##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546985_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	980605
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495869386756134	31.0	31.0	33.0	30.0	34.0
2	31.857904049030957	31.0	31.0	34.0	30.0	34.0
3	31.912326573900806	33.0	31.0	34.0	30.0	34.0
4	35.63735551011875	37.0	35.0	37.0	33.0	37.0
5	35.599620642358545	37.0	35.0	37.0	33.0	37.0
6	35.65686081551695	37.0	35.0	37.0	33.0	37.0
7	36.05005991199311	37.0	35.0	37.0	35.0	37.0
8	35.978590767944276	37.0	35.0	37.0	35.0	37.0
9	37.73051024622554	39.0	38.0	39.0	35.0	39.0
10	37.162539452684825	39.0	37.0	39.0	33.0	39.0
11	36.93201951856252	39.0	37.0	39.0	33.0	39.0
12	36.507056358064666	39.0	35.0	39.0	32.0	39.0
13	36.362146837921486	39.0	35.0	39.0	32.0	39.0
14	37.45550960886391	40.0	35.0	41.0	32.0	41.0
15	37.56590472208484	40.0	35.0	41.0	32.0	41.0
16	37.64607665675782	40.0	35.0	41.0	33.0	41.0
17	37.613752734281384	40.0	35.0	41.0	33.0	41.0
18	37.50171679728331	39.0	36.0	41.0	33.0	41.0
19	37.41413107214424	39.0	35.0	41.0	32.0	41.0
20	37.22722604922471	39.0	35.0	41.0	32.0	41.0
21	37.16093432115888	39.0	35.0	41.0	32.0	41.0
22	37.06015775975036	39.0	35.0	41.0	32.0	41.0
23	36.96237526832925	39.0	35.0	41.0	32.0	41.0
24	36.84262164684047	38.0	35.0	41.0	31.0	41.0
25	36.73064587678015	38.0	35.0	41.0	31.0	41.0
26	36.55326456626266	38.0	35.0	41.0	31.0	41.0
27	36.464864038017346	38.0	35.0	40.0	31.0	41.0
28	36.481190693500444	38.0	35.0	41.0	31.0	41.0
29	36.48342706798354	38.0	35.0	41.0	31.0	41.0
30	36.41112884392798	38.0	35.0	40.0	31.0	41.0
31	36.307131821681516	38.0	35.0	40.0	30.0	41.0
32	36.13440682027932	38.0	35.0	41.0	30.0	41.0
33	35.96035508691063	38.0	35.0	41.0	30.0	41.0
34	35.75198780344787	38.0	35.0	41.0	29.0	41.0
35	35.61020492451089	38.0	35.0	41.0	28.0	41.0
36	35.478279225580124	38.0	35.0	41.0	27.0	41.0
37	35.39806955909872	38.0	35.0	41.0	27.0	41.0
38	35.288553495036226	38.0	35.0	40.0	26.0	41.0
39	35.195614951993925	38.0	35.0	40.0	26.0	41.0
40	35.07520051396842	38.0	34.0	40.0	25.0	41.0
41	35.01490304454903	38.0	34.0	40.0	25.0	41.0
42	34.91843504775113	38.0	34.0	40.0	24.0	41.0
43	34.801447065842005	37.0	34.0	40.0	24.0	41.0
44	34.656512051233676	37.0	34.0	40.0	24.0	41.0
45	34.567355867041265	37.0	34.0	40.0	23.0	41.0
46	34.492520433813816	37.0	34.0	40.0	23.0	41.0
47	34.42606146205659	37.0	34.0	40.0	23.0	41.0
48	34.31732042973471	36.0	34.0	40.0	23.0	41.0
49	34.231078772798426	36.0	34.0	40.0	23.0	41.0
50	34.11903059845707	36.0	34.0	40.0	23.0	41.0
51	33.74249774373983	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	5.0
14	10.0
15	41.0
16	77.0
17	206.0
18	444.0
19	878.0
20	1492.0
21	2472.0
22	3811.0
23	5503.0
24	8444.0
25	12396.0
26	16173.0
27	18029.0
28	18851.0
29	20030.0
30	23057.0
31	27286.0
32	33001.0
33	42502.0
34	70535.0
35	115379.0
36	73990.0
37	93687.0
38	135824.0
39	256248.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.76425777963604	25.10225829972313	27.34128420719862	13.792199713442214
2	33.32585495688886	25.6872033081618	27.390947425313968	13.595994309635378
3	28.719209059713137	25.483757476251906	32.22479999592088	13.572233468114073
4	24.68159962472147	28.715333901010094	31.40520393022675	15.197862544041687
5	22.97520408319354	32.201854977284434	30.747854640757495	14.075086298764539
6	20.74046124586352	41.43360476440564	27.23145405132546	10.594479938405373
7	86.09460486128462	3.331514728152518	8.706563804997936	1.867316605564932
8	85.5720703035371	2.946752260084336	8.142830191565412	3.338347244813151
9	79.99724659776362	4.797446474370414	11.502082897802888	3.703224030063073
10	42.72862161624712	23.607772752535425	18.296051927126623	15.367553704090842
