FastQCFastQC Report
Thu 26 May 2016
SRR1546973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546973_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences146621
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.4242229967057925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA302.1525193E-645.0000044
CGGGATA302.1525193E-645.0000046
CGGGTAT351.202261E-745.06
GTATTGG253.8741186E-545.02
CACGGGG453.8016879E-1045.04
TGGGTAC253.8741186E-545.06
TACGGGA207.013143E-445.04
ACGGCGA351.202261E-745.016
CGAGGGC207.013143E-445.04
TAGGGAC800.045.05
GCGATAT253.8741186E-545.09
CGATGAG253.8741186E-545.010
CGACTGG253.8741186E-545.02
GACGAAG207.013143E-445.01
AACATAC551.8189894E-1244.99999616
ACATACG551.8189894E-1244.99999617
ACGAAAA551.8189894E-1244.99999621
TGTTAGG750.042.0000042
GGCGATT750.042.0000048
CGTTTTT4350.041.896551
Produced by FastQC (version 0.11.5)