##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546978_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2084182
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617381303552186	31.0	31.0	33.0	30.0	34.0
2	31.99927885376613	31.0	31.0	34.0	30.0	34.0
3	32.10326689319839	33.0	31.0	34.0	30.0	34.0
4	35.777652335544595	37.0	35.0	37.0	35.0	37.0
5	35.72798728709873	37.0	35.0	37.0	33.0	37.0
6	35.776743585732916	37.0	35.0	37.0	33.0	37.0
7	36.10790900218887	37.0	35.0	37.0	35.0	37.0
8	36.036394614289925	37.0	35.0	37.0	35.0	37.0
9	37.807188623642276	39.0	38.0	39.0	35.0	39.0
10	37.33429806034214	39.0	37.0	39.0	34.0	39.0
11	37.08941013788623	39.0	37.0	39.0	33.0	39.0
12	36.626677036842274	39.0	35.0	39.0	33.0	39.0
13	36.4805074604809	39.0	35.0	39.0	32.0	39.0
14	37.561137654964874	40.0	35.0	41.0	33.0	41.0
15	37.658790355160924	40.0	35.0	41.0	33.0	41.0
16	37.73168226191379	40.0	35.0	41.0	33.0	41.0
17	37.65021960654108	40.0	35.0	41.0	33.0	41.0
18	37.58044163129708	39.0	36.0	41.0	33.0	41.0
19	37.52445851657868	39.0	36.0	41.0	33.0	41.0
20	37.40827000712989	39.0	35.0	41.0	32.0	41.0
21	37.33353277208996	39.0	35.0	41.0	32.0	41.0
22	37.252499061982114	39.0	35.0	41.0	32.0	41.0
23	37.16796181907338	39.0	35.0	41.0	32.0	41.0
24	37.065707313468785	39.0	35.0	41.0	32.0	41.0
25	36.95928186693868	39.0	35.0	41.0	32.0	41.0
26	36.824995609788395	39.0	35.0	41.0	31.0	41.0
27	36.707547133599654	38.0	35.0	41.0	31.0	41.0
28	36.74044157372053	38.0	35.0	41.0	31.0	41.0
29	36.72704303175058	38.0	35.0	41.0	31.0	41.0
30	36.64158504391651	38.0	35.0	41.0	31.0	41.0
31	36.51676341125679	38.0	35.0	41.0	31.0	41.0
32	36.42001898106787	38.0	35.0	41.0	30.0	41.0
33	36.27919442735807	38.0	35.0	41.0	30.0	41.0
34	36.11441563164829	38.0	35.0	41.0	30.0	41.0
35	35.99963198991259	38.0	35.0	41.0	30.0	41.0
36	35.882697384393495	38.0	35.0	41.0	29.0	41.0
37	35.80723996272878	38.0	35.0	41.0	29.0	41.0
38	35.68093573401939	38.0	35.0	41.0	29.0	41.0
39	35.590183582815705	38.0	35.0	40.0	28.0	41.0
40	35.43708131055733	38.0	35.0	40.0	27.0	41.0
41	35.36530542918037	38.0	35.0	40.0	27.0	41.0
42	35.26594222577491	38.0	34.0	40.0	27.0	41.0
43	35.12374735027939	37.0	34.0	40.0	27.0	41.0
44	34.9689571256253	37.0	34.0	40.0	26.0	41.0
45	34.84664487074545	37.0	34.0	40.0	26.0	41.0
46	34.78065831103042	37.0	34.0	40.0	26.0	41.0
47	34.700054985601064	36.0	34.0	40.0	26.0	41.0
48	34.60532189607242	36.0	34.0	40.0	25.0	41.0
49	34.518602022280206	36.0	34.0	40.0	25.0	41.0
50	34.403765122239804	36.0	34.0	40.0	24.0	41.0
51	34.034912977849345	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	4.0
13	7.0
14	27.0
15	65.0
16	168.0
17	374.0
18	931.0
19	1657.0
20	2779.0
21	4549.0
22	7002.0
23	10291.0
24	15006.0
25	21413.0
26	27397.0
27	32541.0
28	35679.0
29	40381.0
30	47065.0
31	56327.0
32	68054.0
33	88021.0
34	150639.0
35	243489.0
36	153914.0
37	202583.0
38	299439.0
39	573909.0
40	462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.723463689831306	24.03173043429029	26.82164993268342	13.423155943194981
2	31.366166678341912	26.179671449038523	28.238752661715722	14.215409210903845
3	30.492778461765813	25.865111588143453	29.46969122658194	14.172418723508793
4	25.795348007035855	29.730944802325325	29.290772111072833	15.182935079565988
5	23.650093897749812	32.896359339059636	28.29464989142023	15.158896871770317
6	22.126090715686058	41.749424954250635	25.624681529732047	10.499802800331256
7	88.13999928988927	3.7781249430232102	6.29853822746766	1.7833375396198603
8	88.07335443833601	3.0731001419261847	6.179402758492301	2.674142661245515
9	82.70784413261414	4.962474486393223	8.766125031307247	3.563556349685392
10	44.06347430310789	27.527874245147498	14.639748352111285	13.768903099633334
11	35.29461438588377	25.90157673370176	22.40687233648501	16.396936543929467
12	31.13797163587441	22.23759729236698	26.391409195550104	20.233021876208507
