##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546977_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1923113
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.602066025241367	31.0	31.0	33.0	30.0	34.0
2	31.982302132012002	31.0	31.0	34.0	30.0	34.0
3	32.04993206327449	33.0	31.0	34.0	30.0	34.0
4	35.75078323530651	37.0	35.0	37.0	35.0	37.0
5	35.708281312642576	37.0	35.0	37.0	33.0	37.0
6	35.77075762058704	37.0	35.0	37.0	33.0	37.0
7	36.130352714583076	37.0	35.0	37.0	35.0	37.0
8	36.065387213335875	37.0	35.0	37.0	35.0	37.0
9	37.84774737625922	39.0	38.0	39.0	35.0	39.0
10	37.258773145415795	39.0	37.0	39.0	34.0	39.0
11	37.05060441066126	39.0	37.0	39.0	33.0	39.0
12	36.679584091002454	39.0	35.0	39.0	33.0	39.0
13	36.59741055257803	39.0	35.0	39.0	33.0	39.0
14	37.68241387791565	40.0	36.0	41.0	33.0	41.0
15	37.7711190138073	40.0	36.0	41.0	33.0	41.0
16	37.837071456539476	40.0	36.0	41.0	33.0	41.0
17	37.78207728823007	40.0	36.0	41.0	33.0	41.0
18	37.683976448601825	39.0	36.0	41.0	33.0	41.0
19	37.60970208198894	39.0	36.0	41.0	33.0	41.0
20	37.46967494889796	39.0	35.0	41.0	33.0	41.0
21	37.417672804458185	39.0	35.0	41.0	33.0	41.0
22	37.32837591966775	39.0	35.0	41.0	32.0	41.0
23	37.23815917213393	39.0	35.0	41.0	32.0	41.0
24	37.156303347749194	39.0	35.0	41.0	32.0	41.0
25	37.04780894310423	39.0	35.0	41.0	32.0	41.0
26	36.900705782759516	39.0	35.0	41.0	32.0	41.0
27	36.81341502033422	38.0	35.0	41.0	31.0	41.0
28	36.836329430459884	39.0	35.0	41.0	31.0	41.0
29	36.844987788029094	39.0	35.0	41.0	31.0	41.0
30	36.758802524864635	39.0	35.0	41.0	31.0	41.0
31	36.63647689969336	39.0	35.0	41.0	31.0	41.0
32	36.509557160707665	39.0	35.0	41.0	31.0	41.0
33	36.365738778740514	39.0	35.0	41.0	30.0	41.0
34	36.17144130376114	39.0	35.0	41.0	30.0	41.0
35	36.043340146938846	39.0	35.0	41.0	30.0	41.0
36	35.94440576294789	39.0	35.0	41.0	29.0	41.0
37	35.846607557642216	38.0	35.0	41.0	29.0	41.0
38	35.75049464072054	38.0	35.0	41.0	29.0	41.0
39	35.64940697712511	38.0	35.0	41.0	28.0	41.0
40	35.53192662105659	38.0	35.0	41.0	27.0	41.0
41	35.46518587311302	38.0	35.0	41.0	27.0	41.0
42	35.37593526745438	38.0	35.0	40.0	27.0	41.0
43	35.258414872136996	38.0	35.0	40.0	26.0	41.0
44	35.124799218766654	38.0	34.0	40.0	26.0	41.0
45	35.02519924726212	38.0	34.0	40.0	26.0	41.0
46	34.97426932270751	37.0	34.0	40.0	26.0	41.0
47	34.91501591430145	37.0	34.0	40.0	25.0	41.0
48	34.813198704392306	37.0	34.0	40.0	25.0	41.0
49	34.73325436414813	37.0	34.0	40.0	25.0	41.0
50	34.62217040808314	37.0	34.0	40.0	24.0	41.0
51	34.24227593490346	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	2.0
13	3.0
14	28.0
15	48.0
16	113.0
17	290.0
18	700.0
19	1364.0
20	2371.0
21	3925.0
22	6006.0
23	9251.0
24	14357.0
25	21202.0
26	27876.0
27	30668.0
28	32255.0
29	35200.0
30	41299.0
31	49376.0
32	60952.0
33	79169.0
34	132976.0
35	205668.0
36	142181.0
37	186448.0
38	279680.0
39	559288.0
