##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546981_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1625466
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62118924665296	31.0	31.0	33.0	30.0	34.0
2	32.00541998417685	31.0	31.0	34.0	30.0	34.0
3	32.061870257513846	33.0	31.0	34.0	30.0	34.0
4	35.77483564713135	37.0	35.0	37.0	35.0	37.0
5	35.74352585658513	37.0	35.0	37.0	33.0	37.0
6	35.79494188128204	37.0	35.0	37.0	35.0	37.0
7	36.125682727291746	37.0	35.0	37.0	35.0	37.0
8	36.06407577888433	37.0	35.0	37.0	35.0	37.0
9	37.83255263413692	39.0	38.0	39.0	35.0	39.0
10	37.31957912377128	39.0	37.0	39.0	34.0	39.0
11	37.10731384107696	39.0	37.0	39.0	33.0	39.0
12	36.774677538625845	39.0	35.0	39.0	33.0	39.0
13	36.70750357128356	39.0	35.0	39.0	33.0	39.0
14	37.83201002051104	40.0	36.0	41.0	33.0	41.0
15	37.897435566169946	40.0	36.0	41.0	33.0	41.0
16	37.95250100586539	40.0	36.0	41.0	33.0	41.0
17	37.89030714884224	40.0	36.0	41.0	33.0	41.0
18	37.77602484456765	39.0	36.0	41.0	33.0	41.0
19	37.682499664711536	39.0	36.0	41.0	33.0	41.0
20	37.525467773549245	39.0	35.0	41.0	33.0	41.0
21	37.46915223080643	39.0	35.0	41.0	33.0	41.0
22	37.37664952696642	39.0	35.0	41.0	32.0	41.0
23	37.27253415328281	39.0	35.0	41.0	32.0	41.0
24	37.156630160212515	39.0	35.0	41.0	32.0	41.0
25	37.04155054612031	39.0	35.0	41.0	32.0	41.0
26	36.88267549121298	39.0	35.0	41.0	31.0	41.0
27	36.79090180908121	39.0	35.0	41.0	31.0	41.0
28	36.8103682267116	39.0	35.0	41.0	31.0	41.0
29	36.79342908433643	39.0	35.0	41.0	31.0	41.0
30	36.68850532708774	39.0	35.0	41.0	31.0	41.0
31	36.55122346453263	39.0	35.0	41.0	31.0	41.0
32	36.417985980635706	39.0	35.0	41.0	30.0	41.0
33	36.21702760931327	39.0	35.0	41.0	30.0	41.0
34	35.96303337012278	39.0	35.0	41.0	29.0	41.0
35	35.80969149769974	38.0	35.0	41.0	28.0	41.0
36	35.67690988307353	38.0	35.0	41.0	27.0	41.0
37	35.60271392942086	38.0	35.0	41.0	27.0	41.0
38	35.49019296620169	38.0	35.0	41.0	27.0	41.0
39	35.37317360067821	38.0	35.0	41.0	26.0	41.0
40	35.249198691329134	38.0	35.0	41.0	26.0	41.0
41	35.17230812579285	38.0	34.0	40.0	25.0	41.0
42	35.067584926414945	38.0	34.0	40.0	25.0	41.0
43	34.944725389519064	38.0	34.0	40.0	24.0	41.0
44	34.79209039130932	37.0	34.0	40.0	24.0	41.0
45	34.66767499289435	37.0	34.0	40.0	23.0	41.0
46	34.605002503897346	37.0	34.0	40.0	23.0	41.0
47	34.52983821254951	37.0	34.0	40.0	23.0	41.0
48	34.417264341425785	37.0	34.0	40.0	23.0	41.0
49	34.31965479437896	36.0	34.0	40.0	23.0	41.0
50	34.20056033162182	36.0	34.0	40.0	23.0	41.0
51	33.811546965608635	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	5.0
14	17.0
15	49.0
16	137.0
17	281.0
18	668.0
19	1315.0
20	2276.0
21	3594.0
22	5649.0
23	8529.0
24	12689.0
25	19453.0
26	26170.0
27	29273.0
28	30039.0
29	31707.0
30	35907.0
31	41922.0
32	50866.0
33	66091.0
34	108743.0
35	161503.0
36	125317.0
37	163135.0
38	242951.0
39	456864.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.76566104735503	23.570225399977605	26.104206424496113	15.559907128171243
2	34.835302614757865	24.839399901320604	27.157442850234947	13.167854633686584
3	29.54020570101128	25.277182051177938	31.431847851631474	13.75076439617931
4	25.163614618823154	28.2682012419823	31.324678584479777	15.24350555471477
5	23.174031323940333	31.97655318536346	30.018653112399768	14.83076237829644
6	21.831031839484798	40.62176631193763	27.326317499104874	10.220884349472705
7	86.55813163732738	3.410345094883559	8.40036026591759	1.6311630018714633
8	86.37043161776377	2.979514797602657	8.284947208984992	2.3651063756485833
9	81.11181654983864	4.672506222830868	10.742457855162765	3.473219372167735
10	41.71819035279729	24.952721250398348	19.143248766815177	14.185839629989186
11	32.990908453329695	26.87906114308143	23.39698277294019	16.733047630648688
12	27.763915086504426	22.900509761508392	29.630333701227833	19.70524145075935
13	23.75177333761518	27.075435598160773	30.943803192438352	18.22898787178569
