##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546974_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2538445
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63670278457875	31.0	31.0	33.0	30.0	34.0
2	32.01747053806562	33.0	31.0	34.0	30.0	34.0
3	32.114097803970544	33.0	31.0	34.0	30.0	34.0
4	35.79361183716803	37.0	35.0	37.0	35.0	37.0
5	35.7447559431069	37.0	35.0	37.0	33.0	37.0
6	35.79347907872733	37.0	35.0	37.0	35.0	37.0
7	36.116978307585946	37.0	35.0	37.0	35.0	37.0
8	36.044166408962965	37.0	35.0	37.0	35.0	37.0
9	37.80539700485927	39.0	38.0	39.0	35.0	39.0
10	37.35809718154224	39.0	37.0	39.0	34.0	39.0
11	37.09643502222818	39.0	37.0	39.0	33.0	39.0
12	36.63793503503129	39.0	35.0	39.0	33.0	39.0
13	36.48420824559918	39.0	35.0	39.0	32.0	39.0
14	37.56480364947832	40.0	35.0	41.0	33.0	41.0
15	37.663812294534644	40.0	35.0	41.0	33.0	41.0
16	37.738836177266	40.0	35.0	41.0	33.0	41.0
17	37.6845982481401	40.0	35.0	41.0	33.0	41.0
18	37.607847717795735	39.0	36.0	41.0	33.0	41.0
19	37.53614122031401	39.0	35.0	41.0	33.0	41.0
20	37.404339270695246	39.0	35.0	41.0	32.0	41.0
21	37.320472966717816	39.0	35.0	41.0	32.0	41.0
22	37.251782882827875	39.0	35.0	41.0	32.0	41.0
23	37.162210329552146	39.0	35.0	41.0	32.0	41.0
24	37.056100092773335	39.0	35.0	41.0	32.0	41.0
25	36.93600570427959	39.0	35.0	41.0	32.0	41.0
26	36.78830071165615	38.0	35.0	41.0	31.0	41.0
27	36.687867572470545	38.0	35.0	41.0	31.0	41.0
28	36.721002424712765	38.0	35.0	41.0	31.0	41.0
29	36.70802637047484	38.0	35.0	41.0	31.0	41.0
30	36.61012824780525	38.0	35.0	41.0	31.0	41.0
31	36.48456161153777	38.0	35.0	41.0	31.0	41.0
32	36.39098621400109	38.0	35.0	41.0	31.0	41.0
33	36.241419451672186	38.0	35.0	41.0	30.0	41.0
34	36.05417647418006	38.0	35.0	41.0	30.0	41.0
35	35.926954099852466	38.0	35.0	41.0	30.0	41.0
36	35.79218458544503	38.0	35.0	41.0	29.0	41.0
37	35.724690115405295	38.0	35.0	41.0	29.0	41.0
38	35.58314755687044	38.0	35.0	41.0	29.0	41.0
39	35.49552619812523	38.0	35.0	40.0	28.0	41.0
40	35.3263119744568	38.0	35.0	40.0	27.0	41.0
41	35.26356450504147	37.0	34.0	40.0	27.0	41.0
42	35.17256982128823	37.0	34.0	40.0	27.0	41.0
43	35.05821359139158	37.0	34.0	40.0	26.0	41.0
44	34.925140785008146	37.0	34.0	40.0	26.0	41.0
45	34.8085966014627	37.0	34.0	40.0	26.0	41.0
46	34.737152075384735	36.0	34.0	40.0	26.0	41.0
47	34.66303268339475	36.0	34.0	40.0	26.0	41.0
48	34.54351581381515	36.0	34.0	40.0	25.0	41.0
49	34.4574418590909	36.0	34.0	40.0	25.0	41.0
50	34.33962445512903	35.0	34.0	40.0	24.0	41.0
51	33.98201576161784	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	15.0
14	32.0
15	66.0
16	192.0
17	491.0
18	1079.0
19	2069.0
20	3496.0
21	5547.0
22	8573.0
23	12396.0
24	18398.0
25	26055.0
26	34069.0
27	40124.0
28	43725.0
29	49095.0
30	57046.0
31	67720.0
32	82873.0
33	108144.0
34	189147.0
35	293873.0
36	194367.0
37	252118.0
38	363474.0
39	683717.0
40	537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.20808999210146	24.666242522489163	26.821262623377695	13.304404862031676
2	32.794092446359876	25.811549984340807	27.48127298405126	13.913084585248056
3	30.362958425335197	25.635773081551893	30.187378493526552	13.813889999586362
4	25.886280774253528	28.967261453370075	29.31578190585181	15.830675866524585
5	24.034714165561986	32.44557199387814	28.05457671921196	15.46513712134791
6	22.543289297187847	40.76905349534853	25.929732572500093	10.757924634963532
7	87.37191469580786	3.659208688783881	7.105334171116569	1.8635424442916826
8	86.8182292702816	3.3978281979715925	6.8594749935492	2.924467538197597
9	81.33077533686962	5.2416341500406745	9.636253690743743	3.7913368223459636
10	45.79441350905771	25.052975345142396	15.316581608031687	13.836029537768201
11	36.048328799717936	25.776134602089073	22.059292204479515	16.116244393713476
12	32.073375629568496	21.670983613984152	26.63457352828208	19.621067228165273