40	410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.109644103076626	24.449005336659884	27.688440564855004	13.752909995408485
2	33.47873993883875	25.45601844509397	27.440093223851118	13.625148392216163
3	29.68140717680136	24.72506815772136	31.61571888911364	13.977805776363637
4	24.98984719046671	28.821759303795464	30.85726111778143	15.331132387956403
5	22.74005739652324	32.597148477494564	30.304355490291	14.358438635691195
6	21.159807042019892	41.569320159553804	26.73457045945818	10.53630233896812
7	87.394812473318	3.366104851872979	7.555926250823535	1.6831564239854861
8	87.12779748251923	2.729116801768799	7.323750606438622	2.81933510927335
9	81.87641599843587	4.591825857346916	10.195760727528752	3.335997416688463
10	41.37962771818401	23.105922532893285	19.749957490797474	15.764492258125237
11	34.05572111467189	26.495478944814995	22.668142745642093	16.780657194871022
12	28.320852700803336	22.931725800823976	28.35070014086536	20.39672135750733
13	22.92241797543878	28.45189024253905	31.03582576790859	17.589866014113575
14	21.34897949314471	30.00333313747034	30.219753077432266	18.42793429195268
15	20.550222477826317	24.775767206607206	36.83740893020847	17.836601385358012
16	22.19723957978548	22.65935491050188	35.60700801252968	19.536397497182953
17	22.076341847826935	24.38457854530649	29.331401742903303	24.207677863963273
18	24.005297660615888	23.716599076601323	31.567567792428214	20.71053547035458
19	25.75402485449373	27.46552074683079	26.70737496964557	20.07307942902991
20	27.441497197512575	25.680186239706142	26.72604261944046	20.152273943340823
21	25.06867771160613	27.338331132907946	28.312740852981598	19.280250302504324
22	24.30964795100444	24.183082325375576	27.806998340711132	23.70027138290886
23	22.11627709864163	28.160643706324066	26.949846420881148	22.773232774153158
24	22.69357026862176	25.375367958097105	31.61847483741205	20.312586935869085
25	22.445326925666876	25.665054523577137	29.849208028857376	22.04041052189861
26	20.776678229516417	28.936001160618225	28.443414401545823	21.843906208319535
27	22.264474318461787	27.066116239659344	28.648238559044632	22.021170882834237
28	21.228653750455642	27.381542322266032	30.94513946918356	20.444664458094767
29	21.654941753292707	25.265234024209704	30.592430086011586	22.487394136486
30	23.173781259863567	26.425436258815783	29.945094230032243	20.455688251288407
31	25.46142634364179	26.075846817113714	27.223309290717708	21.23941754852679
32	25.35352836780782	26.017503911626616	27.98561498986279	20.643352730702773
33	25.23751854415211	25.619347381043134	27.35970273197675	21.78343134282801
34	21.407686391803292	27.801122450942824	29.28943853013318	21.501752627120716
35	21.681305258713344	27.405305876461757	29.2764387740086	21.636950090816295
36	24.87347337363951	26.956242300894434	26.59458908550876	21.575695239957298
37	22.010251087689596	29.156685020589013	27.508939932286868	21.32412395943452
38	22.835475606477623	28.588439680871584	26.180468854404293	22.395615858246497
