##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546976_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2523964
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61401192726996	31.0	31.0	33.0	30.0	34.0
2	32.002554315354736	31.0	31.0	34.0	30.0	34.0
3	32.09672324961846	33.0	31.0	34.0	30.0	34.0
4	35.78324611603018	37.0	35.0	37.0	35.0	37.0
5	35.73635915567734	37.0	35.0	37.0	33.0	37.0
6	35.78515026363292	37.0	35.0	37.0	35.0	37.0
7	36.10143369715257	37.0	35.0	37.0	35.0	37.0
8	36.0307001209209	37.0	35.0	37.0	35.0	37.0
9	37.798118356680206	39.0	38.0	39.0	35.0	39.0
10	37.34422559117325	39.0	37.0	39.0	34.0	39.0
11	37.09175844029471	39.0	37.0	39.0	33.0	39.0
12	36.66618620550848	39.0	35.0	39.0	33.0	39.0
13	36.531041647186726	39.0	35.0	39.0	32.0	39.0
14	37.57256918085995	40.0	36.0	41.0	32.0	41.0
15	37.689058956466894	40.0	36.0	41.0	33.0	41.0
16	37.77468775307413	40.0	36.0	41.0	33.0	41.0
17	37.71598208215331	40.0	35.0	41.0	33.0	41.0
18	37.64192714317637	39.0	36.0	41.0	33.0	41.0
19	37.57062224342344	39.0	36.0	41.0	33.0	41.0
20	37.4259133648499	39.0	35.0	41.0	32.0	41.0
21	37.332144198570184	39.0	35.0	41.0	32.0	41.0
22	37.25255114573742	39.0	35.0	41.0	32.0	41.0
23	37.1429243840245	39.0	35.0	41.0	32.0	41.0
24	37.005243339445414	39.0	35.0	41.0	32.0	41.0
25	36.871748963138934	39.0	35.0	41.0	31.0	41.0
26	36.7121975590777	38.0	35.0	41.0	31.0	41.0
27	36.625742680957416	38.0	35.0	41.0	31.0	41.0
28	36.649800868792106	38.0	35.0	41.0	31.0	41.0
29	36.64032093960136	38.0	35.0	41.0	31.0	41.0
30	36.55276739287882	38.0	35.0	41.0	31.0	41.0
31	36.442650925290536	38.0	35.0	41.0	31.0	41.0
32	36.340895908182524	38.0	35.0	41.0	30.0	41.0
33	36.1929294554122	38.0	35.0	41.0	30.0	41.0
34	35.99311083676312	38.0	35.0	41.0	30.0	41.0
35	35.86787886039579	38.0	35.0	41.0	29.0	41.0
36	35.71841674445436	38.0	35.0	41.0	29.0	41.0
37	35.635646546464216	38.0	35.0	41.0	28.0	41.0
38	35.49911250715145	38.0	35.0	40.0	28.0	41.0
39	35.397553213912715	38.0	35.0	40.0	27.0	41.0
40	35.21896706926089	37.0	34.0	40.0	27.0	41.0
41	35.130673020692846	37.0	34.0	40.0	26.0	41.0
42	35.0217122748185	37.0	34.0	40.0	26.0	41.0
43	34.8873256512375	37.0	34.0	40.0	26.0	41.0
44	34.732845634882274	36.0	34.0	40.0	26.0	41.0
45	34.5929537822251	36.0	34.0	40.0	25.0	41.0
46	34.5322159904024	36.0	34.0	40.0	25.0	41.0
47	34.45010626142053	36.0	34.0	40.0	25.0	41.0
48	34.35074509779062	35.0	34.0	40.0	24.0	41.0
49	34.246062542888886	35.0	34.0	40.0	24.0	41.0
50	34.13964105668702	35.0	33.0	40.0	24.0	41.0
51	33.77120949427171	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	7.0
13	8.0
14	39.0
15	73.0
16	208.0
17	487.0
18	1140.0
19	2148.0
20	3511.0
21	5820.0
22	8846.0
23	12982.0
24	18650.0
25	26456.0
26	34606.0
27	40677.0
28	44569.0
29	49929.0
30	57207.0
31	68660.0
32	84107.0
33	110157.0
34	188003.0
35	273714.0
36	207253.0
37	269617.0
38	384660.0
39	629994.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.63664141009935	23.96864614550762	26.873164593472808	13.521547850920218
