FastQCFastQC Report
Thu 26 May 2016
SRR1546985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546985_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences980605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.6576552230510756No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT10590.10799455438224362No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT10170.1037114842367722No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG207.0327776E-445.0000041
TCGTAAG406.8139343E-945.0000041
ACGTAAG351.2116834E-745.0000041
TAGTACG351.2116834E-745.0000041
ATTCGCG302.165174E-644.9999961
CGTTTTT49400.042.267211
CGGTCTA501.0804797E-940.531
ATAACGC501.0804797E-940.511
AGTACGG950.040.2631572
CGGATAC451.927765E-840.014
TGATTCG451.927765E-840.015
GTACGGG2550.039.7058833
CGAGGGA4950.039.5454524
TAGGGCG4850.039.432995
CGACAGG750.039.02
CGTTCGG700.038.5714345
CGTAAGG1400.038.571432
ATAGGGC5900.038.516954
GGTACCT3350.038.2835858
CGGGTAT1300.038.076926
Produced by FastQC (version 0.11.5)