.0
45	89124.0
46	94646.0
47	100168.0
48	98545.0
49	96922.0
50	94940.5
51	92959.0
52	89375.5
53	85792.0
54	79837.5
55	73883.0
56	69491.0
57	65099.0
58	61202.0
59	57305.0
60	53771.0
61	50237.0
62	46280.5
63	42324.0
64	37579.0
65	32834.0
66	28702.0
67	24570.0
68	21349.0
69	18128.0
70	15619.5
71	13111.0
72	11143.0
73	9175.0
74	8026.5
75	5678.0
76	4478.0
77	3467.5
78	2457.0
79	1717.0
80	977.0
81	782.0
82	587.0
83	417.0
84	247.0
85	170.0
86	93.0
87	77.0
88	61.0
89	37.5
90	14.0
91	11.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1267407.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.115540722684194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08943668421918	22.7421734354587
2	6.729007817421509	4.187534335324785
3	3.192778227345029	2.9803506285436114
4	2.160708167003724	2.6892641184096275
5	1.6278103925839553	2.53251002796273
6	1.3532730094693908	2.5264692861032527
7	1.1334769497834793	2.4688123732448206
8	0.9635310953134569	2.3984632827038697
9	0.854707167652856	2.3935208112965256
>10	8.798946071918149	51.567806816592864
>50	0.07563193592303857	1.5265155675938802
>100	0.01992609319203554	1.147179614740051
>500	5.10925449470866E-4	0.10079155512954932
>1k	0.0	0.0
>5k	2.55462724735433E-4	0.7386081468957195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9292	0.7331504402295395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044815911542227554	0.0
2	0.0	0.0	0.0	0.11527473021689165	0.0
3	0.0	0.0	0.0	0.19457048919565695	0.0
4	0.0	0.0	0.0	0.3527675008896116	0.0
5	0.0	0.0	0.0	0.6063561271162302	0.0
6	0.0	0.0	0.0	1.308735078786846	0.0
7	0.0	0.0	0.0	1.6760204101760523	0.0
8	0.0	0.0	0.0	2.6798810484714064	0.0
9	0.0	0.0	0.0	3.281345297919295	0.0
10	0.0	0.0	0.0	4.0252263085181	0.0
11	0.0	0.0	0.0	4.524907941963394	0.0
12	0.0	0.0	0.0	4.940480840014297	0.0
13	0.0	0.0	0.0	5.190913416132308	0.0
14	0.0	0.0	0.0	5.33766974618256	0.0
15	0.0	0.0	0.0	5.454759205211901	0.0
16	0.0	0.0	0.0	5.637336703994849	0.0
17	0.0	0.0	0.0	5.828119933060178	0.0
18	0.0	0.0	0.0	6.078947015441764	0.0
19	0.0	0.0	0.0	6.239116558453598	0.0
20	0.0	0.0	0.0	6.4083597455276795	0.0
21	0.0	0.0	0.0	6.608295519907969	0.0
22	0.0	0.0	0.0	6.806653269233956	0.0
23	0.0	0.0	0.0	7.038780754722043	0.0
24	0.0	0.0	0.0	7.2346136639611425	0.0
25	0.0	0.0	0.0	7.407959716176414	0.0
26	0.0	0.0	0.0	7.57104860553871	0.0
27	0.0	0.0	0.0	7.743447842721399	0.0
28	0.0	0.0	0.0	7.916557191178525	0.0
29	0.0	0.0	0.0	8.105762395189549	0.0
30	0.0	0.0	0.0	8.358956515152592	0.0
31	0.0	0.0	0.0	8.561890537136058	0.0
32	0.0	0.0	0.0	8.790309663746532	0.0
33	0.0	0.0	0.0	9.004447663615554	0.0
34	0.0	0.0	0.0	9.212983674541801	0.0
35	0.0	0.0	0.0	9.439903677350685	0.0
36	0.0	0.0	0.0	9.658460147371759	0.0
37	0.0	0.0	0.0	9.870546714670189	0.0
38	0.0	0.0	0.0	10.129263922323295	0.0
39	0.0	0.0	0.0	10.455520602300602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATA	20	7.033561E-4	45.0	27
CGTTTTT	6960	0.0	42.21983	1
TCGATAG	50	1.0822987E-9	40.5	1
GGGCGAT	3265	0.0	39.831547	7
GACGGGA	495	0.0	39.545452	4
GGCGATT	840	0.0	38.839287	8
AGGGCGA	1555	0.0	38.778137	6
TACGGGA	210	0.0	38.57143	4
TCGTTAG	35	6.2487998E-6	38.571426	1
TTACGGG	240	0.0	38.437496	3
GACACGA	1315	0.0	38.15589	25
ACGGGAT	230	0.0	38.152172	5
TCAAGCG	1340	0.0	38.11567	17
CACGACC	1325	0.0	38.037735	27
ACACGAC	1325	0.0	37.867924	26
GCGAGAC	1345	0.0	37.80669	21
TAGGGCG	560	0.0	37.767857	5
ACGACCA	1350	0.0	37.5	28
CGTTCGA	30	1.13998816E-4	37.499996	14
TAATGCG	30	1.13998816E-4	37.499996	1
>>END_MODULE