##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546986_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3134070
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59630480493416	31.0	31.0	33.0	30.0	34.0
2	31.981505199309524	31.0	31.0	34.0	30.0	34.0
3	32.012625116860825	33.0	31.0	34.0	30.0	34.0
4	35.74777780968517	37.0	35.0	37.0	33.0	37.0
5	35.718938632512995	37.0	35.0	37.0	33.0	37.0
6	35.77387550373795	37.0	35.0	37.0	33.0	37.0
7	36.13544209286965	37.0	35.0	37.0	35.0	37.0
8	36.07550597146841	37.0	35.0	37.0	35.0	37.0
9	37.838421286059344	39.0	38.0	39.0	35.0	39.0
10	37.23497177791179	39.0	37.0	39.0	34.0	39.0
11	37.122922270402384	39.0	37.0	39.0	33.0	39.0
12	36.9493980670502	39.0	37.0	39.0	33.0	39.0
13	36.92887618974688	39.0	37.0	39.0	33.0	39.0
14	38.05502876451388	40.0	37.0	41.0	33.0	41.0
15	38.097914213785906	40.0	37.0	41.0	33.0	41.0
16	38.121940160877074	40.0	37.0	41.0	33.0	41.0
17	38.04640196294275	40.0	37.0	41.0	33.0	41.0
18	37.91533979777095	39.0	37.0	41.0	33.0	41.0
19	37.81876218463531	40.0	37.0	41.0	33.0	41.0
20	37.63855593525352	40.0	35.0	41.0	33.0	41.0
21	37.594392594932465	40.0	35.0	41.0	33.0	41.0
22	37.51412508335806	39.0	35.0	41.0	33.0	41.0
23	37.40421879536832	39.0	35.0	41.0	32.0	41.0
24	37.30607165762092	39.0	35.0	41.0	32.0	41.0
25	37.21673319357895	39.0	35.0	41.0	32.0	41.0
26	37.09151263373186	39.0	35.0	41.0	32.0	41.0
27	37.01283347213049	39.0	35.0	41.0	32.0	41.0
28	37.02035819238243	39.0	35.0	41.0	32.0	41.0
29	36.99996968797761	39.0	35.0	41.0	31.0	41.0
30	36.88886591556666	39.0	35.0	41.0	31.0	41.0
31	36.746702211501336	39.0	35.0	41.0	31.0	41.0
32	36.560570121279994	39.0	35.0	41.0	30.0	41.0
33	36.3285277610264	39.0	35.0	41.0	30.0	41.0
34	36.02832738260473	39.0	35.0	41.0	28.0	41.0
35	35.86368747347698	39.0	35.0	41.0	27.0	41.0
36	35.73525511555262	39.0	35.0	41.0	26.0	41.0
37	35.66650202452402	39.0	35.0	41.0	26.0	41.0
38	35.5433943721742	39.0	35.0	41.0	25.0	41.0
39	35.44936615965821	39.0	35.0	41.0	25.0	41.0
40	35.33829461371316	39.0	35.0	41.0	24.0	41.0
41	35.261370039597075	38.0	35.0	41.0	24.0	41.0
42	35.17999693689037	38.0	35.0	41.0	24.0	41.0
43	35.075361750056636	38.0	34.0	41.0	23.0	41.0
44	34.92267371181882	38.0	34.0	40.0	23.0	41.0
45	34.82293152354606	38.0	34.0	40.0	23.0	41.0
46	34.768171738346624	38.0	34.0	40.0	23.0	41.0
47	34.7159415711838	38.0	34.0	40.0	23.0	41.0
48	34.61561930652474	38.0	34.0	40.0	23.0	41.0
49	34.51889172864678	37.0	34.0	40.0	23.0	41.0
50	34.425676516478575	37.0	34.0	40.0	23.0	41.0
51	34.06452408529484	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	4.0
13	11.0
14	23.0
15	73.0
16	202.0
17	550.0
18	1185.0
19	2270.0
20	4076.0
21	6715.0
22	10137.0
23	15485.0
24	23898.0
25	38047.0
26	54724.0
27	63478.0
28	63388.0
29	63577.0
30	68204.0
31	78433.0
32	94451.0
33	119179.0
34	183625.0
35	252403.0
36	231722.0
37	303154.0
38	472203.0
39	982231.0
40	616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.219423943945095	23.47832052251545	26.429530929430424	16.872724604109035
2	35.188429103370375	24.732982990169333	26.436103852179432	13.642484054280853
3	28.797410396066457	24.89628502235113	32.87590896182919	13.430395619753227
4	24.51853340863478	27.76960948542949	32.70258800856394	15.009269097371789
5	22.797065796233014	31.32686889571707	31.23006186843306	14.646003439616855
6	21.086191437970434	40.126831883142366	28.299655081092638	10.48732159779456
7	86.03515556448963	2.530575258370107	9.976133270794845	1.4581359063454231
8	85.673389554158	2.242260064389117	9.747548714610714	2.3368016668421574
9	79.65204989039812	4.215796073476342	12.782867006799464	3.349287029326084
10	31.96128357056479	28.42284952154866	25.389381858095067	14.226485049791485
11	27.764280950967912	26.004524468183543	29.02506963788301	17.206124942965538
12	24.821845076848952	23.618840676819598	31.71246972786185	19.846844518469595
13	23.230368179396123	24.856305060193296	33.77735660020357	18.135970160207016
