##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546975_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1582859
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.669465821023856	31.0	31.0	33.0	30.0	34.0
2	32.031421623783295	33.0	31.0	34.0	30.0	34.0
3	31.87684563185982	33.0	31.0	34.0	30.0	34.0
4	35.72349463849907	37.0	35.0	37.0	33.0	37.0
5	35.73701447823211	37.0	35.0	37.0	33.0	37.0
6	35.817264835339095	37.0	35.0	37.0	35.0	37.0
7	36.1932819031891	37.0	35.0	37.0	35.0	37.0
8	36.147980963560244	37.0	35.0	37.0	35.0	37.0
9	37.94207633149889	39.0	38.0	39.0	35.0	39.0
10	37.42157324183645	39.0	37.0	39.0	34.0	39.0
11	37.212462386100086	39.0	37.0	39.0	34.0	39.0
12	37.045928285463205	39.0	37.0	39.0	33.0	39.0
13	37.026996719227675	39.0	37.0	39.0	33.0	39.0
14	38.264627487350424	40.0	37.0	41.0	33.0	41.0
15	38.30565893740378	40.0	37.0	41.0	33.0	41.0
16	38.32986134583055	40.0	37.0	41.0	33.0	41.0
17	38.294297217882324	40.0	37.0	41.0	33.0	41.0
18	38.024391307122116	39.0	37.0	41.0	33.0	41.0
19	37.789599705343306	39.0	37.0	41.0	34.0	41.0
20	37.47775323007293	39.0	35.0	41.0	33.0	41.0
21	37.43250409543743	39.0	35.0	41.0	33.0	41.0
22	37.363521956156546	39.0	35.0	41.0	33.0	41.0
23	37.25884933528508	39.0	35.0	41.0	33.0	41.0
24	37.16002625628688	39.0	35.0	41.0	33.0	41.0
25	37.07549124716731	38.0	35.0	41.0	33.0	41.0
26	36.953763411649426	38.0	35.0	41.0	33.0	41.0
27	36.87850528695228	38.0	35.0	41.0	32.0	41.0
28	36.84564765402351	38.0	35.0	41.0	32.0	41.0
29	36.8020272178381	38.0	35.0	41.0	32.0	41.0
30	36.677169602598845	38.0	35.0	41.0	31.0	41.0
31	36.445972130177104	38.0	35.0	41.0	31.0	41.0
32	36.00338564584717	38.0	35.0	41.0	30.0	41.0
33	35.47233455412011	38.0	35.0	41.0	25.0	41.0
34	34.90219406782285	38.0	35.0	41.0	21.0	41.0
35	34.51832348933165	38.0	34.0	41.0	18.0	41.0
36	34.29014650073064	38.0	34.0	41.0	16.0	41.0
37	34.17688688632405	38.0	34.0	41.0	15.0	41.0
38	34.055050386673734	38.0	34.0	41.0	15.0	41.0
39	33.95285998310652	38.0	34.0	41.0	15.0	41.0
40	33.86470178329213	38.0	33.0	41.0	14.0	41.0
41	33.809660241373365	38.0	33.0	41.0	12.0	41.0
42	33.706428683793064	38.0	33.0	40.0	12.0	41.0
43	33.60854630766227	38.0	33.0	40.0	10.0	41.0
44	33.453672121142816	38.0	33.0	40.0	10.0	41.0
45	33.391052519523214	38.0	33.0	40.0	10.0	41.0
46	33.32973246511534	38.0	33.0	40.0	10.0	41.0
47	33.302714897536674	38.0	33.0	40.0	10.0	41.0
48	33.18668055714375	37.0	33.0	40.0	10.0	41.0
49	33.10199076481228	37.0	33.0	40.0	10.0	41.0
50	33.00890477294566	37.0	33.0	40.0	10.0	41.0
51	32.65398370922489	37.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	5.0
14	9.0
15	23.0
16	87.0
17	274.0
18	629.0
19	1319.0
20	2423.0
21	4129.0
22	6668.0
23	10803.0
24	18415.0
25	33551.0
26	48912.0
27	49050.0
28	40927.0
29	35129.0
30	34496.0
31	37431.0
32	44301.0
33	56349.0
34	84702.0
35	134821.0
36	111191.0
37	137345.0
38	211828.0
39	477705.0
40	334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.672699842500183	22.60984711841042	25.271992009395657	22.44546102969374