11	34.195114240698345	25.779493272010644	23.912176666445713	16.113215820845294
12	30.45160895569572	21.70853707660067	28.6049938558339	19.234860111869715
13	21.993871130577553	30.20910560317355	30.458645428077563	17.338377838171333
14	19.35590783240958	31.871140775337675	29.854528581844882	18.91842281040786
15	18.00612886942245	24.898302578510208	39.75759862533844	17.337969926728906
16	19.509282534761706	21.756160737503887	39.05782654585689	19.676730181877513
17	19.91413464136936	23.86363520479704	29.194017978696824	27.02821217513678
18	21.57158080980619	24.44358329806599	32.387148750006375	21.597687142121448
19	25.342620117172558	28.017907312322492	26.27694127604897	20.362531294455973
20	26.93582023342732	24.660796141157753	27.118870493215923	21.284513132199
21	22.952361042417692	28.814558359380175	27.876464019661327	20.356616578540798
22	23.192722859867125	25.37209171888783	26.412571830655562	25.022613590589483
23	21.59554560704871	28.975785356998994	25.868315988598876	23.56035304735342
24	21.222000703647236	25.291733164729933	33.201136033367156	20.285130098255667
25	21.21445434196236	26.19556294328501	30.500456350926214	22.089526363826415
26	19.938915261496728	29.83239938609328	28.582864660082297	21.645820692327696
27	21.51539100861203	28.32822594214796	28.25847308549314	21.89790996374687
28	20.065775720091167	27.015057031118545	32.33677168686678	20.582395561923505
29	19.815624028023514	24.850067050443347	31.605182514876017	23.729126406657112
30	21.2135365412169	27.169961401379762	29.713595178486752	21.902906878916585
31	23.989679840506625	26.019039266575227	27.054216529591425	22.937064363326723
32	24.699547728188207	26.023934203884338	27.480382009065828	21.79613605886162
33	23.537306050856362	26.35882949811596	28.1268196674502	21.977044783577483
34	19.961758302272575	26.971920396082012	28.992509726138454	24.073811575506955
35	20.11441915960045	24.827937854691744	30.710122832333102	24.347520153374703
36	23.974179205694444	26.65160793591711	27.104491614870412	22.26972124351803
37	20.858653586306414	28.292533690935702	29.492201243110124	21.356611479647768
38	21.001932480458493	28.709113251513095	27.4009412556534	22.888013012375012
39	20.490207575935266	28.169038501741273	27.81854059483686	23.5222133274866
40	22.711591313525833	25.43705161609415	27.739405775006247	24.111951295373775
41	18.10810673002891	24.681191713279045	29.29742352935178	27.913278027340265
42	21.654080899036817	26.156505422672737	27.022807348524637	25.166606329765806
43	20.96471056133713	25.98977162058117	29.54992071221338	23.495597105868317
44	21.198851729289572	27.950499946461623	27.97681023449809	22.873838089750713
45	19.91066739410874	28.660163878421997	26.357605763788683	25.071562963680584
46	21.936151661474295	28.2262480815415	26.69494852667486	23.14265173030935
47	21.557303909321288	25.660689064404117	28.54421505091245	24.23779197536215
48	21.74494317283718	23.89647207591232	29.78294012369915	24.57564462755136
49	21.017229159549462	23.902998658991134	29.797420979905265	25.282351201554143
50	19.733429872374707	26.69617226100214	29.650368904910746	23.92002896171241
51	19.133902029869315	27.354643306938065	27.551970467211568	25.95948419598105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	632.0
1	883.5
2	1135.0
3	5878.0
4	10621.0
5	7696.0
6	4771.0
7	4819.5
8	4868.0
9	5241.0
10	5614.0
11	5913.0
12	6212.0
13	6125.5
14	6039.0
15	6075.5
16	6112.0
17	5823.5
18	5535.0
19	5569.5
20	5604.0
21	5619.5
22	5635.0
23	5807.5
24	5980.0
25	5843.5
26	6514.5
27	7322.0
28	8530.5
29	9739.0
30	11400.5
31	13062.0
32	14875.5
33	16689.0
34	19321.5
35	21954.0
36	23945.0
37	25936.0
38	30170.0
39	34404.0
40	43099.5
41	51795.0
42	61219.5
43	70644.0
44	80096.0
