FastQCFastQC Report
Thu 26 May 2016
SRR1546982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546982_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1921705
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160280.8340510119919551No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104600.042.0315481
CGCATCG700.041.78571321
ATAACGC950.040.26315711
CACGACC19800.039.88636427
GCGAGAC19850.039.55919621
ACGACCA20050.039.1645928
GACACGA19950.039.02255625
ACACGAC20050.038.9401526
TCAAGCG20450.038.7286117
CGACCAA20450.038.5085629
AAACACG20050.038.26683440
AACACGT17650.037.98866741
AGGGCGA23750.037.86
CGAGACA20850.037.5539622
AGACACG20800.037.42788324
GACCGAT16300.037.13199
GTCAAGC21300.037.0774616
TAGGGCG9000.037.05
GGGCGAT51650.036.9845127
AGGGATT24900.036.9578326
Produced by FastQC (version 0.11.5)