2	39.978861035632356	23.28002683751364	25.209131072319142	11.531981054534864
3	25.483571183535613	23.208068438186853	39.13955696622378	12.168803412053759
4	21.300949737152834	26.273913216527816	39.325486350963665	13.099650695355683
5	19.767016518843434	29.936399894115645	37.845190253838155	12.451393333202768
6	18.17167543034471	38.304106682907324	34.246006751075114	9.278211135672855
7	79.46538510379004	2.4580205817448046	16.709068843150273	1.3675254713148801
8	79.44049343624417	2.1296274652385336	16.38162337896174	2.0482557195555637
9	74.24483166220112	4.545003692685198	18.492740035593823	2.7174246095198624
10	32.4949347983617	28.74393739429728	26.26033019997359	12.500797607367428
11	23.53203917721035	25.684283944432195	35.31837011382568	15.465306764531775
12	21.83820542448822	22.048394708562167	38.3908484583908	17.722551408558818
13	19.579065475825704	25.951521898033874	39.142273569534616	15.327139056605802
14	18.068254974069074	28.080517595060584	37.374017521459585	16.477209909410757
15	16.71146956235521	25.343760878258898	42.43669208691362	15.508077472472278
16	18.192207897229	24.089258740039384	41.629924080413986	16.088609282317627
17	18.61574530643601	24.307092419476405	37.1269329738151	19.950229300272483
18	19.332044105002407	24.452462285017173	38.83586598679983	17.379627623180586
19	20.840517064375284	26.728596798577765	35.56918209391993	16.861704043127027
20	21.98850308208122	25.16598130345154	35.35305418865483	17.492461425812404
21	20.43972331079395	26.32641315493041	36.588034689128975	16.645828845146664
22	20.22372175917122	24.31265197974046	36.00516533689987	19.458460924188447
23	17.96957277938212	27.378433581260236	35.33782857474987	19.314165064607778
24	17.683887193995172	25.784166498721618	39.22813086952154	17.30381543776167
25	17.911323750251917	26.89127711312252	37.29776309829239	17.89963603833317
26	17.424862227147205	28.650119814841375	36.11559842032676	17.809419537684658
27	18.039951758179345	27.200780360095244	36.13455146668149	18.624716415043917
28	17.628797005924092	26.84762192968546	38.407148078255865	17.116432986134583
29	17.404582467547648	25.337695903425384	38.13536139352905	19.12236023549792
30	18.446873663415378	26.166259913232953	37.330109630737795	18.05675679261387
31	19.710536440706342	26.253128042358796	34.8602118066107	19.176123710324166
32	20.03810825853724	26.868280750212115	35.01423689665346	18.07937409459718
33	20.212413108179568	27.002215611118867	33.50235238893673	19.28301889176484
34	18.510555899167265	27.872349969264476	33.96499625045566	19.652097881112596
35	19.81755797578938	27.51167349713398	32.754970594348585	19.915797932728058
36	21.193359610679156	27.99074333216035	31.64438525478264	19.17151180237785
37	20.846518862387615	28.961518366449567	31.800874240851524	18.391088530311293
38	20.563802587596243	28.888865022089778	31.780784011715507	18.766548378598472
