##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546982_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1921705
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.642532542715973	31.0	31.0	33.0	30.0	34.0
2	32.035783848197305	33.0	31.0	34.0	30.0	34.0
3	32.094099250405236	33.0	31.0	34.0	30.0	34.0
4	35.80277253792856	37.0	35.0	37.0	35.0	37.0
5	35.770318545250184	37.0	35.0	37.0	35.0	37.0
6	35.819417652553334	37.0	35.0	37.0	35.0	37.0
7	36.15685237848681	37.0	35.0	37.0	35.0	37.0
8	36.0988606471857	37.0	35.0	37.0	35.0	37.0
9	37.87218641779045	39.0	38.0	39.0	35.0	39.0
10	37.30050137768284	39.0	37.0	39.0	34.0	39.0
11	37.15732955890732	39.0	37.0	39.0	33.0	39.0
12	36.915307500370766	39.0	35.0	39.0	33.0	39.0
13	36.86803541646611	39.0	37.0	39.0	33.0	39.0
14	38.021712489690145	40.0	37.0	41.0	33.0	41.0
15	38.07382975014375	40.0	37.0	41.0	33.0	41.0
16	38.1055448156715	40.0	37.0	41.0	33.0	41.0
17	38.026308408418565	40.0	36.0	41.0	33.0	41.0
18	37.90611462217146	40.0	36.0	41.0	33.0	41.0
19	37.835602759008275	40.0	36.0	41.0	33.0	41.0
20	37.692127043432784	40.0	35.0	41.0	33.0	41.0
21	37.62949516184846	40.0	35.0	41.0	33.0	41.0
22	37.536539687413	39.0	35.0	41.0	33.0	41.0
23	37.420943901379246	39.0	35.0	41.0	32.0	41.0
24	37.32795668429858	39.0	35.0	41.0	32.0	41.0
25	37.22292495466266	39.0	35.0	41.0	32.0	41.0
26	37.09263024241494	39.0	35.0	41.0	32.0	41.0
27	37.010122261221156	39.0	35.0	41.0	32.0	41.0
28	37.018768749625984	39.0	35.0	41.0	32.0	41.0
29	37.02594675041174	39.0	35.0	41.0	32.0	41.0
30	36.925522908042595	39.0	35.0	41.0	31.0	41.0
31	36.812957764068884	39.0	35.0	41.0	31.0	41.0
32	36.6709859213563	39.0	35.0	41.0	31.0	41.0
33	36.484244980369	39.0	35.0	41.0	30.0	41.0
34	36.26352639973357	39.0	35.0	41.0	30.0	41.0
35	36.12073497232926	39.0	35.0	41.0	29.0	41.0
36	36.0048618284284	39.0	35.0	41.0	29.0	41.0
37	35.92952508319435	39.0	35.0	41.0	28.0	41.0
38	35.81481236714272	39.0	35.0	41.0	28.0	41.0
39	35.709237890310945	39.0	35.0	41.0	27.0	41.0
40	35.60140552269989	39.0	35.0	41.0	27.0	41.0
41	35.52224352853325	38.0	35.0	41.0	26.0	41.0
42	35.425421175466575	38.0	35.0	41.0	26.0	41.0
43	35.31277433320931	38.0	35.0	41.0	26.0	41.0
44	35.17314103881709	38.0	34.0	40.0	25.0	41.0
45	35.07489078708751	38.0	34.0	40.0	24.0	41.0
46	35.017487075279504	38.0	34.0	40.0	24.0	41.0
47	34.959376178966075	38.0	34.0	40.0	24.0	41.0
48	34.86418987305544	38.0	34.0	40.0	24.0	41.0
49	34.765367733340966	37.0	34.0	40.0	24.0	41.0
50	34.66575983306491	37.0	34.0	40.0	24.0	41.0
51	34.31581382158031	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	0.0
13	6.0
14	10.0
15	48.0
16	126.0
17	328.0
18	715.0
19	1353.0
20	2469.0
21	3933.0
22	5912.0
23	8547.0
24	13233.0
25	20739.0
26	29193.0
27	33590.0
28	34846.0
29	36719.0
30	40361.0
31	47385.0
32	57228.0
33	73182.0
34	117352.0
35	169946.0
36	143123.0
37	185273.0
38	289609.0
39	606073.0
40	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.43582651863839	22.57843945870984	26.79989904798083	16.18583497467093
2	34.47063935411522	24.800216474432858	26.859169331401024	13.869974840050892
3	28.878990271659806	25.592897973414235	31.691544748023237	13.836567006902726
4	24.70670576389196	28.82445536645843	31.32816951613281	15.1406693535168
5	23.304357328518165	32.00553674991739	30.0568505571875	14.633255364376947
6	22.214127558600303	40.40953216024312	27.232691802331782	10.143648478824794
7	87.60772334983777	2.7907509217075464	8.206566564587177	1.394959163867503
8	87.41877655519448	2.392042483107449	8.139854972537409	2.049325989160667
9	81.72466637699335	4.444282551172006	10.805248464254399	3.0258026075802475
10	35.0769238774942	30.315943394017292	21.7478749339779	12.859257794510604
11	29.441615648603715	26.450886062116712	27.48767370642216	16.619824582857408
12	26.24185293788589	24.171764136535003	30.38140609510825	19.20497683047086
