##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546973_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	146621
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617387686620607	31.0	31.0	33.0	30.0	34.0
2	32.01375655601858	31.0	31.0	34.0	30.0	34.0
3	32.12683039946529	33.0	31.0	34.0	30.0	34.0
4	35.819575640597186	37.0	35.0	37.0	35.0	37.0
5	35.77659407588272	37.0	35.0	37.0	35.0	37.0
6	35.80626240443047	37.0	35.0	37.0	35.0	37.0
7	36.082668921914326	37.0	35.0	37.0	35.0	37.0
8	36.01185369080827	37.0	35.0	37.0	35.0	37.0
9	37.79466106492249	39.0	38.0	39.0	35.0	39.0
10	37.35181863443845	39.0	37.0	39.0	34.0	39.0
11	37.101465683633315	39.0	37.0	39.0	33.0	39.0
12	36.61477550964732	39.0	35.0	39.0	33.0	39.0
13	36.45654442405931	39.0	35.0	39.0	32.0	39.0
14	37.52866233349929	40.0	35.0	41.0	33.0	41.0
15	37.64576697744525	40.0	35.0	41.0	33.0	41.0
16	37.751918210897486	40.0	35.0	41.0	33.0	41.0
17	37.71442017173529	40.0	35.0	41.0	33.0	41.0
18	37.64380954979164	39.0	36.0	41.0	33.0	41.0
19	37.56393695309676	39.0	35.0	41.0	33.0	41.0
20	37.41494056103832	39.0	35.0	41.0	33.0	41.0
21	37.33704585291329	39.0	35.0	41.0	32.0	41.0
22	37.27871860101895	39.0	35.0	41.0	32.0	41.0
23	37.17202856343907	39.0	35.0	41.0	32.0	41.0
24	37.051902524195036	39.0	35.0	41.0	32.0	41.0
25	36.9348592629978	39.0	35.0	41.0	32.0	41.0
26	36.80068339460241	38.0	35.0	41.0	31.0	41.0
27	36.68075514421536	38.0	35.0	41.0	31.0	41.0
28	36.71347896958826	38.0	35.0	41.0	31.0	41.0
29	36.71695732534903	38.0	35.0	41.0	31.0	41.0
30	36.62150033078481	38.0	35.0	41.0	31.0	41.0
31	36.5167472599423	38.0	35.0	41.0	31.0	41.0
32	36.43686102263659	38.0	35.0	41.0	31.0	41.0
33	36.316025671629575	38.0	35.0	41.0	30.0	41.0
34	36.14036188540523	38.0	35.0	41.0	30.0	41.0
35	36.01625960810525	38.0	35.0	41.0	30.0	41.0
36	35.87660021415759	38.0	35.0	41.0	29.0	41.0
37	35.79039155373378	38.0	35.0	41.0	29.0	41.0
38	35.65902565116866	38.0	35.0	41.0	29.0	41.0
39	35.55444990826689	38.0	35.0	40.0	28.0	41.0
40	35.36312670081366	38.0	35.0	40.0	27.0	41.0
41	35.26989312581417	37.0	34.0	40.0	27.0	41.0
42	35.17217178985275	37.0	34.0	40.0	27.0	41.0
43	35.0532734055831	37.0	34.0	40.0	27.0	41.0
44	34.842444124647905	37.0	34.0	40.0	26.0	41.0
45	34.742860845308655	36.0	34.0	40.0	26.0	41.0
46	34.71030752757108	36.0	34.0	40.0	26.0	41.0
47	34.66884689096378	36.0	34.0	40.0	26.0	41.0
48	34.56345271141242	36.0	34.0	40.0	26.0	41.0
49	34.47752368351055	36.0	34.0	40.0	26.0	41.0
50	34.377019662940505	35.0	34.0	40.0	26.0	41.0
51	34.00322600446048	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	4.0
16	8.0
17	31.0
18	66.0
19	142.0
20	193.0
21	326.0
22	489.0
23	709.0
24	977.0
25	1410.0
26	1816.0
27	2244.0
28	2482.0
29	2841.0
30	3299.0
31	3885.0
32	4781.0
33	6247.0
34	11029.0
35	17488.0
36	11195.0
37	14985.0
38	21495.0
39	38448.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.300536758036024	24.39759652437236	27.36920359293689	12.932663124654722
2	33.023918811084364	25.441103252603654	27.58949945778572	13.945478478526269
3	30.858471842369102	25.54545392542678	29.8640713131134	13.732002919090716
4	25.62934368201008	29.238649306715953	29.377783537146797	15.754223474127171
5	23.937225908976203	32.56900444001882	28.442719664986598	15.051049986018375
6	22.631137422333772	41.9967126127908	25.353803343313714	10.018346621561713
7	88.7185328158995	3.6290845104043763	6.027785924253688	1.62459674944244
8	88.57871655492733	3.00366250400693	6.208524017705512	2.209096923360228
9	83.425975815197	5.1240954569945645	8.166633701857169	3.283295025951262
10	47.628238792533125	25.139645753336836	14.289221871355398	12.942893582774637
11	37.203401968340145	25.101452043022487	20.971757115283623	16.72338887335375
12	31.78603337857469	22.226693311326482	26.91019703862339	19.077076271475438
13	24.35735672243403	28.60640699490523	29.03267608323501	18.00356019942573