39	20.794018923985018	29.231851984289186	30.710631837706327	19.26349725401947
40	21.499261778844485	27.760147934844483	31.188374959487863	19.552215326823173
41	19.48265764670132	27.441357695157937	31.437481165410187	21.63850349273056
42	20.635066041890024	28.75088684462735	29.995091160994125	20.618955952488506
43	20.557674435941546	28.19575211689734	31.1706222727356	20.07595117442552
44	20.445851462448644	29.867726689490347	29.983087564969463	19.703334283091543
45	19.446899565912062	30.04569579476125	29.519116990205696	20.98828764912099
46	20.47819799489405	29.29875623792138	29.99831317887443	20.22473258831014
47	20.57384770216425	28.357927016872637	30.495577938401336	20.57264734256178
48	20.355319077694222	27.445148304428884	31.155902073400092	21.043630544476798
49	20.506185326677866	27.053894250846096	30.801480106566665	21.638440315909378
50	19.281313117592912	28.074199912942337	31.561434088570113	21.083052880894634
51	18.921837005064887	28.616446569151137	30.476814422510156	21.984902003273824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	1155.0
2	1494.0
3	24574.5
4	47655.0
5	33410.0
6	19165.0
7	19185.0
8	19205.0
9	20601.0
10	21997.0
11	22591.5
12	23186.0
13	23427.0
14	23668.0
15	23358.5
16	23049.0
17	21894.0
18	20739.0
19	19976.5
20	19214.0
21	18360.0
22	17506.0
23	17311.0
24	17116.0
25	17666.5
26	19494.5
27	20772.0
28	22389.5
29	24007.0
30	27055.5
31	30104.0
32	33597.0
33	37090.0
34	41460.0
35	45830.0
36	50282.0
37	54734.0
38	60572.5
39	66411.0
40	74089.0
41	81767.0
42	89917.5
43	98068.0
44	106242.0
45	114416.0
46	122195.0
47	129974.0
48	133017.5
49	136061.0
50	129158.5
51	122256.0
52	108803.0
53	95350.0
54	84260.5
55	73171.0
56	64593.0
57	56015.0
58	49280.5
59	42546.0
60	36617.0
61	30688.0
62	25465.0
63	20242.0
64	16176.5
65	12111.0
66	9907.5
67	7704.0
68	5981.0
69	4258.0
70	3327.5
71	2397.0
72	1926.0
73	1455.0
74	1244.0
75	840.5
76	648.0
77	474.0
78	300.0
79	263.5
80	227.0
81	179.0
82	131.0
83	90.5
84	50.0
85	29.0
86	8.0
87	6.5
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1582859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.38055850607592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9703051407583	28.774021826732376
2	8.396174255973799	6.4449971451722154
3	3.8338430379877324	4.414351110678001
4	2.361731325875372	3.6257826931356782
5	1.6638301938154096	3.1929366048953987
6	1.275288123705262	2.9367762266384156
7	0.9637226861473477	2.589175045751764
8	0.791899319562238	2.4314830532304152
9	0.6409613309948256	2.2140408477941276
>10	4.971977502947315	36.7348232931701
>50	0.11235268653157489	2.7300374599777233
>100	0.014762819638890765	0.9343855877162759
>500	0.0021563415160770747	0.5214616905791818
>1k	8.293621215681057E-4	0.4717410727861967
>5k	0.0	0.0
>10k+	1.6587242431362112E-4	1.9839863417421097
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31164	1.9688424553292492	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	1808	0.11422369269783349	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	1628	0.10285186488499606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2635364236486004E-4	0.0	0.0	0.08137174568296987	0.0
