FastQCFastQC Report
Thu 26 May 2016
SRR1546978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546978_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2084182
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94760.4546627885664496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG900.045.01
CCGTACT207.03461E-445.020
ACGGGTA2100.041.7857135
TACGGGA5750.041.0869564
TATAGCG1100.040.9090921
CGTTTTT64050.040.081971
CGGGTAT2350.039.2553186
CGGTCTA1500.039.00000431
TAACGGG5650.038.628323
TTCGACT356.250737E-638.57142631
TTAACGG2750.038.4545442
CTTAACG659.094947E-1238.076921
TGGGCGA12850.037.821016
GTTGATC11650.037.66094616
AAGCGAT301.1402405E-437.50000443
TATTACG301.1402405E-437.5000041
TAGCATA10900.037.36238530
TACGGGT2350.037.3404274
AACACGT18700.037.29946541
ACGATTG1150.037.1739121
Produced by FastQC (version 0.11.5)