14	21.321444639079534	25.90452670170098	33.81082745439636	18.96320120482312
15	21.250961210183565	24.240332857913195	36.217282957942864	18.291422973960376
16	24.003930990692613	23.136369002606834	34.60426857089982	18.25543143580073
17	22.87638757270897	23.613544049750004	32.49321170235508	21.01685667518594
18	23.778888155018873	22.72233868420297	33.212531947276226	20.28624121350193
19	24.71093498230735	26.047344188228088	30.152581148474667	19.089139680989895
20	26.238373744045283	25.102885385457245	29.70386749498256	18.954873375514904
21	25.263826270632116	25.562798533536263	30.952339928591254	18.221035267240364
22	24.200735784459184	22.56331224254723	30.979812193090773	22.25613977990281
23	22.039360958753313	26.446027051086922	30.82649717460044	20.688114815559324
24	21.063058578780947	25.573998028123174	33.57436177239181	19.78858162070407
25	22.706991228657945	25.372694292086646	31.39763949114091	20.5226749881145
26	21.12658619622408	26.666985740586526	30.840791686209947	21.365636376979456
27	21.430280753142082	24.923916823810572	31.468186734820858	22.17761568822649
28	20.336846337190938	26.89984588729671	32.045008567134744	20.718299208377605
29	21.51984480244538	24.910611441352618	31.256194022469185	22.313349733732814
30	22.9597296805751	24.53665680728254	31.823730803715293	20.679882708427062
31	23.698928230703206	25.644290012667238	29.707441122884937	20.94934063374462
32	23.271113918961607	25.507854004537233	29.470879718704435	21.750152357796733
33	22.978491227062573	25.56330905180803	28.729288114177407	22.728911606951982
34	20.937088195222188	27.667729182819784	30.328199433962865	21.066983187995163
35	21.25169508019923	27.8195445538868	29.558688861448534	21.37007150446544
36	22.877536238820447	28.594160309118816	28.15192385619977	20.376379595860975
37	22.588231915687906	28.362353106344145	28.006809037449703	21.04260594051824
38	22.396915193342842	27.633045847731548	29.556519158793513	20.413519800132097
39	22.96065499494268	26.75080645933243	27.84641057793859	22.442127967786295
40	23.82850414955633	25.883723082126437	29.553838937866733	20.733933830450503
41	20.98070560006637	26.485560309756963	29.447810674298914	23.085923415877758
42	22.83369548223237	26.826044089634244	28.26267441378144	22.077586014351944
43	22.215521669905268	25.580188062168364	29.673364028244425	22.530926239681946
44	22.468738732702207	26.78928677406695	28.667196329373628	22.07477816385722
45	21.505007865172125	26.149607379541617	28.76875755806348	23.57662719722278
46	22.797608221896766	26.191788951746453	28.640170768361905	22.370432057994876
47	21.994754424757584	26.606010714502226	29.885420555380065	21.513814305360125
48	22.40310522738803	25.949675661360466	29.401544955919938	22.245674155331567
49	22.483767114327378	25.856155095450962	29.187446355697222	22.472631434524438
50	21.47581260150539	26.01447957448302	30.222936947802697	22.28677087620889
51	21.49045809442674	26.006343189526714	29.179884303796662	23.323314412249886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1354.0
1	2340.5
2	3327.0
3	30149.0
4	56971.0
5	39585.0
6	22199.0
7	21937.5
8	21676.0
9	22150.0
10	22624.0
11	22590.0
12	22556.0
13	22138.0
14	21720.0
15	20965.5
16	20211.0
17	19424.5
18	18638.0
19	18371.5
20	18105.0
21	17830.0
22	17555.0
23	18391.0
24	19227.0
25	21199.5
26	26786.5
27	30401.0
28	34713.0
29	39025.0
30	45450.0
31	51875.0
32	60107.0
33	68339.0
34	78187.0
35	88035.0
36	94059.5
37	100084.0
38	110024.5
39	119965.0
40	132319.5
41	144674.0
42	156255.0
43	167836.0
44	184849.5
45	201863.0
46	219236.5
47	236610.0
48	248341.0
49	260072.0
50	256121.0
51	252170.0
52	236234.5
53	220299.0
54	200538.5
55	180778.0
56	167489.0
57	154200.0
58	143278.0
59	132356.0
60	120895.0
61	109434.0
62	98329.5
63	87225.0
64	75844.0
65	64463.0
66	53930.0
67	43397.0
68	36307.5
69	29218.0
70	24247.5
71	19277.0
72	16775.5
