FastQCFastQC Report
Thu 26 May 2016
SRR1546986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546986_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3134070
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337811.0778636086622189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT220300.043.100321
CGTTCGA850.042.3529414
CGATCGA650.041.53846410
ACGGGTA6250.039.9600035
CGGGTAT5550.038.108116
TACGGGA9750.038.0769274
GGGCGAT83050.038.064427
AACGGGC4850.038.0412374
TACGGGT5450.037.981654
ACGGGAT10150.037.9064035
GGCGATA16100.037.593178
AGGGCGA41550.037.5270736
AACACGT28700.037.00348341
ATAGGGC15750.036.8571434
ACACGAC31550.036.79873326
CACGACC31750.036.7795327
GCGAGAC31950.036.6901421
TCAAGCG31900.036.4655217
GACACGA31850.036.38147425
ACGACCA31850.036.38147428
Produced by FastQC (version 0.11.5)