FastQCFastQC Report
Thu 26 May 2016
SRR1546976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546976_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2523964
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139480.5526227790887667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG351.212702E-745.01
CGATCGA253.8921375E-545.010
TATCGAT207.0348923E-445.040
CGTTAGG4150.041.204822
CGTTTTT87500.040.681
GCGATAA3600.039.3759
TACGGGT1550.039.1935464
AGGGCGA38050.038.909336
GCCAGTA15300.038.08823825
AGGGTAC9950.037.9899526
AACGGGA6400.037.968754
ACGTTAG1900.037.8947371
TAGCATA15500.037.88709630
GGGCGAT70050.037.74097
TAATCGT1850.037.70270521
GTTGATC15650.037.3801916
CCAGTAG15650.037.23642326
CACAACG11950.037.09205212
GGCGATA13050.037.0689668
TACGAAT1400.036.96428312
Produced by FastQC (version 0.11.5)