FastQCFastQC Report
Thu 26 May 2016
SRR1546980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546980_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2825366
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155070.5488492464339133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG3800.043.2236862
CGTTTTT98050.040.7776641
TACGGGA7100.039.2957764
CGGTCTA1150.039.13043231
ATACCGG356.2515264E-638.5714261
TACGTAC356.2515264E-638.57142639
CACGACC21900.038.21917727
GTACGGG10400.037.8605773
TCACGAC1200.037.50000425
ATATGCG1500.037.5000041
GTACGTC301.1403433E-437.50000435
TACGTCA301.1403433E-437.50000420
AGACACG22400.037.4665224
AACACGT21050.037.41092741
ACACGTG21250.037.16470342
GCGTTAG2000.037.1249961
TCAAGCG22800.037.10526317
CGAGACA22850.036.92560222
GGGCGAT70100.036.9115567
GCGATAT3050.036.8852469
Produced by FastQC (version 0.11.5)