##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546983_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3043410
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606021863633227	31.0	31.0	33.0	30.0	34.0
2	31.99726392434802	31.0	31.0	34.0	30.0	34.0
3	32.11077508452689	33.0	31.0	34.0	30.0	34.0
4	35.795934165952005	37.0	35.0	37.0	35.0	37.0
5	35.75133912289176	37.0	35.0	37.0	33.0	37.0
6	35.804946753805766	37.0	35.0	37.0	35.0	37.0
7	36.13506921512383	37.0	35.0	37.0	35.0	37.0
8	36.07139228694129	37.0	35.0	37.0	35.0	37.0
9	37.848066149483635	39.0	38.0	39.0	35.0	39.0
10	37.22073299358286	39.0	37.0	39.0	34.0	39.0
11	37.096034711064235	39.0	37.0	39.0	33.0	39.0
12	36.816525870651674	39.0	35.0	39.0	33.0	39.0
13	36.73637268721598	39.0	35.0	39.0	33.0	39.0
14	37.82474724075954	40.0	36.0	41.0	33.0	41.0
15	37.90125352811484	40.0	36.0	41.0	33.0	41.0
16	37.944587814326695	40.0	36.0	41.0	33.0	41.0
17	37.88186540755271	40.0	36.0	41.0	33.0	41.0
18	37.812418307096316	40.0	36.0	41.0	33.0	41.0
19	37.773082167700046	40.0	36.0	41.0	33.0	41.0
20	37.65502380553392	40.0	35.0	41.0	33.0	41.0
21	37.601845298530264	40.0	35.0	41.0	33.0	41.0
22	37.52140658011901	39.0	35.0	41.0	33.0	41.0
23	37.41587561321018	39.0	35.0	41.0	32.0	41.0
24	37.32840596567666	39.0	35.0	41.0	32.0	41.0
25	37.20468126213688	39.0	35.0	41.0	32.0	41.0
26	37.08148787051367	39.0	35.0	41.0	32.0	41.0
27	36.99724453819893	39.0	35.0	41.0	31.0	41.0
28	37.03135627470502	39.0	35.0	41.0	32.0	41.0
29	37.0217069011405	39.0	35.0	41.0	32.0	41.0
30	36.95257260769991	39.0	35.0	41.0	31.0	41.0
31	36.84403415905185	39.0	35.0	41.0	31.0	41.0
32	36.75251214920106	39.0	35.0	41.0	31.0	41.0
33	36.63044939722219	39.0	35.0	41.0	31.0	41.0
34	36.4572886991894	39.0	35.0	41.0	30.0	41.0
35	36.366630522998875	39.0	35.0	41.0	30.0	41.0
36	36.260101990858935	39.0	35.0	41.0	30.0	41.0
37	36.19542914033929	39.0	35.0	41.0	30.0	41.0
38	36.08108437574957	39.0	35.0	41.0	30.0	41.0
39	35.98179016300794	39.0	35.0	41.0	29.0	41.0
40	35.86519923375424	38.0	35.0	41.0	29.0	41.0
41	35.78910136984501	38.0	35.0	41.0	28.0	41.0
42	35.710969603175386	38.0	35.0	41.0	28.0	41.0
43	35.59789085269484	38.0	35.0	41.0	28.0	41.0
44	35.460083261867446	38.0	35.0	40.0	27.0	41.0
45	35.3630861435035	38.0	34.0	40.0	27.0	41.0
46	35.306649777716444	38.0	34.0	40.0	27.0	41.0
47	35.237249006870584	38.0	34.0	40.0	27.0	41.0
48	35.13490163993678	37.0	34.0	40.0	26.0	41.0
49	35.031623080689094	37.0	34.0	40.0	26.0	41.0
50	34.9240716827506	37.0	34.0	40.0	26.0	41.0
51	34.54434532317368	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	8.0
14	29.0
15	82.0
16	191.0
17	467.0
18	1080.0
19	2078.0
20	3598.0
21	5778.0
22	8928.0
23	13091.0
24	19579.0
25	28256.0
26	38107.0
27	44893.0
28	49397.0
29	54770.0
30	63278.0
31	75986.0
32	93252.0
33	119185.0
34	197658.0
35	294470.0
36	224205.0
37	295681.0
38	463188.0
39	945622.0
40	549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.87998659398504	24.306748022777082	27.867917894729928	12.945347488507958
2	32.51898364006164	25.40567981310438	27.97365455196638	14.101681994867599
3	29.778110737626545	25.34676563460066	30.65830762204238	14.216816005730415
4	25.427431729540217	28.35947177672414	30.57859440561738	15.634502088118262
5	23.658724917115997	32.503737583828666	28.771641021091472	15.065896477963864
6	22.402469598246704	41.27281569029477	25.47096842029171	10.853746291166816
7	89.29559277258076	2.9768581952480933	6.269776336412117	1.4577726957590333
8	89.2714093730388	2.315527648262968	6.066320344613444	2.346742634084793
9	83.58147604167694	3.871348257382344	9.389730598243418	3.157445102697303
10	36.88421868890488	23.07441324041125	24.94553806421087	15.095830006473001
11	32.47682698026227	25.752856171202698	23.55873838884672	18.21157845968831
12	28.639158049687687	23.97333911632018	26.9732306853168	20.414272148675337
13	24.435156617084125	27.048606661606556	29.523987895157077	18.992248826152245
