FastQCFastQC Report
Thu 26 May 2016
SRR1546974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546974_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2538445
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148950.5867765502108574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG351.212702E-745.01
CGTTTTT88000.041.2670441
TACGGGT1600.040.781254
TTAATCG3650.040.06849320
TAATCGT3750.039.021
ACGTAAG1100.038.863641
CGTCATA7100.038.66197238
TACGGGA6200.037.7419364
GCGTAAG2150.037.674421
GTCATAC7300.037.60273739
ACAACGA8800.037.58522813
TATGGGC9700.037.577324
CACGTAA301.14031056E-437.50000434
TACGTTA301.14031056E-437.50000430
AACGGGA8400.037.54
ACGGGAT5350.037.4299055
CGTAAGG5050.037.4257432
TTCGAAT850.037.05882316
ACTAATC10350.036.95652410
CACAACG8950.036.95530712
Produced by FastQC (version 0.11.5)