14	21.8531178135993	28.33716608037326	30.796460830309584	19.013255275717857
15	20.799696825402684	23.982845534757416	36.005920763645626	19.211536876194273
16	23.273325926226697	22.594505206506934	34.51933168703621	19.612837180230162
17	23.2185723970849	23.28255404911576	29.844795277169744	23.654078276629594
18	24.727616572724376	22.639723008663363	31.3425196220653	21.29014079654696
19	26.298304609262818	25.7380960290772	27.236004936430536	20.72759442522944
20	27.95229183507991	24.341634952684338	27.28743634133227	20.418636870903484
21	26.41162595834056	25.803677222408833	28.085853533694337	19.698843285556265
22	25.634248886165565	23.138410769588535	27.726448907574813	23.500891436671083
23	23.776566227777142	26.663184588296524	27.238219685923916	22.32202949800242
24	23.146654559369438	24.294263921853794	31.553536032128633	21.005545486648135
25	23.302179190459842	25.11452100505332	29.724152950600015	21.859146853886823
26	22.403236979426207	26.929754298152037	28.298592526696957	22.368416195724794
27	22.642552966349342	25.54621259380387	28.805893202318593	23.005341237528192
28	22.05890495402549	26.471116590565412	30.359416930283377	21.110561525125718
29	22.784419975563928	24.413921915315363	30.122746338588442	22.678911770532263
30	23.803020180059136	24.91716221686581	30.061656165062818	21.21816143801224
31	25.505178207357154	25.112306255559947	27.59510195845376	21.787413578629145
32	26.484528129164193	24.523244411141174	26.99908826145856	21.993139198236076
33	25.917675300498445	24.77646410321717	27.069591120331033	22.236269475953357
34	23.20614519159429	25.975997037157345	28.789590185214582	22.028267586033788
35	23.54204886475632	25.975627912241784	28.818074324532166	21.664248898469733
36	26.161851432143152	26.498370313497794	26.285200674760347	21.054577579598714
37	23.916464570775396	27.439823410640397	27.129758481567745	21.513953537016462
38	24.494698751004325	27.33025483153754	26.921325945913356	21.253720471544778
39	23.962912789317034	27.02012838164563	26.342168953395518	22.674789875641814
40	25.70081441260537	25.296376546787197	27.109518132031063	21.893290908576372
41	22.3110172713548	25.287394507175172	27.921346862991903	24.48024135847812
42	24.45913971747179	26.05302110287142	26.335217100819087	23.1526220788377
43	23.74174544407573	25.476324943124002	27.540471470950482	23.241458141849783
44	23.268957948059203	26.485327899814575	27.21564154525533	23.030072606870892
45	22.306280168271744	26.988875805461326	27.04578256327724	23.65906146298969
46	24.250030452805536	26.280525092496553	27.055933498455214	22.413510956242703
47	22.935515107667587	26.36185561555886	27.953706813922903	22.748922462850654
48	23.63660636395963	25.514652413523258	28.437321974129265	22.411419248387848
49	23.59255745736915	24.868253165553757	28.068935308397712	23.470254068679385
50	22.394501023091223	26.43297368262394	28.595922646182693	22.576602648102146
51	22.35801917726978	26.67776502246125	27.15830414170459	23.805911658564373
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	969.0
1	1422.0
2	1875.0
3	11185.5
4	20496.0
5	14284.5
6	8073.0
7	8142.0
8	8211.0
9	8673.5
10	9136.0
11	9280.0
12	9424.0
13	9455.0
14	9486.0
15	9443.5
16	9401.0
17	9162.0
18	8923.0
19	8692.0
20	8461.0
21	8601.0
22	8741.0
23	9111.5
24	9482.0
25	10483.0
26	12367.0
27	13250.0
28	15362.5
29	17475.0
30	19751.0
31	22027.0
32	24437.5
33	26848.0
34	30136.5
35	33425.0
36	38850.5
37	44276.0
38	48758.5
39	53241.0
40	61927.5
41	70614.0
42	79934.5
43	89255.0
44	95445.5
45	101636.0
46	108315.5
47	114995.0
48	121128.0
49	127261.0
50	126847.0
51	126433.0
52	117210.0
53	107987.0
54	103700.5
55	99414.0
56	92531.5
57	85649.0
58	81929.0
59	78209.0
60	73708.0
61	69207.0
62	62981.5
63	56756.0
64	52203.0
65	47650.0
66	41186.0
67	34722.0
68	29805.0
69	24888.0
70	21549.0
71	18210.0
72	16134.0
73	14058.0
74	11996.5
75	8425.5
76	6916.0
77	5256.0