39	22.357604571338243	27.42012559844377	26.435680066641947	23.786589763576035
40	23.861312361780094	25.56495640141791	26.880271726102418	23.69345951069958
41	20.414349026812257	25.10289306972601	27.681420696547733	26.801337206914
42	22.906454274917802	26.45403572228985	26.229035943285705	24.410474059506644
43	22.523169465340832	25.24916632563973	28.063613526610244	24.164050682409197
44	22.249758594528767	27.022593056154264	27.275048320093514	23.452600029223454
45	21.50284460663518	27.1316870095517	27.12841107100831	24.23705731280481
46	23.502935084937807	26.788285451764928	26.977405903865243	22.731373559432026
47	22.51094969458373	25.663494552842188	28.46816593720702	23.357389815367064
48	23.03712782348203	24.862969570690854	28.926433340110542	23.173469265716577
49	23.3807373773668	24.357487053542876	28.220234588399123	24.041540980691202
50	21.7194205436706	26.409888550490795	28.551468374453293	23.31922253138531
51	21.182894608897136	26.840752467483707	27.25222074833876	24.724132175280392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1083.0
1	1575.0
2	2067.0
3	10576.5
4	19086.0
5	13415.0
6	7744.0
7	7986.5
8	8229.0
9	8867.5
10	9506.0
11	9798.5
12	10091.0
13	10190.5
14	10290.0
15	10275.0
16	10260.0
17	10341.5
18	10423.0
19	10363.5
20	10304.0
21	10074.0
22	9844.0
23	9709.0
24	9574.0
25	10092.5
26	12326.0
27	14041.0
28	16320.0
29	18599.0
30	21006.5
31	23414.0
32	27364.5
33	31315.0
34	36339.5
35	41364.0
36	46412.5
37	51461.0
38	58637.5
39	65814.0
40	80572.5
41	95331.0
42	110549.0
43	125767.0
44	142125.0
45	158483.0
46	169013.0
47	179543.0
48	173541.0
49	167539.0
50	156778.5
51	146018.0
52	135190.0
53	124362.0
54	115693.0
55	107024.0
56	100331.5
57	93639.0
58	87373.0
59	81107.0
60	75674.0
61	70241.0
62	62803.5
63	55366.0
64	49935.5
65	44505.0
66	38638.5
67	32772.0
68	27401.0
69	22030.0
70	18481.0
71	14932.0
72	13256.5
73	11581.0
74	9780.5
75	6363.5
76	4747.0
77	3582.0
78	2417.0
79	1822.0
80	1227.0
81	975.0
82	723.0
83	553.0
84	383.0
85	288.5
86	194.0
87	117.5
88	41.0
89	31.0
90	21.0
91	15.5
92	10.0
93	8.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1923113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.220975116774376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25593532660659	20.75760918031914
2	7.673998604115496	4.1778745009757845
3	2.89934264373852	2.3676880188062728
4	1.6381777595676934	1.7837118412018156
5	1.1313242258356246	1.5397874300237784
6	0.8815331689809538	1.4397715474465085
7	0.7007647714234071	1.3352850283940054
8	0.6084536879255809	1.325016215898547
9	0.5036188099050221	1.2338095583487716
>10	6.590756364870804	42.04703162031103
>50	1.0507779321685289	18.078438017241524
>100	0.062224802779832965	2.548038354069246
>500	0.0015459510410151815	0.268590246683708
>1k	0.0013527071608882837	0.49057435185512355
>5k	0.0	0.0
>10k+	1.932438801268977E-4	0.6067740884247105