14	22.039291452679723	28.16689174314338	29.90320068607253	19.89061611810436
15	22.77244932493486	23.781974824292487	34.50668822143582	18.93888762933683
16	24.850545933673086	22.517373603950833	33.14571483960426	19.486365622771824
17	23.774286080416378	23.094095110418905	29.312350291285107	23.819268517879618
18	25.102697303353803	23.387187398345937	30.505847059712625	21.00426823858764
19	26.593492168324346	25.55327740922189	27.199325756306248	20.65390466614751
20	28.02425568687755	25.65217962745736	26.21703943931314	20.106525246351953
21	26.53148934911826	26.286369565717404	28.03585451845134	19.146286566712998
22	25.166605879589017	23.15941657548605	28.37751075274117	23.296466792183768
23	23.662273568135742	26.887274471727434	27.38155555774608	22.068896402390738
24	23.082529136724926	25.471494146368713	31.024377261032853	20.42159945587351
25	23.54638382603724	25.45522949586155	29.35628127659435	21.64210540150686
26	22.133396420462574	27.638044167562043	28.045186156318074	22.183373255657305
27	22.49654170814974	26.3101258128218	28.53089133570567	22.662441143322788
28	21.627779365908637	27.588363053285626	29.77981934737679	21.00403823342895
29	22.528545283087066	25.73954872987866	29.547448421343166	22.184457565691115
30	24.41839909837978	25.660558386809534	29.158213977084912	20.762828537725774
31	25.89430934379528	25.979509826148956	26.918325168150197	21.20785566190556
32	26.59930801305115	25.825767806506516	26.679809818591643	20.895114361850688
33	25.63752501306101	25.710009495927267	26.744934136379918	21.90753135463181
34	22.743107238262343	28.04278753109177	28.414377293890738	20.799727936755154
35	22.766107754131056	26.99159824013196	28.58993694572864	21.652357060008345
36	25.853466999188413	27.960314252762526	26.009311923138846	20.176906824910215
37	23.228220975813315	27.90948311269267	27.592963156459366	21.269332755034647
38	23.936965443367804	27.884543981915023	27.05806973099254	21.120420843724638
39	23.588737633115485	27.07403866058139	26.73376245724368	22.603461249059443
40	24.77839002960495	25.54279574556172	27.46869465500869	22.210119569824638
41	21.44926250488761	25.741290197508714	27.92666778383458	24.882779513769094
42	23.820714264591363	26.081402111447353	27.09559342973835	23.002290194222926
43	22.927407086130362	24.71944299322142	28.774893951192904	23.578255969455313
44	22.642857846954566	26.810255601447057	27.859604851137377	22.687281700460996
45	22.118150364229596	26.424701239727806	27.502571129095326	23.954577266947272
46	23.749018370840602	26.042826960547544	27.415596321231778	22.792558347380076
47	23.11147692883969	25.809174577201233	28.523038302430496	22.55631019152858
48	23.623698417235932	25.601381345267317	28.68929917428148	22.085621063215275
49	23.385347357076437	25.20363671013764	28.641753822192868	22.769262110593054
50	22.49535882447649	26.324418990540217	28.5370357592306	22.643186425752692
51	22.18800621671086	26.671693922277974	27.437085374629117	23.703214486382052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1156.0
1	2139.0
2	3122.0
3	15423.5
4	27725.0
5	19490.0
6	11255.0
7	11193.0
8	11131.0
9	11782.5
10	12434.0
11	12524.5
12	12615.0
13	12841.5
14	13068.0
15	12566.5
16	12065.0
17	12103.5
18	12142.0
19	11903.0
20	11664.0
21	11951.0
22	12238.0
23	12743.5
24	13249.0
25	14787.0
26	20087.5
27	23850.0
28	28624.0
29	33398.0
30	39413.5
31	45429.0
32	50856.5
33	56284.0
34	67307.0
35	78330.0
36	83663.0
37	88996.0
38	100142.5
39	111289.0
40	130515.0
41	149741.0
42	163575.0
43	177409.0
44	190395.5
45	203382.0
46	220158.0
47	236934.0
48	246488.0
49	256042.0
50	250282.0
51	244522.0
52	232061.5
53	219601.0
54	205546.0
55	191491.0
56	177246.0
57	163001.0
58	151368.0
59	139735.0
60	129437.0
61	119139.0
62	108988.0
63	98837.0
64	87435.0
65	76033.0
66	63930.0
67	51827.0
68	43260.0
69	34693.0
70	29436.5
71	24180.0
72	20275.5
73	16371.0
74	15202.5
