FastQCFastQC Report
Thu 26 May 2016
SRR1546977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546977_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1923113
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115350.5998087475878953No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCT253.8917886E-545.019
CGTTTTT86200.042.7813221
TCGATTG603.6379788E-1241.2500041
AGGGCGA21400.041.1098146
GCGATAT3150.040.09
TAGGGTA10850.039.400925
GGCGATA9200.039.1304328
AGGGTAC12100.038.8636366
GGGCGAT44050.038.7684447
CACGACC10200.037.527
GACCGAT11700.037.307699
TAAGGGA15750.037.04
CGTTAGG2800.036.9642832
ACACGAC10350.036.95652426
GTAGGGC11100.036.486494
TATGGGC9600.036.328134
GTATCGC250.002107460336.010
GGTTACG504.8818038E-836.01
TCGAACG250.002107460336.01
TCAAGCG10650.035.91549317
Produced by FastQC (version 0.11.5)