2	33.376189200796844	25.29077276854979	27.00866573374264	14.324372296910731
3	30.696357000337564	25.6668874833397	29.896345589715224	13.74040992660751
4	26.286428808017863	28.704133656422993	29.387938972188195	15.62149856337095
5	24.64112007936722	31.76083335578479	28.14945062607866	15.448595938769333
6	22.508482688342625	41.487081432223285	25.227420042441175	10.77701583699292
7	87.5777942950058	3.4826170262333376	7.0378579092253295	1.9017307695355403
8	87.23678309199339	3.143507593610685	6.821690008256853	2.7980193061390732
9	81.82846506527034	4.870037765990324	9.536982302441714	3.764514866297618
10	45.925377699523445	25.514111928696288	14.77402213343772	13.786488238342542
11	35.7954788578601	26.335082433822354	21.690919521831532	16.17851918648602
12	30.15688813311125	22.227852695204845	27.113223484962546	20.502035686721364
13	24.87983188349755	26.90902088936292	28.903859167563407	19.307288059576127
14	22.82552366040086	27.59781835240122	29.99131524855347	19.58534273864445
15	21.759383255862602	22.293344913001928	35.59987384923081	20.347397981904656
16	25.086887134681795	20.445339156976882	33.52246703994194	20.94530666839939
17	24.269680550118782	21.715642536898308	28.078332337545227	25.936344575437687
18	26.312380049794687	21.738543022008237	29.760131285549242	22.188945642647834
19	28.38677572263313	24.661366009974785	25.53566532644681	21.416192940945276
20	30.82341903450287	24.18933075115176	23.9815227158549	21.00572749849047
21	27.83763952259224	25.709994278840743	26.003381981676444	20.448984216890572
22	27.958362322125037	22.281102266117898	25.420449737001004	24.34008567475606
23	25.85409300608091	26.79891630783957	24.440721024547102	22.906269661532413
24	24.260369799252288	24.66726942222631	29.64174607878718	21.430614699734228
25	25.41700277816958	23.94146667702075	27.756735040594876	22.8847955042148
26	23.78865150216089	26.28460627806102	25.730081728582498	24.196660491195594
27	24.440522923464837	24.584304688973376	26.273037174856697	24.702135212705095
28	22.538197850682497	26.104373913415564	28.290934419032922	23.06649381686902
29	23.687421849123048	24.30708203445057	27.902180855194448	24.103315261231938
30	24.25577385414372	24.362193755536925	28.631272078365615	22.750760311953737
31	26.806008326584692	24.602846950273456	24.549399278278138	24.041745444863714
32	28.032333266243096	24.289886860509895	25.085500427105934	22.59227944614107
33	26.47331736902745	24.005334465943253	24.4982495788371	25.023098586192194
34	23.575455117426397	26.900145960877413	26.925344418541624	22.599054503154562
35	24.018131795857627	26.402753763524363	26.76409013757724	22.815024303040772
36	27.862005955710938	26.65335163259064	23.536429204220028	21.948213207478396
37	24.29606761427659	26.38670757586083	24.947384352550195	24.369840457312385
38	25.887730569849648	26.7842568277519	24.25002892275801	23.077983679640436