13	24.273088445879267	28.22944755549165	28.68263838688646	18.814825611742624
14	20.995254969085405	29.34276693014818	29.748921091455593	19.91305700931082
15	20.361678113963467	23.7249576019965	36.63758718428014	19.275777099759893
16	22.81806381465819	21.489455158571488	35.46560985170055	20.22687117506978
17	22.677387140552582	22.45780389175263	28.457027826090382	26.40778114160441
18	24.791791825310376	22.449334139601213	30.19084518277922	22.568028852309187
19	27.257513950469676	25.493757004780488	25.995560274104818	21.25316877064502
20	30.03736539495636	23.96029852921769	25.114903021337863	20.887433054488085
21	26.983251557548027	26.178270555399074	26.390959819889737	20.447518067163166
22	25.91590520968546	23.30095786987703	25.87205946947836	24.91107745095915
23	25.09520592331132	26.757325843183523	24.98135669671787	23.166111536787284
24	24.206118312589005	23.61540234277284	31.092696513022737	21.08578283161542
25	23.842628065607094	24.149233093488338	28.4687672965142	23.539371544390363
26	22.21422957755634	27.575897842970793	27.00413048145617	23.2057420980167
27	22.625544378546707	26.316583577741493	27.005627460906183	24.052244582805614
28	21.656132002072134	26.925972396486824	29.503652826829025	21.914242774612017
29	22.943534329087296	25.016929655753817	28.59817723054862	23.441358784610262
30	24.590960213831696	25.7230312258095	27.982052004278206	21.70395655608059
31	26.820986864005324	26.396908343493752	24.999832574666776	21.782272217834148
32	28.221883869849457	25.659764146948227	24.957208054537325	21.16114392866499
33	26.62504013283723	27.18069526816614	24.313861438794223	21.88040316020241
34	23.409134332238832	26.497048389860723	28.089519371111056	22.004297906789393
35	23.97404710364022	27.37120560027891	27.45720313026282	21.19754416581805
36	26.68850418267877	28.096886085772983	24.954489855009662	20.26011987653859
37	25.631597296770263	28.650019992554498	25.22934316087211	20.489039549803127
38	24.52048399709271	28.37780609782761	25.868947327990167	21.232762577089517
39	25.26534945606464	27.388814805914645	24.793131227976183	22.552704510044535
40	26.55570634778378	24.47021700292896	26.890478225842983	22.083598423444272
41	21.991337216287924	24.605693643155554	27.083706757483423	26.3192623830731
42	24.717336794770027	25.720116055301574	24.87964088250878	24.68290626741962
43	23.372103787948923	24.62216041710575	27.103561432294182	24.90217436265115
44	23.434346617712812	26.22916785669967	26.551609351394255	23.784876174193258
45	22.487782874949033	26.381662789621206	25.953566061112216	25.176988274317548
46	24.238027611392013	26.268601446948818	26.246264937786716	23.24710600387245
47	23.769315466752282	25.289655675029397	27.742417109687228	23.19861174853109
48	24.278997575287235	23.72172727792014	28.86641231147415	23.13286283531847
49	24.188942443109855	24.237830640411747	27.909093953187874	23.66413296329052
50	22.50019204670576	25.9673540297308	27.952388174650228	23.580065748913213
51	22.298848310678387	26.588521713096007	26.302756214926852	24.809873761298746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1406.0
1	2816.5
2	4227.0
3	14933.0
4	25639.0
5	17837.5
6	10036.0
7	10115.5
8	10195.0
9	10769.0
10	11343.0
11	11434.5
12	11526.0
13	11653.0
14	11780.0
15	11499.0
16	11218.0
17	11069.5
18	10921.0
19	10960.0
20	10999.0
21	10942.0
22	10885.0
23	11279.0
24	11673.0
25	13310.5
26	16043.0
27	17138.0
28	21120.0
29	25102.0
30	27574.0
31	30046.0
32	34855.5
33	39665.0
34	45803.5
35	51942.0
36	56221.5
37	60501.0
38	67809.5
39	75118.0
40	92312.0
41	109506.0
42	128864.5
43	148223.0
44	166604.5
45	184986.0
46	192278.5
47	199571.0
48	191276.5
49	182982.0
50	174009.5
51	165037.0
52	159485.5
53	153934.0
54	146219.5
55	138505.0
56	138089.5
57	137674.0
58	134848.0
59	132022.0
60	128508.5
61	124995.0
62	116181.5
63	107368.0
64	97243.0
65	87118.0
66	74019.0
67	60920.0
68	53125.0
69	45330.0
70	39678.0
71	34026.0
72	28900.5
