FastQCFastQC Report
Thu 26 May 2016
SRR1546979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546979_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2307515
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148050.6415992962125924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104150.041.694671
TATTGCG850.039.7058831
CGTTAGG4000.039.3752
GTATGCG750.039.01
TCGTTAG1100.038.863641
CGGTCTA700.038.57142631
CGAATAT3000.038.2514
GGGCGAT60800.038.079777
TACGAAT3150.037.85714312
AGGGCGA31150.037.7768866
CGTAAGG3650.036.9862982
TGGTCAA26600.036.71052614
CACGACC26300.036.6159727
TAGGGCG12800.036.56255
ACACGAC26300.036.44486626
GCGATCG1050.036.428579
ATAGGGC10450.036.3875584
AACACGT24750.036.27272841
ACGACCA26600.036.20300728
GGCGATT16600.036.054228
Produced by FastQC (version 0.11.5)