13	24.060278804826066	29.946712907030193	27.89463684073656	18.098371447407185
14	21.03683843349573	31.201545738328036	28.933269743237393	18.82834608493884
15	20.016342142864683	24.009851346955305	36.80825378973621	19.1655527204438
16	23.11218502031013	21.964780427045238	35.44143457721063	19.48159997543401
17	22.178581333108145	22.487479500350737	28.689864896635708	26.64407426990541
18	24.57438937674349	22.7133714809935	30.542630154180394	22.169608988082615
19	26.96621504264023	25.811037615716863	26.14109516347421	21.0816521781687
20	29.234251135457463	25.091474736851193	25.15073059838344	20.5235435293079
21	26.336951379486052	26.650839514015573	27.225165556558878	19.787043549939494
22	25.984774842120313	23.37530983378611	26.005406437633567	24.63450888646001
23	23.208481792856862	27.758900134441234	25.467257657920467	23.565360414781434
24	22.381826539141013	25.1995747012497	31.58433380578088	20.83426495382841
25	23.397284882030455	25.157255940220193	28.96076254377017	22.48469663397918
26	21.952545411101333	28.402222070817235	26.20438138319974	23.440851134881694
27	22.320555498512125	26.455079258913088	27.667737270545473	23.556627972029315
28	20.99855003065951	27.49500763369034	30.616328132571912	20.890114203078234
29	21.655114572527733	25.304124112001738	29.457264288819307	23.583497026651223
30	23.953474312703975	26.215320926867232	28.835965381142337	20.995239379286453
31	26.43238450384851	25.943799533821903	25.37978928903522	22.244026673294368
32	26.522203915013183	25.77879475017057	25.49182365071764	22.207177684098607
33	25.2823889660308	25.542538991316498	25.812572990266684	23.36249905238602
34	22.059445864132787	27.29392154811816	27.735917496648565	22.91071509110049
35	21.704294538576764	26.963864000360815	28.520397930698948	22.81144353036347
36	26.012651486290544	27.180159890067184	24.77696285641081	22.03022576723146
37	23.187034529614017	28.200032434787364	26.351201574526602	22.261731461072017
38	23.390711559739024	29.00097016479367	24.976465586978488	22.63185268848882
39	23.08397251295712	27.041352434672213	25.3750392240217	24.499635828348964
40	24.957513307379106	25.123237797850667	25.98319148711581	23.936057407654417
41	20.695457498433438	24.740881554489963	27.12920464719492	27.43445629988168
42	23.870851969741604	25.44518664876676	24.959624447385114	25.724336934106525
43	23.08411645432117	24.65048637786911	27.203190508314535	25.06220665949519
44	22.347568494498084	26.662834627686067	26.989629504525038	23.99996737329082
45	21.85461730309541	27.606562190825944	25.528000913547856	25.010819592530787
46	24.28684251183438	26.10990786793092	26.504595088144896	23.098654532089807
47	22.903613983807556	25.215984016750937	28.099033577681798	23.781368421759712
48	23.879296529765632	24.20671515251547	28.513296823406016	23.400691494312877
49	23.544392956085407	23.797010049986035	28.135306801421372	24.52329019250718
50	22.423377612895614	26.0833266960371	28.10743015725114	23.385865533816144
51	22.466032237107893	26.441500790238088	26.549456813272542	24.543010159381474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1340.0
1	2111.5
2	2883.0
3	9949.0
4	17015.0
5	12047.0
6	7079.0
7	7520.0
8	7961.0
9	8593.5
10	9226.0
11	9454.5
12	9683.0
13	9703.5
14	9724.0
15	9929.0
16	10134.0
17	9785.5
18	9437.0
19	9496.0
20	9555.0
21	9558.5
22	9562.0
23	9717.5
24	9873.0
25	11387.5
26	13778.5
27	14655.0
28	17358.0
29	20061.0
30	22719.5
31	25378.0
32	29381.5
33	33385.0
34	39469.0
35	45553.0
36	49868.0
37	54183.0
38	58698.5
39	63214.0
40	78148.0
41	93082.0
42	108713.5
43	124345.0
44	129508.0
45	134671.0
46	145334.5
47	155998.0
48	162243.0
49	168488.0
50	172170.5
51	175853.0
52	160831.0
53	145809.0
54	134892.5
55	123976.0
56	115319.5
57	106663.0
58	101388.0
59	96113.0
60	92790.0
61	89467.0
62	83555.5
63	77644.0
64	70235.5
65	62827.0
66	53348.5
67	43870.0
68	38140.0
69	32410.0
70	27434.5
71	22459.0
72	20452.0
