##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546987_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3399928
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.580308759479614	31.0	31.0	33.0	30.0	34.0
2	31.963714525719368	31.0	31.0	34.0	30.0	34.0
3	32.062138374694996	33.0	31.0	34.0	30.0	34.0
4	35.75357625220299	37.0	35.0	37.0	35.0	37.0
5	35.70585288864941	37.0	35.0	37.0	33.0	37.0
6	35.75436097470299	37.0	35.0	37.0	33.0	37.0
7	36.09157488040923	37.0	35.0	37.0	35.0	37.0
8	36.01881981030186	37.0	35.0	37.0	35.0	37.0
9	37.78729137793506	39.0	38.0	39.0	35.0	39.0
10	37.30052077573407	39.0	37.0	39.0	34.0	39.0
11	37.03996143447743	39.0	37.0	39.0	33.0	39.0
12	36.61667864731253	39.0	35.0	39.0	33.0	39.0
13	36.50417744140464	39.0	35.0	39.0	32.0	39.0
14	37.56978088947766	40.0	36.0	41.0	32.0	41.0
15	37.66027221752931	40.0	36.0	41.0	33.0	41.0
16	37.72634655792711	40.0	35.0	41.0	33.0	41.0
17	37.62513941471702	40.0	35.0	41.0	33.0	41.0
18	37.54664539954964	39.0	36.0	41.0	32.0	41.0
19	37.49509401375558	39.0	36.0	41.0	32.0	41.0
20	37.37026048786915	39.0	35.0	41.0	32.0	41.0
21	37.30486792661492	39.0	35.0	41.0	32.0	41.0
22	37.218540216145755	39.0	35.0	41.0	32.0	41.0
23	37.12919538296105	39.0	35.0	41.0	32.0	41.0
24	37.01261056116483	39.0	35.0	41.0	32.0	41.0
25	36.87900773192844	39.0	35.0	41.0	31.0	41.0
26	36.72389062356615	38.0	35.0	41.0	31.0	41.0
27	36.628959201488975	38.0	35.0	41.0	31.0	41.0
28	36.64663898764915	38.0	35.0	41.0	31.0	41.0
29	36.62871654929163	38.0	35.0	41.0	31.0	41.0
30	36.528909141605354	38.0	35.0	41.0	31.0	41.0
31	36.409629262737326	38.0	35.0	41.0	30.0	41.0
32	36.31201131318075	38.0	35.0	41.0	30.0	41.0
33	36.16562527206459	38.0	35.0	41.0	30.0	41.0
34	35.942938497521126	38.0	35.0	41.0	29.0	41.0
35	35.8224080039342	38.0	35.0	41.0	29.0	41.0
36	35.716269579826395	38.0	35.0	41.0	29.0	41.0
37	35.650168474155926	38.0	35.0	41.0	28.0	41.0
38	35.52178134360492	38.0	35.0	40.0	28.0	41.0
39	35.40320706791438	38.0	35.0	40.0	27.0	41.0
40	35.24748141725354	38.0	34.0	40.0	27.0	41.0
41	35.16174960175627	37.0	34.0	40.0	26.0	41.0
42	35.06104717511665	37.0	34.0	40.0	26.0	41.0
43	34.91039692605255	37.0	34.0	40.0	26.0	41.0
44	34.73573910976938	37.0	34.0	40.0	25.0	41.0
45	34.612753858317	36.0	34.0	40.0	25.0	41.0
46	34.530402408521596	36.0	34.0	40.0	24.0	41.0
47	34.43762838507168	36.0	34.0	40.0	24.0	41.0
48	34.32286713130396	35.0	34.0	40.0	24.0	41.0
49	34.21337893037735	35.0	33.0	40.0	24.0	41.0
50	34.073423907800404	35.0	33.0	40.0	24.0	41.0
51	33.699922763070276	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	4.0
12	6.0
13	15.0
14	29.0
15	108.0
16	272.0
17	705.0
18	1476.0
19	2761.0
20	4845.0
21	7811.0
22	11773.0
23	17323.0
24	25434.0
25	36104.0
26	48510.0
27	56711.0
28	62193.0
29	69574.0
30	79750.0
31	94040.0
32	114383.0
33	147820.0
34	253967.0
35	360681.0
36	273545.0
37	356185.0
38	512326.0
39	860984.0
40	589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.748471144094815	23.69897244882833	27.4678463779233	14.084710029153557
2	32.541306757084264	25.37259612556501	27.875060883642245	14.21103623370848
3	30.220934090368974	25.323595087895978	30.52008748420555	13.9353833375295
4	25.543776221143506	28.433425649013742	30.307994757536044	15.714803372306708
5	23.48746796990995	32.25824193924107	29.15314677251989	15.101143318329093
6	22.443681160306923	40.897542536194884	25.849106216366934	10.809670087131257
7	87.97145116014221	3.320482080797005	7.046796285097802	1.661270473962978
8	87.51261791426171	3.020475727721293	6.7969380528058245	2.669968305211169
9	82.18976990100967	4.614480071342688	9.643733632006326	3.5520163956413193
10	45.032865401855574	24.255131285133096	15.87336555362349	14.838637759387845
11	35.46478043064442	25.226975394773067	22.1988524462871	17.109391728295424
