FastQCFastQC Report
Thu 26 May 2016
SRR1546981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546981_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1625466
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124810.7678413451896255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA207.03415E-445.01
CGTTTTT85200.042.3591581
GCGATAT2050.039.5121969
CATACGA2800.039.37518
TAATACG356.2498875E-638.5714261
TATCGCG356.2498875E-638.5714261
TAGGGTA9400.038.297875
AGGGCGA20550.038.2116786
GGGCGAT37700.038.1366047
AACACGT9500.037.89473741
GTTCGAC301.14012975E-437.50000433
TTACGGG4250.037.0588263
TAGGGCA10700.037.0093465
TGGGCGA9400.036.622346
GGCGATT10400.036.3461538
ACACGAC11200.036.16071326
TAACGCG250.00210736536.01
CCGTATA250.00210736536.045
TACGAAT1250.036.012
AACGGGA3700.035.878384
Produced by FastQC (version 0.11.5)