13	23.582703900962947	26.953304487421327	31.698413648296697	17.765577963319032
14	21.48758524331258	27.96131560255086	31.837456841710875	18.713642312425684
15	21.33766629113209	24.705560947179716	35.813457320452414	18.143315441235778
16	24.021845184354518	23.99041476189113	34.26988013248651	17.71785992126783
17	22.997130152650904	23.78309886272867	31.187096874910562	22.03267410970987
18	24.10474032174553	23.30820807564116	32.13380825881183	20.453243343801468
19	25.74838489778608	26.139599990633318	28.593514613325144	19.518500498255456
20	27.46977293601255	25.545648265472586	28.15421721856372	18.830361579951138
21	26.505420967318084	25.792408304084134	29.09218636575333	18.609984362844454
22	24.76300993128498	23.63822751150671	29.540954516952393	22.057808040255917
23	22.73392638308169	27.234096804660446	29.420540613673797	20.61143619858407
24	22.2532074381864	25.62604562094598	32.56889064658727	19.55185629428034
25	22.747560109382032	26.474458878964253	30.833712770690614	19.944268240963105
26	21.741526404937282	27.951480586250234	29.028597001100586	21.2783960077119
27	21.869329579722173	25.87238936257126	30.16201758334396	22.09626347436261
28	20.652025154745395	27.572390143128107	31.60901387049521	20.166570831631287
29	21.619915647823156	25.228429961934847	31.071262238480934	22.080392151761068
30	22.725496369109724	25.530609536843585	31.034055695333052	20.709838398713643
31	23.926721322991824	26.158177243645618	28.502241499085446	21.41285993427711
32	24.220574958175163	25.770084378195406	28.651223783046824	21.35811688058261
33	22.851998615812523	26.09016472351375	28.3813592616973	22.67647739897643
34	20.875316450755967	27.70393999078943	30.242310864570786	21.178432693883817
35	21.08315272115127	27.72340187489755	29.716787956528183	21.47665744742299
36	23.629381200548472	28.043586294462468	27.65840750791615	20.66862499707291
37	22.467340200499038	28.16681020239839	28.093750081308006	21.27209951579457
38	22.785755357872304	27.983431379946456	28.427724338543115	20.803088923638125
39	22.636304739801375	27.138400534941624	27.942894460908413	22.28240026434859
40	24.08871288777414	26.419039342667062	28.17123335787751	21.321014411681293
41	21.037308015538287	26.793342370447075	28.781732888242473	23.387616725772165
42	23.02694742429249	27.53601619395277	27.33322752451599	22.103808857238754
43	22.09834495929396	26.53169971457638	28.824091106595446	22.545864219534213
44	22.158447836686694	27.574627739429307	28.65361749071788	21.613306933166122
45	21.650149216451016	27.401135970401285	27.820711295438166	23.128003517709534
46	22.930418560601133	26.87993214359124	27.813374061055157	22.37627523475247
47	21.829833403149806	26.936808719340377	29.25266885396042	21.9806890235494
48	22.57646204802506	26.232174032955108	29.163997595884904	22.027366323134924
49	22.623191384733868	26.189607666109005	28.870300072071416	22.316900877085715
50	21.298586411545998	27.359662383144133	29.41767857189319	21.92407263341668
51	21.511158060160117	27.15812260466617	28.456865127582017	22.873854207591695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	750.0
1	1411.0
2	2072.0
3	14899.0
4	27726.0
5	19466.0
6	11206.0
7	11000.5
8	10795.0
9	11168.0
10	11541.0
11	11696.5
12	11852.0
13	11726.5
14	11601.0
15	11403.0
16	11205.0
17	10893.0
18	10581.0
19	10223.0
20	9865.0
21	10455.5
22	11046.0
23	11363.5
24	11681.0
25	12934.5
26	16594.5
27	19001.0
28	21459.0
29	23917.0
30	28826.0
31	33735.0
32	38173.0
33	42611.0
34	48697.5
35	54784.0
36	60801.5
37	66819.0
38	72591.5
39	78364.0
40	86929.0
41	95494.0
42	105201.0
43	114908.0
44	120101.5
45	125295.0
46	134981.0
47	144667.0
48	147393.0
49	150119.0
50	149503.0
51	148887.0
52	139057.0
53	129227.0
54	119586.5
55	109946.0
56	101505.0
57	93064.0
58	86139.5
59	79215.0
60	73009.0
61	66803.0
62	61093.5
63	55384.0
64	48011.0
65	40638.0
66	34438.0
67	28238.0
68	24479.5
69	20721.0
70	17195.0
71	13669.0
72	12334.5