73	18445.0
74	15194.5
75	9841.0
76	7738.0
77	6272.5
78	4807.0
79	3707.0
80	2607.0
81	1936.0
82	1265.0
83	920.5
84	576.0
85	362.5
86	149.0
87	126.5
88	104.0
89	64.5
90	25.0
91	28.5
92	32.0
93	18.5
94	5.0
95	4.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2084182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.014140055349767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47199694285098	17.274935311229004
2	7.374887242220391	3.247036012853038
3	2.4759733155331944	1.635192700243694
4	1.3142239150710973	1.1572603732186093
5	0.8622259014800628	0.9490580877266156
6	0.643062282688294	0.8493877893247817
7	0.49723353042801005	0.7662318005340762
8	0.403349672693946	0.7103516948771212
9	0.35568016197857777	0.7046993610635313
>10	5.056867115405122	28.066982033651744
>50	2.2525480698095155	35.04453718249877
>100	0.2873246897951954	8.251482943953597
>500	0.0026440914546725664	0.3921238486542646
>1k	0.0017627276364483775	0.4910764279782007
>5k	2.2034095455604718E-4	0.45964443219295037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9476	0.4546627885664496	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.59609093639615E-5	0.0	0.0	0.09044315707553371	0.0
2	9.59609093639615E-5	0.0	0.0	0.2670112303052229	0.0
3	9.59609093639615E-5	0.0	0.0	0.39473520066865564	0.0
4	9.59609093639615E-5	0.0	0.0	0.6567564636869525	0.0
5	9.59609093639615E-5	0.0	0.0	1.0456380488844064	0.0
6	9.59609093639615E-5	0.0	0.0	1.767168126392033	0.0
7	9.59609093639615E-5	0.0	0.0	2.097945380969608	0.0
8	9.59609093639615E-5	0.0	0.0	3.018786267226183	0.0
9	9.59609093639615E-5	0.0	0.0	3.4459082748051753	0.0
10	9.59609093639615E-5	0.0	0.0	4.114995715345397	0.0
11	9.59609093639615E-5	0.0	0.0	4.7287616916372945	0.0
12	9.59609093639615E-5	0.0	0.0	5.234043859893234	0.0
13	9.59609093639615E-5	0.0	0.0	5.506716783850931	0.0
14	9.59609093639615E-5	0.0	0.0	5.639142838773197	0.0
15	9.59609093639615E-5	0.0	0.0	5.779821531900765	0.0
16	9.59609093639615E-5	0.0	0.0	6.028888072154927	0.0
17	9.59609093639615E-5	0.0	0.0	6.298250344739567	0.0
18	9.59609093639615E-5	0.0	0.0	6.644669227543468	0.0
19	9.59609093639615E-5	0.0	0.0	6.823444401688528	0.0
20	9.59609093639615E-5	0.0	0.0	7.014070748140037	0.0
21	9.59609093639615E-5	0.0	0.0	7.237851588776796	0.0
22	9.59609093639615E-5	0.0	0.0	7.462927901689968	0.0
23	9.59609093639615E-5	0.0	0.0	7.720870826060296	0.0
24	9.59609093639615E-5	0.0	0.0	7.929393882108184	0.0
25	9.59609093639615E-5	0.0	0.0	8.10466648306146	0.0
26	9.59609093639615E-5	0.0	0.0	8.275860745366767	0.0
27	9.59609093639615E-5	0.0	0.0	8.457898590430203	0.0
28	9.59609093639615E-5	0.0	0.0	8.640704122768549	0.0
29	9.59609093639615E-5	0.0	0.0	8.82965115330619	0.0
30	9.59609093639615E-5	0.0	0.0	9.06705844307263	0.0
31	9.59609093639615E-5	0.0	0.0	9.263634365904705	0.0
32	9.59609093639615E-5	0.0	0.0	9.477723154695703	0.0
33	9.59609093639615E-5	0.0	0.0	9.69272357212566	0.0
34	9.59609093639615E-5	0.0	0.0	9.89740819179899	0.0
35	9.59609093639615E-5	0.0	0.0	10.119221833793786	0.0
36	9.59609093639615E-5	0.0	0.0	10.318868505725508	0.0
37	9.59609093639615E-5	0.0	0.0	10.524512734492477	0.0
38	9.59609093639615E-5	0.0	0.0	10.747765790127733	0.0
39	9.59609093639615E-5	0.0	0.0	11.009883014055394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	90	0.0	45.0	1
CCGTACT	20	7.03461E-4	45.0	20
ACGGGTA	210	0.0	41.785713	5
TACGGGA	575	0.0	41.086956	4
TATAGCG	110	0.0	40.909092	1
CGTTTTT	6405	0.0	40.08197	1
CGGGTAT	235	0.0	39.255318	6
CGGTCTA	150	0.0	39.000004	31
TAACGGG	565	0.0	38.62832	3
TTCGACT	35	6.250737E-6	38.571426	31
TTAACGG	275	0.0	38.454544	2
CTTAACG	65	9.094947E-12	38.07692	1
TGGGCGA	1285	0.0	37.82101	6
GTTGATC	1165	0.0	37.660946	16
AAGCGAT	30	1.1402405E-4	37.500004	43
TATTACG	30	1.1402405E-4	37.500004	1
TAGCATA	1090	0.0	37.362385	30
TACGGGT	235	0.0	37.340427	4
AACACGT	1870	0.0	37.299465	41
ACGATTG	115	0.0	37.173912	1
>>END_MODULE