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11535	0.5998087475878953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.199902449830041E-5	0.0	0.0	0.09708217873832686	0.0
2	5.199902449830041E-5	0.0	0.0	0.29395048548889224	0.0
3	5.199902449830041E-5	0.0	0.0	0.45265150825770506	0.0
4	5.199902449830041E-5	0.0	0.0	0.7394261283658319	0.0
5	5.199902449830041E-5	0.0	0.0	1.223069055224524	0.0
6	5.199902449830041E-5	0.0	0.0	2.1931628562648164	0.0
7	5.199902449830041E-5	0.0	0.0	2.6392104884112375	0.0
8	5.199902449830041E-5	0.0	0.0	3.8264522157564325	0.0
9	5.199902449830041E-5	0.0	0.0	4.423245019923426	0.0
10	5.199902449830041E-5	0.0	0.0	5.309620391521455	0.0
11	5.199902449830041E-5	0.0	0.0	6.051022482818222	0.0
12	5.199902449830041E-5	0.0	0.0	6.62841965084735	0.0
13	5.199902449830041E-5	0.0	0.0	6.972809190099594	0.0
14	5.199902449830041E-5	0.0	0.0	7.137854093857199	0.0
15	1.0399804899660082E-4	0.0	0.0	7.305290952741727	0.0
16	1.0399804899660082E-4	0.0	0.0	7.602621374823008	0.0
17	1.0399804899660082E-4	0.0	0.0	7.933439168681195	0.0
18	1.0399804899660082E-4	0.0	0.0	8.352239311990507	0.0
19	1.0399804899660082E-4	0.0	0.0	8.587950890041302	0.0
20	1.0399804899660082E-4	0.0	0.0	8.835258250555219	0.0
21	1.0399804899660082E-4	0.0	0.0	9.141896498021698	0.0
22	1.0399804899660082E-4	0.0	0.0	9.454410635256483	0.0
23	1.0399804899660082E-4	0.0	0.0	9.824227697488395	0.0
24	1.0399804899660082E-4	0.0	0.0	10.116306218095348	0.0
25	1.0399804899660082E-4	0.0	0.0	10.374013383508926	0.0
26	1.0399804899660082E-4	0.0	0.0	10.619344781091907	0.0
27	1.0399804899660082E-4	0.0	0.0	10.867276129899803	0.0
28	1.0399804899660082E-4	0.0	0.0	11.12836323190577	0.0
29	1.0399804899660082E-4	0.0	0.0	11.404634049065239	0.0
30	1.0399804899660082E-4	0.0	0.0	11.755003476134787	0.0
31	1.0399804899660082E-4	0.0	0.0	12.04375405917385	0.0
32	1.0399804899660082E-4	0.0	0.0	12.352524266644759	0.0
33	1.0399804899660082E-4	0.0	0.0	12.630303055514679	0.0
34	1.0399804899660082E-4	0.0	0.0	12.918533648308758	0.0
35	1.0399804899660082E-4	0.0	0.0	13.22106397283987	0.0
36	1.0399804899660082E-4	0.0	0.0	13.512726501250837	0.0
37	1.0399804899660082E-4	0.0	0.0	13.812812871630529	0.0
38	1.0399804899660082E-4	0.0	0.0	14.130422913266147	0.0
39	1.0399804899660082E-4	0.0	0.0	14.52623948774721	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGCT	25	3.8917886E-5	45.0	19
CGTTTTT	8620	0.0	42.781322	1
TCGATTG	60	3.6379788E-12	41.250004	1
AGGGCGA	2140	0.0	41.109814	6
GCGATAT	315	0.0	40.0	9
TAGGGTA	1085	0.0	39.40092	5
GGCGATA	920	0.0	39.130432	8
AGGGTAC	1210	0.0	38.863636	6
GGGCGAT	4405	0.0	38.768444	7
CACGACC	1020	0.0	37.5	27
GACCGAT	1170	0.0	37.30769	9
TAAGGGA	1575	0.0	37.0	4
CGTTAGG	280	0.0	36.964283	2
ACACGAC	1035	0.0	36.956524	26
GTAGGGC	1110	0.0	36.48649	4
TATGGGC	960	0.0	36.32813	4
GTATCGC	25	0.0021074603	36.0	10
GGTTACG	50	4.8818038E-8	36.0	1
TCGAACG	25	0.0021074603	36.0	1
TCAAGCG	1065	0.0	35.915493	17
>>END_MODULE