45	89548.0
46	92995.0
47	96442.0
48	90924.5
49	85407.0
50	76454.0
51	67501.0
52	62041.5
53	56582.0
54	52233.0
55	47884.0
56	44648.0
57	41412.0
58	39451.0
59	37490.0
60	35485.0
61	33480.0
62	29957.0
63	26434.0
64	24092.5
65	21751.0
66	18256.5
67	14762.0
68	12987.0
69	11212.0
70	9893.0
71	8574.0
72	7206.5
73	5839.0
74	5231.5
75	3676.5
76	2729.0
77	2018.5
78	1308.0
79	1107.0
80	906.0
81	656.0
82	406.0
83	297.5
84	189.0
85	122.5
86	56.0
87	47.0
88	38.0
89	24.0
90	10.0
91	7.0
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	980605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.93328590516863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4633019144269	27.060476729350714
2	8.035870818628494	5.614387456083014
3	3.1434490276421645	3.2943301083284418
4	1.8371632657715031	2.5671259847067684
5	1.2966374754168242	2.264790382204599
6	0.9152265685831973	1.9183122832993953
7	0.699330138956992	1.7100929780400138
8	0.5573630334723318	1.5576417761008832
9	0.43996521707073766	1.383248764463549
>10	5.231610948835702	42.4442630083402
>50	0.3544879574228591	7.530560042931282
>100	0.0226518367870633	1.283863573091303
>500	0.0020592578896991482	0.4948213483045798
>1k	5.883593970568994E-4	0.21334353459611788
>5k	2.941796985284497E-4	0.662742030159135
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6449	0.6576552230510756	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1059	0.10799455438224362	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	1017	0.1037114842367722	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11921211904895447	0.0
2	0.0	0.0	0.0	0.3432574788013522	0.0
3	0.0	0.0	0.0	0.5279393843596555	0.0
4	0.0	0.0	0.0	0.8904706788156291	0.0
5	0.0	0.0	0.0	1.4980547723089317	0.0
6	0.0	0.0	0.0	2.730253262016816	0.0
7	0.0	0.0	0.0	3.306326196582722	0.0
8	0.0	0.0	0.0	4.958673471989231	0.0
9	0.0	0.0	0.0	5.734826969065016	0.0
10	0.0	0.0	0.0	6.885341192427124	0.0
11	0.0	0.0	0.0	7.789680860285232	0.0
12	0.0	0.0	0.0	8.515559272082031	0.0
13	0.0	0.0	0.0	8.93703376996854	0.0
14	0.0	0.0	0.0	9.122531498411695	0.0
15	0.0	0.0	0.0	9.309762850485159	0.0
16	0.0	0.0	0.0	9.676577215086605	0.0
17	0.0	0.0	0.0	10.087649971191254	0.0
18	0.0	0.0	0.0	10.634659215484318	0.0
19	0.0	0.0	0.0	10.918973490855135	0.0
20	0.0	0.0	0.0	11.215831043080547	0.0
21	0.0	0.0	0.0	11.618643592476074	0.0
22	0.0	0.0	0.0	12.028594592114052	0.0
23	0.0	0.0	0.0	12.555412220007037	0.0
24	0.0	0.0	0.0	12.950474451996472	0.0
25	0.0	0.0	0.0	13.294037864379643	0.0
26	0.0	0.0	0.0	13.603948582762682	0.0
27	0.0	0.0	0.0	13.903865470806288	0.0
28	0.0	0.0	0.0	14.23937263220155	0.0
29	0.0	0.0	0.0	14.609348310481794	0.0
30	0.0	0.0	0.0	15.020625022307657	0.0
31	0.0	0.0	0.0	15.395393660036406	0.0
32	0.0	0.0	0.0	15.756497264443889	0.0
33	0.0	0.0	0.0	16.12545316411807	0.0
34	0.0	0.0	0.0	16.465957240683046	0.0
35	0.0	0.0	0.0	16.85877595973914	0.0
36	0.0	0.0	0.0	17.220389453449656	0.0
37	0.0	0.0	0.0	17.59556600262083	0.0
38	0.0	0.0	0.0	17.987772854513285	0.0
39	0.0	0.0	0.0	18.357646554932924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATCG	20	7.0327776E-4	45.000004	1
TCGTAAG	40	6.8139343E-9	45.000004	1
ACGTAAG	35	1.2116834E-7	45.000004	1
TAGTACG	35	1.2116834E-7	45.000004	1
ATTCGCG	30	2.165174E-6	44.999996	1
CGTTTTT	4940	0.0	42.26721	1
CGGTCTA	50	1.0804797E-9	40.5	31
ATAACGC	50	1.0804797E-9	40.5	11
AGTACGG	95	0.0	40.263157	2
CGGATAC	45	1.927765E-8	40.0	14
TGATTCG	45	1.927765E-8	40.0	15
GTACGGG	255	0.0	39.705883	3
CGAGGGA	495	0.0	39.545452	4
TAGGGCG	485	0.0	39.43299	5
CGACAGG	75	0.0	39.0	2
CGTTCGG	70	0.0	38.57143	45
CGTAAGG	140	0.0	38.57143	2
ATAGGGC	590	0.0	38.51695	4
GGTACCT	335	0.0	38.283585	8
CGGGTAT	130	0.0	38.07692	6
>>END_MODULE