12	30.473233550828134	21.172654244442825	27.532435980997246	20.821676223731796
13	24.833967072243883	25.59945387078785	29.662186963959236	19.90439209300903
14	22.191499349397986	26.357205211404477	30.68506156600963	20.766233873187904
15	22.124203806668845	22.07732046090388	35.587253612429436	20.211222119997835
16	25.68633806362958	20.262664385834054	33.169143581864084	20.881853968672278
17	24.145452491935124	21.627016807414744	28.78140360619401	25.446127094456116
18	26.50664955257876	21.455395526022905	29.45118249562932	22.586772425769016
19	27.53661254003026	25.28765315030201	25.50998138784115	21.66575292182658
20	29.896927229047204	24.26351381558668	24.42392897731952	21.415629978046592
21	27.861531185366278	25.0420008894306	26.132847519123935	20.963620406079187
22	26.582121739048592	21.861904134440493	26.47044290349678	25.085531223014137
23	26.153965613389463	24.96841109576438	25.389331774084628	23.48829151676153
24	25.80242875731486	23.139313538404345	29.565626095611435	21.49263160866936
25	25.47153939730488	23.290169674181335	27.292842671962465	23.945448256551316
26	23.321111505890713	25.490539799666344	26.857862872390236	24.3304858220527
27	23.555616471878228	23.827210458574417	27.336196531220665	25.280976538326694
28	22.401503796550987	26.03755138344106	28.55019282761282	23.010751992395136
29	24.53669607121092	23.493379859808798	27.881649258454882	24.088274810525398
30	26.64527013513227	23.371524338162455	27.749881762202023	22.23332376450325
31	28.12915449974235	24.260278452955475	24.814996082269978	22.7955709650322
32	28.641165342324896	23.635588753644196	24.622933191526407	23.1003127125045
33	27.582966462819215	23.780121226096554	24.23354259266667	24.403369718417565
34	24.188453402542642	25.947255353642785	26.782037737269732	23.082253506544845
35	24.29998517615667	25.657896284862503	27.002601231555488	23.039517307425335
36	26.89330479939575	26.688329870514906	24.081362899449633	22.337002430639707
37	24.85890877689175	26.96989465659273	25.29653569134405	22.874660875171475
38	24.84696734754383	26.772449298926333	25.49312808977131	22.887455263758525
39	24.999029391210637	25.937961039174944	24.684581555844712	24.378428013769703
40	26.079816984359667	23.97986074999235	26.399941410523986	23.540380855123992
41	22.18644041873828	24.435164509366082	26.77394933069171	26.604445741203932
42	24.817554959987387	24.7564360186451	25.40586153589135	25.020147485476162
43	23.55143991284521	23.358700537187847	27.400697897131938	25.689161652835
44	23.992890437679858	24.55034341903711	26.4527366461878	25.00402949709523
45	23.052899943763517	24.466488702113693	26.04805160579871	26.43255974832408
46	25.120502551818745	24.32719163464638	26.14296538044335	24.409340433091526
47	23.60555870594907	24.72743540451445	27.900502598878564	23.76650329065792
48	24.56325545717439	23.358229938986945	28.097359708793835	23.98115489504484
49	24.55172580125226	23.68423684266255	27.108956424959587	24.6550809311256
50	23.443555275288183	24.60007976639505	27.72182234447318	24.234542613843587
51	23.57732281389488	24.413016981536078	26.64885844641416	25.360801758154878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1735.0
1	3224.5
2	4714.0
3	20880.5
4	37047.0
5	25192.5
6	13338.0
7	13314.0
8	13290.0
9	14000.0
10	14710.0
11	15095.5
12	15481.0
13	15390.5
14	15300.0
15	15035.0
16	14770.0
17	14183.0
18	13596.0
19	13424.0
20	13252.0
21	12861.5
22	12471.0
23	12973.0
24	13475.0
25	14963.0
26	18263.5
27	20076.0
28	21778.5
29	23481.0
30	27381.0
31	31281.0
32	38337.5
33	45394.0
34	51384.5
35	57375.0
36	63719.0
37	70063.0
38	80143.5
39	90224.0
40	104373.0
41	118522.0
42	133591.5
43	148661.0
44	166712.0
45	184763.0
46	202771.0
47	220779.0
48	238694.0
49	256609.0
50	259271.5
51	261934.0
52	249645.5
53	237357.0
54	226133.5
55	214910.0
56	211316.0
57	207722.0
58	203149.5
59	198577.0
