FastQCFastQC Report
Thu 26 May 2016
SRR1546984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546984_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2731775
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT522431.912419580675568No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT38900.14239825754317248No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC29430.10773215217212252No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT28330.10370546622617163No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG207.034994E-445.030
CGTTTTT371650.044.225081
GCGCGAC2200.042.9545449
CGACGGT900.042.528
CCGACGG501.0822987E-940.52
GGCGCGA2400.040.3125048
TCGAGCG403.4605546E-739.3751
TACGGGA8200.039.23784
CGTTAGG3900.039.2307662
GTTTTTT421250.039.135312
TAGGGCG11650.039.0128755
GCTACGG2550.038.823532
GGACCGA14900.038.657728
AGGGCGA27650.038.571436
GCGATCG2050.038.414639
TGGGCGA17250.038.0869566
TAATGCG659.094947E-1238.0769271
GGCGATA11350.038.0616728
GACCGAT14450.037.837379
GGGCGAT56450.037.8255127
Produced by FastQC (version 0.11.5)