75	12089.0
76	10144.0
77	7323.0
78	4502.0
79	3559.0
80	2616.0
81	1632.5
82	649.0
83	510.0
84	371.0
85	281.5
86	192.0
87	134.5
88	77.0
89	74.5
90	72.0
91	55.5
92	39.0
93	22.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3043410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.2026008135222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5428248566501	16.441095613884187
2	7.090227367201249	3.006625210877571
3	2.397769374387883	1.5251684066410542
4	1.304472874684238	1.1063287053599067
5	0.8945239579620342	0.948311719940046
6	0.6799051753844014	0.8649454814833958
7	0.5299724451019813	0.786575593696453
8	0.4431013732423581	0.7515921229424981
9	0.38803681854909344	0.7404650788181019
>10	6.202889654064432	33.573319301241526
>50	2.335246760556335	33.794100532949464
>100	0.18788306460990412	5.292058761832324
>500	0.0017304526833594215	0.2577646120575387
>1k	0.0012585110424432157	0.40245701463331546
>5k	0.0	0.0
>10k+	1.5731388030540197E-4	0.5091918436426481
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15266	0.5016083932168193	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.571575962489444E-5	0.0	0.0	0.055299811724348676	0.0
2	6.571575962489444E-5	0.0	0.0	0.16511084605754728	0.0
3	6.571575962489444E-5	0.0	0.0	0.25441856338777885	0.0
4	6.571575962489444E-5	0.0	0.0	0.4524201471375858	0.0
5	6.571575962489444E-5	0.0	0.0	0.7669357727023306	0.0
6	6.571575962489444E-5	0.0	0.0	1.4189675397005332	0.0
7	6.571575962489444E-5	0.0	0.0	1.7368346690061478	0.0
8	6.571575962489444E-5	0.0	0.0	2.606812752800313	0.0
9	6.571575962489444E-5	0.0	0.0	3.0326508751696286	0.0
10	6.571575962489444E-5	0.0	0.0	3.6957886055444384	0.0
11	6.571575962489444E-5	0.0	0.0	4.196444120246697	0.0
12	9.857363943734167E-5	0.0	0.0	4.589687225842066	0.0
13	9.857363943734167E-5	0.0	0.0	4.800700529997601	0.0
14	9.857363943734167E-5	0.0	0.0	4.9077843603063664	0.0
15	9.857363943734167E-5	0.0	0.0	5.018811136192626	0.0
16	9.857363943734167E-5	0.0	0.0	5.233044512569782	0.0
17	9.857363943734167E-5	0.0	0.0	5.463181102776162	0.0
18	9.857363943734167E-5	0.0	0.0	5.762089235429995	0.0
19	9.857363943734167E-5	0.0	0.0	5.919445621851804	0.0
20	9.857363943734167E-5	0.0	0.0	6.094512405492523	0.0
21	9.857363943734167E-5	0.0	0.0	6.308285771552304	0.0
22	9.857363943734167E-5	0.0	0.0	6.534085121623442	0.0
23	9.857363943734167E-5	0.0	0.0	6.780420646577359	0.0
24	9.857363943734167E-5	0.0	0.0	6.976516473298044	0.0
25	9.857363943734167E-5	0.0	0.0	7.162262067877808	0.0
26	9.857363943734167E-5	0.0	0.0	7.329442960363539	0.0
27	9.857363943734167E-5	0.0	0.0	7.501519676941325	0.0
28	9.857363943734167E-5	0.0	0.0	7.6758635872261705	0.0
29	9.857363943734167E-5	0.0	0.0	7.869823651759047	0.0
30	9.857363943734167E-5	0.0	0.0	8.144055516673731	0.0
31	9.857363943734167E-5	0.0	0.0	8.35792745637295	0.0
32	9.857363943734167E-5	0.0	0.0	8.583693948564274	0.0
33	9.857363943734167E-5	0.0	0.0	8.795134405157373	0.0
34	9.857363943734167E-5	0.0	0.0	9.011076391284776	0.0
35	9.857363943734167E-5	0.0	0.0	9.250051751160704	0.0
36	9.857363943734167E-5	0.0	0.0	9.462576517787614	0.0
37	9.857363943734167E-5	0.0	0.0	9.681837149776074	0.0
38	9.857363943734167E-5	0.0	0.0	9.939377211746034	0.0
39	9.857363943734167E-5	0.0	0.0	10.311065548184438	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	120	0.0	41.249996	1
CGTTTTT	10125	0.0	41.244446	1
CGTTAGG	425	0.0	40.764706	2
TCGTTAG	145	0.0	40.344826	1
CGTAAGG	605	0.0	39.421486	2
GACCGAT	2075	0.0	38.710842	9
GGGCGAT	7730	0.0	38.33441	7
AGGGCGA	3895	0.0	38.1258	6
TAATGCG	150	0.0	37.5	1
CTTAACG	60	1.5643309E-10	37.499996	1
TACGGGA	475	0.0	37.42105	4
TAGGGTC	2015	0.0	37.183624	5
TAAGGGT	2535	0.0	37.100594	4
AACACGT	2475	0.0	37.090908	41
CACGACC	2645	0.0	37.00378	27
GCGAGAC	2730	0.0	36.675823	21
CGAGGGT	1425	0.0	36.631577	4
GGCGATG	3860	0.0	36.606216	8
ACACGAC	2675	0.0	36.588783	26
TGGGCGA	2300	0.0	36.489132	6
>>END_MODULE