FastQCFastQC Report
Thu 26 May 2016
SRR1546983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546983_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3043410
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152660.5016083932168193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG1200.041.2499961
CGTTTTT101250.041.2444461
CGTTAGG4250.040.7647062
TCGTTAG1450.040.3448261
CGTAAGG6050.039.4214862
GACCGAT20750.038.7108429
GGGCGAT77300.038.334417
AGGGCGA38950.038.12586
TAATGCG1500.037.51
CTTAACG601.5643309E-1037.4999961
TACGGGA4750.037.421054
TAGGGTC20150.037.1836245
TAAGGGT25350.037.1005944
AACACGT24750.037.09090841
CACGACC26450.037.0037827
GCGAGAC27300.036.67582321
CGAGGGT14250.036.6315774
GGCGATG38600.036.6062168
ACACGAC26750.036.58878326
TGGGCGA23000.036.4891326
Produced by FastQC (version 0.11.5)