FastQCFastQC Report
Thu 26 May 2016
SRR1546987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546987_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3399928
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223360.6569550884606968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.035238E-445.015
TACGTAG900.042.5000041
CGTTTTT129700.041.270241
CGAATAT3350.040.9701514
GGGCGAT81200.038.709987
ATAACGG2700.038.3333322
CACGACC38000.038.30920827
AACACGT35050.038.2596341
TAGCATA18950.038.2321930
TCGTTAG1300.038.0769271
GGCGATA17500.038.0571448
ATAGGGC17150.037.7842564
ACACGAC38550.037.7042826
TCAAGCG38850.037.702717
AGACACG38800.037.6353124
AGGGCGA40550.037.6202246
ACGACCA38650.037.6067328
TAAGGGA28100.037.473314
AAACACG38100.037.4409540
TAGGGTA12750.037.4117665
Produced by FastQC (version 0.11.5)