78	3596.0
79	2740.5
80	1885.0
81	1317.5
82	750.0
83	602.5
84	455.0
85	306.0
86	157.0
87	108.5
88	60.0
89	52.5
90	45.0
91	27.5
92	10.0
93	8.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1625466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.742395210982124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05140089785114	20.821056248460447
2	6.8308499416562904	3.7900827741668595
3	2.9013667758382904	2.4147259124195646
4	1.7873326007747308	1.9833954953666046
5	1.3352347566509302	1.8521305159224823
6	1.046144238490868	1.7413528147143353
7	0.8317427968975811	1.6152176169794232
8	0.741275390486686	1.6451803874445392
9	0.6475599587297736	1.6168377868099788
>10	8.392374203695294	53.168931507393246
>50	0.40313122759503645	6.7564813322154835
>100	0.029346984515355477	1.423902039939933
>500	0.0020162041361709887	0.39502068223549963
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.2402268179677652E-4	0.7756848859316033
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12481	0.7678413451896255	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.152081926044592E-5	0.0	0.0	0.05875238239372586	0.0
2	6.152081926044592E-5	0.0	0.0	0.15355596487407303	0.0
3	6.152081926044592E-5	0.0	0.0	0.2622017316880205	0.0
4	6.152081926044592E-5	0.0	0.0	0.41680355048952117	0.0
5	6.152081926044592E-5	0.0	0.0	0.7011527771113022	0.0
6	6.152081926044592E-5	0.0	0.0	1.3557958148617073	0.0
7	6.152081926044592E-5	0.0	0.0	1.6704132845596278	0.0
8	6.152081926044592E-5	0.0	0.0	2.441515233170057	0.0
9	6.152081926044592E-5	0.0	0.0	2.8965232124203153	0.0
10	6.152081926044592E-5	0.0	0.0	3.5373855866563804	0.0
11	6.152081926044592E-5	0.0	0.0	4.025553287488019	0.0
12	6.152081926044592E-5	0.0	0.0	4.449001086457669	0.0
13	6.152081926044592E-5	0.0	0.0	4.717539462529515	0.0
14	6.152081926044592E-5	0.0	0.0	4.867342657428701	0.0
15	6.152081926044592E-5	0.0	0.0	4.992476003804447	0.0
16	1.2304163852089184E-4	0.0	0.0	5.189465667076395	0.0
17	1.2304163852089184E-4	0.0	0.0	5.3922382873588255	0.0
18	1.2304163852089184E-4	0.0	0.0	5.666682662079674	0.0
19	1.2304163852089184E-4	0.0	0.0	5.8305126037702415	0.0
20	1.2304163852089184E-4	0.0	0.0	6.012060541407818	0.0
21	1.2304163852089184E-4	0.0	0.0	6.223630638844491	0.0
22	1.2304163852089184E-4	0.0	0.0	6.4510115868310995	0.0
23	1.2304163852089184E-4	0.0	0.0	6.703739112353011	0.0
24	1.2304163852089184E-4	0.0	0.0	6.910756669164412	0.0
25	1.2304163852089184E-4	0.0	0.0	7.098210605450991	0.0
26	1.2304163852089184E-4	0.0	0.0	7.269423045452812	0.0
27	1.2304163852089184E-4	0.0	0.0	7.440450922996852	0.0
28	1.2304163852089184E-4	0.0	0.0	7.6215066940803435	0.0
29	1.2304163852089184E-4	0.0	0.0	7.820649586026407	0.0
30	1.2304163852089184E-4	0.0	0.0	8.11078176965867	0.0
31	1.2304163852089184E-4	0.0	0.0	8.332502802273318	0.0
32	1.8456245778133778E-4	0.0	0.0	8.587629639746387	0.0
33	1.8456245778133778E-4	0.0	0.0	8.805167256651323	0.0
34	1.8456245778133778E-4	0.0	0.0	9.020613165701405	0.0
35	1.8456245778133778E-4	0.0	0.0	9.247809551230231	0.0
36	1.8456245778133778E-4	0.0	0.0	9.462455689629927	0.0
37	1.8456245778133778E-4	0.0	0.0	9.687621888123159	0.0
38	2.460832770417837E-4	0.0	0.0	9.925461375384044	0.0
39	2.460832770417837E-4	0.0	0.0	10.234665012987046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTA	20	7.03415E-4	45.0	1
CGTTTTT	8520	0.0	42.359158	1
GCGATAT	205	0.0	39.512196	9
CATACGA	280	0.0	39.375	18
TAATACG	35	6.2498875E-6	38.571426	1
TATCGCG	35	6.2498875E-6	38.571426	1
TAGGGTA	940	0.0	38.29787	5
AGGGCGA	2055	0.0	38.211678	6
GGGCGAT	3770	0.0	38.136604	7
AACACGT	950	0.0	37.894737	41
GTTCGAC	30	1.14012975E-4	37.500004	33
TTACGGG	425	0.0	37.058826	3
TAGGGCA	1070	0.0	37.009346	5
TGGGCGA	940	0.0	36.62234	6
GGCGATT	1040	0.0	36.346153	8
ACACGAC	1120	0.0	36.160713	26
TAACGCG	25	0.002107365	36.0	1
CCGTATA	25	0.002107365	36.0	45
TACGAAT	125	0.0	36.0	12
AACGGGA	370	0.0	35.87838	4
>>END_MODULE