73	23775.0
74	21729.0
75	16086.0
76	12489.0
77	9935.0
78	7381.0
79	5532.0
80	3683.0
81	2684.0
82	1685.0
83	1220.5
84	756.0
85	527.5
86	299.0
87	200.0
88	101.0
89	77.0
90	53.0
91	34.0
92	15.0
93	14.0
94	13.0
95	9.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2538445.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.58804955163611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44341582475924	16.934403478244988
2	7.1885821259331895	3.103749342813027
3	2.4171911478358905	1.565473268257721
4	1.2666749236796024	1.093801640728406
5	0.8412421792936523	0.9080388925758859
6	0.6145919892729105	0.796070539107732
7	0.4928339958838457	0.74475273067099
8	0.3917939003392493	0.6766452907641997
9	0.35693040904460327	0.6934888221246578
>10	5.5266139787143524	30.692034044479637
>50	2.1463338530055363	32.360854405437614
>100	0.30806009026121656	8.657185325470342
>500	0.003515356695513323	0.472872680838404
>1k	0.002035206507928766	0.7056942752810982
>5k	0.0	0.0
>10k+	1.8501877344806963E-4	0.5949352632053406
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14895	0.5867765502108574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.57576784212382E-4	0.0	0.0	0.0735095698350762	0.0
2	1.57576784212382E-4	0.0	0.0	0.23573486918172346	0.0
3	1.57576784212382E-4	0.0	0.0	0.3673508781951155	0.0
4	1.57576784212382E-4	0.0	0.0	0.6489405915826422	0.0
5	1.57576784212382E-4	0.0	0.0	1.0802676441679848	0.0
6	1.57576784212382E-4	0.0	0.0	1.9439066042399973	0.0
7	1.57576784212382E-4	0.0	0.0	2.3086574654956085	0.0
8	1.57576784212382E-4	0.0	0.0	3.2783849955386075	0.0
9	1.57576784212382E-4	0.0	0.0	3.749657762921789	0.0
10	1.57576784212382E-4	0.0	0.0	4.441341057222039	0.0
11	1.57576784212382E-4	0.0	0.0	5.013344783912986	0.0
12	1.57576784212382E-4	0.0	0.0	5.455465846216877	0.0
13	1.57576784212382E-4	0.0	0.0	5.690412831477539	0.0
14	1.57576784212382E-4	0.0	0.0	5.804183269678878	0.0
15	1.57576784212382E-4	0.0	0.0	5.933553809517244	0.0
16	1.57576784212382E-4	0.0	0.0	6.165309864897605	0.0
17	1.57576784212382E-4	0.0	0.0	6.402659896117505	0.0
18	1.57576784212382E-4	0.0	0.0	6.7147801114461805	0.0
19	1.57576784212382E-4	0.0	0.0	6.8789751205954826	0.0
20	1.57576784212382E-4	0.0	0.0	7.063891476868713	0.0
21	1.57576784212382E-4	0.0	0.0	7.284932310922632	0.0
22	1.57576784212382E-4	0.0	0.0	7.513930772579276	0.0
23	1.57576784212382E-4	0.0	0.0	7.77003244112045	0.0
24	1.57576784212382E-4	0.0	0.0	7.983273224355855	0.0
25	1.57576784212382E-4	0.0	0.0	8.161847115064537	0.0
26	1.57576784212382E-4	0.0	0.0	8.330572456759946	0.0
27	1.57576784212382E-4	0.0	0.0	8.504143284569885	0.0
28	1.57576784212382E-4	0.0	0.0	8.67869896728115	0.0
29	1.57576784212382E-4	0.0	0.0	8.867712319943903	0.0
30	1.57576784212382E-4	0.0	0.0	9.125310967935095	0.0
31	1.57576784212382E-4	0.0	0.0	9.327166828511155	0.0
32	1.57576784212382E-4	0.0	0.0	9.55553498303095	0.0
33	1.57576784212382E-4	0.0	0.0	9.7591635824294	0.0
34	1.57576784212382E-4	0.0	0.0	9.961570961750205	0.0
35	1.57576784212382E-4	0.0	0.0	10.194075506855574	0.0
36	1.57576784212382E-4	0.0	0.0	10.407867808835725	0.0
37	1.57576784212382E-4	0.0	0.0	10.622408600540883	0.0
38	1.57576784212382E-4	0.0	0.0	10.865982914737172	0.0
39	1.57576784212382E-4	0.0	0.0	11.173966739480273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	35	1.212702E-7	45.0	1
CGTTTTT	8800	0.0	41.267044	1
TACGGGT	160	0.0	40.78125	4
TTAATCG	365	0.0	40.068493	20
TAATCGT	375	0.0	39.0	21
ACGTAAG	110	0.0	38.86364	1
CGTCATA	710	0.0	38.661972	38
TACGGGA	620	0.0	37.741936	4
GCGTAAG	215	0.0	37.67442	1
GTCATAC	730	0.0	37.602737	39
ACAACGA	880	0.0	37.585228	13
TATGGGC	970	0.0	37.57732	4
CACGTAA	30	1.14031056E-4	37.500004	34
TACGTTA	30	1.14031056E-4	37.500004	30
AACGGGA	840	0.0	37.5	4
ACGGGAT	535	0.0	37.429905	5
CGTAAGG	505	0.0	37.425743	2
TTCGAAT	85	0.0	37.058823	16
ACTAATC	1035	0.0	36.956524	10
CACAACG	895	0.0	36.955307	12
>>END_MODULE