2	1.2635364236486004E-4	0.0	0.0	0.2375448476459369	0.0
3	1.2635364236486004E-4	0.0	0.0	0.43364570059619967	0.0
4	1.2635364236486004E-4	0.0	0.0	0.6212176826868344	0.0
5	1.2635364236486004E-4	0.0	0.0	1.0247912164001973	0.0
6	1.2635364236486004E-4	0.0	0.0	1.9105934261990487	0.0
7	1.2635364236486004E-4	0.0	0.0	2.3899791453313277	0.0
8	1.2635364236486004E-4	0.0	0.0	3.4136963557714237	0.0
9	1.2635364236486004E-4	0.0	0.0	4.055320151700183	0.0
10	1.2635364236486004E-4	0.0	0.0	4.8317001072110655	0.0
11	1.2635364236486004E-4	0.0	0.0	5.402376332951956	0.0
12	1.2635364236486004E-4	0.0	0.0	5.90892808519268	0.0
13	1.2635364236486004E-4	0.0	0.0	6.274532349375402	0.0
14	1.2635364236486004E-4	0.0	0.0	6.506391283114921	0.0
15	1.2635364236486004E-4	0.0	0.0	6.687961467193224	0.0
16	1.2635364236486004E-4	0.0	0.0	6.930497283712573	0.0
17	1.8953046354729005E-4	0.0	0.0	7.19558722539405	0.0
18	1.8953046354729005E-4	0.0	0.0	7.555442398849171	0.0
19	1.8953046354729005E-4	0.0	0.0	7.76885370080342	0.0
20	1.8953046354729005E-4	0.0	0.0	7.998817329907465	0.0
21	1.8953046354729005E-4	0.0	0.0	8.248428950399246	0.0
22	1.8953046354729005E-4	0.0	0.0	8.519015275523593	0.0
23	1.8953046354729005E-4	0.0	0.0	8.828265815211589	0.0
24	1.8953046354729005E-4	0.0	0.0	9.08880702576793	0.0
25	1.8953046354729005E-4	0.0	0.0	9.318075709838968	0.0
26	1.8953046354729005E-4	0.0	0.0	9.542353425036595	0.0
27	1.8953046354729005E-4	0.0	0.0	9.778887443543614	0.0
28	1.8953046354729005E-4	0.0	0.0	10.014473809732895	0.0
29	1.8953046354729005E-4	0.0	0.0	10.27255112426312	0.0
30	1.8953046354729005E-4	0.0	0.0	10.624193310964527	0.0
31	1.8953046354729005E-4	0.0	0.0	10.888967368540092	0.0
32	1.8953046354729005E-4	0.0	0.0	11.148687280421061	0.0
33	1.8953046354729005E-4	0.0	0.0	11.41099744197051	0.0
34	1.8953046354729005E-4	0.0	0.0	11.689923107490939	0.0
35	1.8953046354729005E-4	0.0	0.0	11.969543718044374	0.0
36	1.8953046354729005E-4	0.0	0.0	12.256682370318519	0.0
37	1.8953046354729005E-4	0.0	0.0	12.53516579809067	0.0
38	1.8953046354729005E-4	0.0	0.0	12.820156438444611	0.0
39	1.8953046354729005E-4	0.0	0.0	13.113739126479365	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGT	20	7.0340926E-4	45.000004	17
CGACGTC	20	7.0340926E-4	45.000004	18
CAACGCG	25	3.8914746E-5	45.0	1
ATCCGCG	25	3.8914746E-5	45.0	1
TTACGCG	25	3.8914746E-5	45.0	1
CGTTTTT	21845	0.0	44.32021	1
AGGGCGA	1810	0.0	40.773483	6
TCCGCGG	45	1.9290383E-8	40.0	2
ACGGGTC	130	0.0	39.807693	5
TAGGGCG	680	0.0	39.705883	5
CGAGGGT	265	0.0	39.056602	4
GGCGATT	1040	0.0	38.94231	8
GTTTTTT	25145	0.0	38.69159	2
TGGGCGA	915	0.0	38.60656	6
CGGCGAT	35	6.249782E-6	38.571426	6
TCGTTAG	70	0.0	38.571426	1
CGACGGT	35	6.249782E-6	38.571426	28
TACGGGC	205	0.0	38.414635	4
TACGGGA	440	0.0	38.352272	4
ACGCGAG	65	9.094947E-12	38.076927	1
>>END_MODULE