39	24.96347016043018	25.09619788554829	23.947766291436803	25.992565662584727
40	26.87379851693606	23.851092963291077	25.630476504419235	23.644632015353626
41	22.933052927854757	23.41253678737098	25.957264049724955	27.697146235049313
42	25.362406119897113	24.201335676737067	25.028685036712094	25.407573166653723
43	23.988495873950658	23.528782502444567	26.915280883562527	25.56744074004225
44	25.049089448185473	24.697024204782636	26.332229778237725	23.921656568794166
45	23.274262231949425	24.53232296498682	26.021409180162635	26.172005622901118
46	25.01410479705733	24.3212660719408	26.03214625882144	24.63248287218043
47	23.980413349794212	24.574676976375258	27.555385100579883	23.889524573250647
48	24.98545938056169	23.79495111657694	27.013420159717015	24.206169343144353
49	24.678798905214176	23.60505141911691	27.06441137829224	24.651738297376667
50	23.529614526990084	25.346122210934862	26.904979627284696	24.219283634790354
51	23.35853443234531	25.171198955294134	25.73372678849619	25.73653982386437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1583.0
1	2495.5
2	3408.0
3	13359.0
4	23310.0
5	16427.0
6	9544.0
7	9850.0
8	10156.0
9	10619.5
10	11083.0
11	11348.0
12	11613.0
13	11730.5
14	11848.0
15	11658.0
16	11468.0
17	11046.5
18	10625.0
19	10492.5
20	10360.0
21	10271.5
22	10183.0
23	10406.0
24	10629.0
25	11257.5
26	12859.5
27	13833.0
28	16357.5
29	18882.0
30	22809.0
31	26736.0
32	30279.5
33	33823.0
34	38385.0
35	42947.0
36	47556.0
37	52165.0
38	57619.0
39	63073.0
40	76756.5
41	90440.0
42	104111.5
43	117783.0
44	129822.0
45	141861.0
46	155550.5
47	169240.0
48	180463.0
49	191686.0
50	190114.0
51	188542.0
52	180782.5
53	173023.0
54	165435.0
55	157847.0
56	151113.5
57	144380.0
58	138479.0
59	132578.0
60	131435.0
61	130292.0
62	126216.0
63	122140.0
64	112670.0
65	103200.0
66	91214.0
67	79228.0
68	67929.5
69	56631.0
70	49360.5
71	42090.0
72	35518.5
73	28947.0
74	26419.0
75	19286.5
76	14682.0
77	11399.0
78	8116.0
79	6389.0
80	4662.0
81	3344.5
82	2027.0
83	1481.0
84	935.0
85	650.5
86	366.0
87	249.5
88	133.0
89	98.0
90	63.0
91	38.0
92	13.0
93	11.5
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2523964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.537295241079462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20319082331433	16.842852095561966
2	6.8007501538083766	2.929395278467751
3	2.4039165791797026	1.5532158330215708
4	1.3451100195781382	1.1588012649355417
5	0.934552579716583	1.006386741383425
6	0.7139589002974848	0.9226046175422008
7	0.5570832164640994	0.8398645994786242
8	0.4562209771637675	0.7860612704279865
9	0.3759895021984054	0.7288017224754197
>10	5.6691228099196165	31.127383289114626
>50	2.3303013603629443	34.38575433150009
>100	0.20420840427566067	6.019590268540144
>500	0.0031703151085302143	0.44414139895373644
>1k	0.002237869488374269	0.6949298683937013
>5k	0.0	0.0
>10k+	1.8648912403118907E-4	0.5602174202031757