60	194397.0
61	190217.0
62	177641.5
63	165066.0
64	149316.0
65	133566.0
66	116243.0
67	98920.0
68	84629.0
69	70338.0
70	62636.0
71	54934.0
72	47163.0
73	39392.0
74	33469.0
75	22060.0
76	16574.0
77	12422.5
78	8271.0
79	6171.0
80	4071.0
81	3152.5
82	2234.0
83	1538.5
84	843.0
85	580.5
86	318.0
87	241.5
88	165.0
89	114.0
90	63.0
91	43.5
92	24.0
93	23.0
94	22.0
95	14.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3399928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.709964093687073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67927529569982	16.864142774932766
2	7.4266408361658325	3.224642117797407
3	2.5048239752800012	1.6313891569300605
4	1.34837155622271	1.1709239228217598
5	0.8993088680865303	0.976198161764647
6	0.63921538919282	0.8326405888504997
7	0.5271739708000353	0.8011449584036643
8	0.43090774736302284	0.7483993378354246
9	0.35727668790864425	0.6980817659407298
>10	5.774282740883084	31.878960466505013
>50	2.1915079997429547	32.69813554961743
>100	0.21475047856670582	6.413940438910429
>500	0.003988705713815344	0.5812890358353122
>1k	0.002338206797753819	0.8131536972243901
>5k	0.0	0.0
>10k+	1.3754157633845995E-4	0.6669580266305809
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22336	0.6569550884606968	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07326625740309795	0.0
2	0.0	0.0	0.0	0.20676908452178988	0.0
3	0.0	0.0	0.0	0.3162419910068684	0.0
4	0.0	0.0	0.0	0.5399231983736126	0.0
5	0.0	0.0	0.0	0.8977837177728469	0.0
6	0.0	0.0	0.0	1.6365052436404535	0.0
7	0.0	0.0	0.0	2.0036600775075235	0.0
8	0.0	0.0	0.0	2.9624744994599888	0.0
9	0.0	0.0	0.0	3.4598085606518727	0.0
10	0.0	0.0	0.0	4.196000621189625	0.0
11	0.0	0.0	0.0	4.775630542764435	0.0
12	0.0	0.0	0.0	5.229669569473236	0.0
13	0.0	0.0	0.0	5.469468765220911	0.0
14	0.0	0.0	0.0	5.599795054483507	0.0
15	0.0	0.0	0.0	5.725533011287298	0.0
16	5.882477511288474E-5	0.0	0.0	5.945067072008584	0.0
17	5.882477511288474E-5	0.0	0.0	6.179572037996099	0.0
18	5.882477511288474E-5	0.0	0.0	6.479990164497601	0.0
19	5.882477511288474E-5	0.0	0.0	6.642228894258937	0.0
20	5.882477511288474E-5	0.0	0.0	6.816261991430407	0.0
21	5.882477511288474E-5	0.0	0.0	7.0313841940182265	0.0
22	5.882477511288474E-5	0.0	0.0	7.253712431557374	0.0
23	5.882477511288474E-5	0.0	0.0	7.5043353859258195	0.0
24	5.882477511288474E-5	0.0	0.0	7.715545740968632	0.0
25	5.882477511288474E-5	0.0	0.0	7.897431945617672	0.0
26	5.882477511288474E-5	0.0	0.0	8.072259177253166	0.0
27	5.882477511288474E-5	0.0	0.0	8.250498245845206	0.0
28	5.882477511288474E-5	0.0	0.0	8.432443275269359	0.0
29	5.882477511288474E-5	0.0	0.0	8.631771025739368	0.0
30	5.882477511288474E-5	0.0	0.0	8.901070846206155	0.0
31	5.882477511288474E-5	0.0	0.0	9.10275158768068	0.0
32	5.882477511288474E-5	0.0	0.0	9.331491725707133	0.0
33	5.882477511288474E-5	0.0	0.0	9.542849142687727	0.0
34	5.882477511288474E-5	0.0	0.0	9.756441901122612	0.0
35	5.882477511288474E-5	0.0	0.0	9.996505808358295	0.0
36	5.882477511288474E-5	0.0	0.0	10.211951547209235	0.0
37	5.882477511288474E-5	0.0	0.0	10.433662124609697	0.0
38	8.823716266932712E-5	0.0	0.0	10.696314745488728	0.0
39	8.823716266932712E-5	0.0	0.0	11.051410500457656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	20	7.035238E-4	45.0	15
TACGTAG	90	0.0	42.500004	1
CGTTTTT	12970	0.0	41.27024	1
CGAATAT	335	0.0	40.97015	14
GGGCGAT	8120	0.0	38.70998	7
ATAACGG	270	0.0	38.333332	2
CACGACC	3800	0.0	38.309208	27
AACACGT	3505	0.0	38.25963	41
TAGCATA	1895	0.0	38.23219	30
TCGTTAG	130	0.0	38.076927	1
GGCGATA	1750	0.0	38.057144	8
ATAGGGC	1715	0.0	37.784256	4
ACACGAC	3855	0.0	37.70428	26
TCAAGCG	3885	0.0	37.7027	17
AGACACG	3880	0.0	37.63531	24
AGGGCGA	4055	0.0	37.620224	6
ACGACCA	3865	0.0	37.60673	28
TAAGGGA	2810	0.0	37.47331	4
AAACACG	3810	0.0	37.44095	40
TAGGGTA	1275	0.0	37.411766	5
>>END_MODULE