73	14274.0
74	13509.0
75	10651.5
76	8559.0
77	6397.5
78	4236.0
79	3013.0
80	1790.0
81	1297.5
82	805.0
83	616.0
84	427.0
85	319.5
86	212.0
87	128.5
88	45.0
89	36.0
90	27.0
91	18.0
92	9.0
93	8.0
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3134070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.106175502334317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31374685896502	19.661538932385202
2	6.66567322873646	3.4803047030121084
3	2.710107467445395	2.122516235259488
4	1.5924159442852688	1.662875604569066
5	1.1774877428812587	1.5369850833752823
6	0.9198203895767013	1.440779551254893
7	0.7630761061386857	1.3944699123946092
8	0.6954415755470906	1.452425585828179
9	0.5932662720382107	1.3939122075700625
>10	9.07345366871123	54.61910074335804
>50	0.4440675035239335	7.082892520406263
>100	0.04699923917825767	2.098620429540799
>500	0.002962668648306833	0.514208345545188
>1k	0.0013578897971372938	0.45072185515048924
>5k	0.0	0.0
>10k+	1.2344452701248127E-4	1.0886482903502557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33781	1.0778636086622189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.572217595650385E-5	0.0	0.0	0.06579304227410364	0.0
2	9.572217595650385E-5	0.0	0.0	0.19230585149661622	0.0
3	9.572217595650385E-5	0.0	0.0	0.31026747966701446	0.0
4	9.572217595650385E-5	0.0	0.0	0.5001483693727325	0.0
5	9.572217595650385E-5	0.0	0.0	0.8676258028697509	0.0
6	9.572217595650385E-5	0.0	0.0	1.6456237416522286	0.0
7	9.572217595650385E-5	0.0	0.0	2.0673118341326133	0.0
8	9.572217595650385E-5	0.0	0.0	3.0409339931781996	0.0
9	9.572217595650385E-5	0.0	0.0	3.5873799883218944	0.0
10	9.572217595650385E-5	0.0	0.0	4.363591113153184	0.0
11	9.572217595650385E-5	0.0	0.0	4.8670259439004235	0.0
12	9.572217595650385E-5	0.0	0.0	5.261528938409161	0.0
13	9.572217595650385E-5	0.0	0.0	5.496590695166349	0.0
14	9.572217595650385E-5	0.0	0.0	5.639918699965221	0.0
15	9.572217595650385E-5	0.0	0.0	5.769239359682458	0.0
16	9.572217595650385E-5	0.0	0.0	5.981455423778026	0.0
17	9.572217595650385E-5	0.0	0.0	6.205126241596391	0.0
18	9.572217595650385E-5	0.0	0.0	6.505534337139885	0.0
19	9.572217595650385E-5	0.0	0.0	6.665645630123131	0.0
20	9.572217595650385E-5	0.0	0.0	6.842029693018982	0.0
21	9.572217595650385E-5	0.0	0.0	7.048757685693045	0.0
22	9.572217595650385E-5	0.0	0.0	7.270577874776249	0.0
23	9.572217595650385E-5	0.0	0.0	7.519200273127275	0.0
24	9.572217595650385E-5	0.0	0.0	7.722897063562716	0.0
25	9.572217595650385E-5	0.0	0.0	7.913767082419985	0.0
26	9.572217595650385E-5	0.0	0.0	8.09273564406666	0.0
27	9.572217595650385E-5	0.0	0.0	8.281850756364728	0.0
28	9.572217595650385E-5	0.0	0.0	8.474667126133111	0.0
29	9.572217595650385E-5	0.0	0.0	8.685734524117203	0.0
30	9.572217595650385E-5	0.0	0.0	8.967572517525134	0.0
31	9.572217595650385E-5	0.0	0.0	9.190222298799963	0.0
32	9.572217595650385E-5	0.0	0.0	9.433101366593599	0.0
33	9.572217595650385E-5	0.0	0.0	9.665100013720178	0.0
34	9.572217595650385E-5	0.0	0.0	9.89020666417789	0.0
35	9.572217595650385E-5	0.0	0.0	10.131554177156222	0.0
36	9.572217595650385E-5	0.0	0.0	10.355767420638339	0.0
37	9.572217595650385E-5	0.0	0.0	10.593381768754368	0.0
38	9.572217595650385E-5	0.0	0.0	10.860414732281027	0.0
39	9.572217595650385E-5	0.0	0.0	11.232008219344175	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22030	0.0	43.10032	1
CGTTCGA	85	0.0	42.35294	14
CGATCGA	65	0.0	41.538464	10
ACGGGTA	625	0.0	39.960003	5
CGGGTAT	555	0.0	38.10811	6
TACGGGA	975	0.0	38.076927	4
GGGCGAT	8305	0.0	38.06442	7
AACGGGC	485	0.0	38.041237	4
TACGGGT	545	0.0	37.98165	4
ACGGGAT	1015	0.0	37.906403	5
GGCGATA	1610	0.0	37.59317	8
AGGGCGA	4155	0.0	37.527073	6
AACACGT	2870	0.0	37.003483	41
ATAGGGC	1575	0.0	36.857143	4
ACACGAC	3155	0.0	36.798733	26
CACGACC	3175	0.0	36.77953	27
GCGAGAC	3195	0.0	36.69014	21
TCAAGCG	3190	0.0	36.46552	17
GACACGA	3185	0.0	36.381474	25
ACGACCA	3185	0.0	36.381474	28
>>END_MODULE