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13948	0.5526227790887667	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1886064935949959E-4	0.0	0.0	0.062441461130190445	0.0
2	1.1886064935949959E-4	0.0	0.0	0.18094552854161153	0.0
3	1.1886064935949959E-4	0.0	0.0	0.28035265162260636	0.0
4	1.1886064935949959E-4	0.0	0.0	0.4950942247987689	0.0
5	1.1886064935949959E-4	0.0	0.0	0.8305585975077299	0.0
6	1.1886064935949959E-4	0.0	0.0	1.582431445139471	0.0
7	1.1886064935949959E-4	0.0	0.0	1.9448771852530384	0.0
8	1.1886064935949959E-4	0.0	0.0	2.9010714891337592	0.0
9	1.1886064935949959E-4	0.0	0.0	3.42346404306876	0.0
10	1.1886064935949959E-4	0.0	0.0	4.1422936301785604	0.0
11	1.1886064935949959E-4	0.0	0.0	4.686794264894428	0.0
12	1.1886064935949959E-4	0.0	0.0	5.131531194581222	0.0
13	1.584808658126661E-4	0.0	0.0	5.373571096893617	0.0
14	1.584808658126661E-4	0.0	0.0	5.497582374392028	0.0
15	1.584808658126661E-4	0.0	0.0	5.6167203652666995	0.0
16	1.9810108226583263E-4	0.0	0.0	5.806104999912836	0.0
17	1.9810108226583263E-4	0.0	0.0	6.001194945728227	0.0
18	1.9810108226583263E-4	0.0	0.0	6.266650395964443	0.0
19	1.9810108226583263E-4	0.0	0.0	6.415345068313178	0.0
20	1.9810108226583263E-4	0.0	0.0	6.57929352399638	0.0
21	1.9810108226583263E-4	0.0	0.0	6.773115622885271	0.0
22	1.9810108226583263E-4	0.0	0.0	6.980210494285973	0.0
23	1.9810108226583263E-4	0.0	0.0	7.2061249685019275	0.0
24	1.9810108226583263E-4	0.0	0.0	7.392775808212796	0.0
25	1.9810108226583263E-4	0.0	0.0	7.562865397446239	0.0
26	1.9810108226583263E-4	0.0	0.0	7.713382599751819	0.0
27	1.9810108226583263E-4	0.0	0.0	7.873527514655518	0.0
28	1.9810108226583263E-4	0.0	0.0	8.023054211549768	0.0
29	1.9810108226583263E-4	0.0	0.0	8.199007592818282	0.0
30	1.9810108226583263E-4	0.0	0.0	8.471911643747692	0.0
31	1.9810108226583263E-4	0.0	0.0	8.668229816273133	0.0
32	1.9810108226583263E-4	0.0	0.0	8.910626300533606	0.0
33	1.9810108226583263E-4	0.0	0.0	9.110470672323377	0.0
34	1.9810108226583263E-4	0.0	0.0	9.321369084503582	0.0
35	1.9810108226583263E-4	0.0	0.0	9.556039626555688	0.0
36	1.9810108226583263E-4	0.0	0.0	9.755051973799944	0.0
37	1.9810108226583263E-4	0.0	0.0	9.954896345589715	0.0
38	1.9810108226583263E-4	0.0	0.0	10.189091445044383	0.0
39	1.9810108226583263E-4	0.0	0.0	10.598764483170125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	35	1.212702E-7	45.0	1
CGATCGA	25	3.8921375E-5	45.0	10
TATCGAT	20	7.0348923E-4	45.0	40
CGTTAGG	415	0.0	41.20482	2
CGTTTTT	8750	0.0	40.68	1
GCGATAA	360	0.0	39.375	9
TACGGGT	155	0.0	39.193546	4
AGGGCGA	3805	0.0	38.90933	6
GCCAGTA	1530	0.0	38.088238	25
AGGGTAC	995	0.0	37.989952	6
AACGGGA	640	0.0	37.96875	4
ACGTTAG	190	0.0	37.894737	1
TAGCATA	1550	0.0	37.887096	30
GGGCGAT	7005	0.0	37.7409	7
TAATCGT	185	0.0	37.702705	21
GTTGATC	1565	0.0	37.38019	16
CCAGTAG	1565	0.0	37.236423	26
CACAACG	1195	0.0	37.092052	12
GGCGATA	1305	0.0	37.068966	8
TACGAAT	140	0.0	36.964283	12
>>END_MODULE