73	11000.0
74	10177.5
75	7415.5
76	5476.0
77	3961.0
78	2446.0
79	1683.5
80	921.0
81	682.5
82	444.0
83	368.0
84	292.0
85	185.5
86	79.0
87	61.0
88	43.0
89	31.0
90	19.0
91	15.0
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1921705.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.269859274787304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65571066164365	19.349251538658297
2	6.573338476093384	3.4536135346503607
3	2.7879259426670777	2.1971526654717857
4	1.8274500286648727	1.9202742033892894
5	1.3797216495142102	1.8122546785557854
6	1.0912596662367733	1.7200342718574835
7	0.9017780223828952	1.6582707221566257
8	0.8377952753094757	1.7607011186769292
9	0.7417838725401923	1.7537902149543854
>10	9.874943291600713	57.08516800824918
>50	0.30041580162308185	4.784501494628576
>100	0.02547065560497187	1.2910098406979016
>500	0.0022061014421752975	0.3695356151499987
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.0055467656139068E-4	0.8444420929033618
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16028	0.8340510119919551	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.203712328375063E-5	0.0	0.0	0.04792619054433433	0.0
2	5.203712328375063E-5	0.0	0.0	0.13560874327745415	0.0
3	5.203712328375063E-5	0.0	0.0	0.21709887833980762	0.0
4	5.203712328375063E-5	0.0	0.0	0.3696717238077645	0.0
5	5.203712328375063E-5	0.0	0.0	0.6055560036530061	0.0
6	5.203712328375063E-5	0.0	0.0	1.2122568240182545	0.0
7	5.203712328375063E-5	0.0	0.0	1.5461270070067987	0.0
8	5.203712328375063E-5	0.0	0.0	2.3212199583182644	0.0
9	5.203712328375063E-5	0.0	0.0	2.7589042022578907	0.0
10	5.203712328375063E-5	0.0	0.0	3.287861560437216	0.0
11	5.203712328375063E-5	0.0	0.0	3.671427196161742	0.0
12	5.203712328375063E-5	0.0	0.0	3.996034771205778	0.0
13	5.203712328375063E-5	0.0	0.0	4.192891208588207	0.0
14	5.203712328375063E-5	0.0	0.0	4.32209938570176	0.0
15	5.203712328375063E-5	0.0	0.0	4.433198643912567	0.0
16	5.203712328375063E-5	0.0	0.0	4.58780093718859	0.0
17	5.203712328375063E-5	0.0	0.0	4.756349179504658	0.0
18	5.203712328375063E-5	0.0	0.0	4.976934545104478	0.0
19	5.203712328375063E-5	0.0	0.0	5.117226629477469	0.0
20	5.203712328375063E-5	0.0	0.0	5.262097980699431	0.0
21	5.203712328375063E-5	0.0	0.0	5.428356589591014	0.0
22	5.203712328375063E-5	0.0	0.0	5.612099671905938	0.0
23	5.203712328375063E-5	0.0	0.0	5.816657603534361	0.0
24	5.203712328375063E-5	0.0	0.0	5.985413994343565	0.0
25	5.203712328375063E-5	0.0	0.0	6.139808139126453	0.0
26	5.203712328375063E-5	0.0	0.0	6.278903369663918	0.0
27	5.203712328375063E-5	0.0	0.0	6.427157133899324	0.0
28	5.203712328375063E-5	0.0	0.0	6.585922397038047	0.0
29	5.203712328375063E-5	0.0	0.0	6.7576969409977075	0.0
30	5.203712328375063E-5	0.0	0.0	6.9963391883769885	0.0
31	5.203712328375063E-5	0.0	0.0	7.185598205759989	0.0
32	5.203712328375063E-5	0.0	0.0	7.403841900812039	0.0
33	5.203712328375063E-5	0.0	0.0	7.59060313627742	0.0
34	5.203712328375063E-5	0.0	0.0	7.76971491462009	0.0
35	5.203712328375063E-5	0.0	0.0	7.967820242961329	0.0
36	5.203712328375063E-5	0.0	0.0	8.152760179111779	0.0
37	1.0407424656750126E-4	0.0	0.0	8.33926122896074	0.0
38	1.0407424656750126E-4	0.0	0.0	8.560419002916682	0.0
39	1.0407424656750126E-4	0.0	0.0	8.854428749469871	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10460	0.0	42.031548	1
CGCATCG	70	0.0	41.785713	21
ATAACGC	95	0.0	40.263157	11
CACGACC	1980	0.0	39.886364	27
GCGAGAC	1985	0.0	39.559196	21
ACGACCA	2005	0.0	39.16459	28
GACACGA	1995	0.0	39.022556	25
ACACGAC	2005	0.0	38.94015	26
TCAAGCG	2045	0.0	38.72861	17
CGACCAA	2045	0.0	38.50856	29
AAACACG	2005	0.0	38.266834	40
AACACGT	1765	0.0	37.988667	41
AGGGCGA	2375	0.0	37.8	6
CGAGACA	2085	0.0	37.55396	22
AGACACG	2080	0.0	37.427883	24
GACCGAT	1630	0.0	37.1319	9
GTCAAGC	2130	0.0	37.07746	16
TAGGGCG	900	0.0	37.0	5
GGGCGAT	5165	0.0	36.984512	7
AGGGATT	2490	0.0	36.957832	6
>>END_MODULE