FastQCFastQC Report
Thu 26 May 2016
SRR1546975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546975_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1582859
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311641.9688424553292492No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC18080.11422369269783349No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG16280.10285186488499606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT207.0340926E-445.00000417
CGACGTC207.0340926E-445.00000418
CAACGCG253.8914746E-545.01
ATCCGCG253.8914746E-545.01
TTACGCG253.8914746E-545.01
CGTTTTT218450.044.320211
AGGGCGA18100.040.7734836
TCCGCGG451.9290383E-840.02
ACGGGTC1300.039.8076935
TAGGGCG6800.039.7058835
CGAGGGT2650.039.0566024
GGCGATT10400.038.942318
GTTTTTT251450.038.691592
TGGGCGA9150.038.606566
CGGCGAT356.249782E-638.5714266
TCGTTAG700.038.5714261
CGACGGT356.249782E-638.57142628
TACGGGC2050.038.4146354
TACGGGA4400.038.3522724
ACGCGAG659.094947E-1238.0769271
Produced by FastQC (version 0.11.5)