14	21.812700772740605	29.83269790821233	29.776771403823467	18.577829915223603
15	20.33133043697697	22.840520798521357	37.445522810511456	19.38262595399022
16	23.101738495849844	20.618465294875904	35.08910729022446	21.190688919049794
17	23.36909446805028	21.18386861363652	28.2947190375185	27.1523178807947
18	25.322429938412643	22.48995710027895	30.111648399615337	22.075964561693073
19	28.665743652000735	24.340987989442166	25.475204779670037	21.518063578887062
20	29.9677399553952	23.69237694463958	24.840234345694	21.499648754271217
21	26.930657954863218	25.29787683892485	26.874731450474354	20.896733755737582
22	26.532352118727875	22.870530142339774	25.868054371474752	24.729063367457595
23	24.960271720967665	25.87214655472272	25.259001098069174	23.908580626240443
24	23.414108483777905	24.280287271264005	31.054214607730135	21.251389637227955
25	23.942682153306823	24.08386247536165	29.270704742158355	22.702750629173174
26	22.0889231419783	27.693850130608848	26.45255454539254	23.76467218202031
27	22.774363836012576	26.52894196602124	27.044557055264935	23.65213714270125
28	21.69743761125623	26.464831095136443	30.33330832554682	21.50442296806051
29	23.101738495849844	24.294609912631888	29.498502942961785	23.10514864855648
30	24.212766247672572	25.48202508508331	28.37178848868852	21.933420178555597
31	26.567135676335585	25.859187974437496	24.73588367287087	22.83779267635605
32	28.024634943152755	25.273323739437053	24.60015959514667	22.10188172226352
33	26.873367389391696	25.42337045852913	24.653357977370227	23.049904174708942
34	23.465942804918804	26.95043684056172	26.38366946071845	23.199950893801024
35	24.523772174517973	26.257493810572836	27.01727583361183	22.201458181297358
36	28.20537303660458	26.257493810572836	23.786497159342794	21.75063599347979
37	25.115092653849043	27.813205475341185	25.092585645985228	21.979116224824548
38	25.22830972370943	27.894367109759177	24.113871819180062	22.763451347351335
39	24.64653767195695	27.30918490530006	24.7563445891107	23.28793283363229
40	26.258175841114166	24.60902599218393	25.20102850205632	23.931769664645582
41	23.125609564796314	24.080452322655006	26.331835139577553	26.46210297297113
42	24.95345141555439	25.19011601339508	24.745432100449456	25.111000470601073
43	23.928359511938947	24.724289153668302	26.94293450460712	24.40441682978564
44	23.81105025883059	26.127908007720585	26.824261190416106	23.23678054303272
45	22.217144883747896	27.25666855361783	25.684588155857618	24.841598406776654
46	23.908580626240443	26.104718969315442	27.03773674985166	22.948963654592454
47	24.238001377701693	25.381084564966823	27.371931715102203	23.008982342229285
48	24.54286902967515	24.34985438647943	27.76546333744825	23.341813246397177
49	24.313024737247733	23.86902285484344	28.087381752954897	23.73057065495393
50	22.884852783707654	26.83517367907735	26.974989940049515	23.30498359716548
51	22.999433914650698	27.426494158408417	25.612292918476893	23.961779008464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	149.5
2	213.0
3	693.0
4	1173.0
5	847.0
6	521.0
7	532.5
8	544.0
9	581.0
10	618.0
11	623.0
12	628.0
13	631.0
14	634.0
15	651.5
16	669.0
17	611.0
18	553.0
19	579.0
20	605.0
21	618.5
22	632.0
23	645.0
24	658.0
25	706.0
26	953.0
27	1152.0
28	1327.5
29	1503.0
30	1703.0
31	1903.0
32	2173.0
33	2443.0
34	2679.5
35	2916.0
36	3182.0
37	3448.0
38	4023.0
39	4598.0
40	5373.5
41	6149.0
42	7430.5
43	8712.0
44	8773.0
45	8834.0
46	9061.5
47	9289.0
48	9604.0
49	9919.0
50	10339.0
51	10759.0
52	10625.0
53	10491.0
54	9823.5
55	9156.0
56	8601.5
57	8047.0
58	7686.0
59	7325.0
60	7165.5
61	7006.0
62	6622.0
63	6238.0
64	5727.0
65	5216.0
66	4634.0
67	4052.0
68	3464.0
69	2876.0
70	2530.0
71	2184.0
72	1853.0
73	1522.0
74	1300.5
75	889.5
76	700.0
77	563.5
78	427.0
79	312.0
80	197.0
81	148.0
82	99.0
83	75.0
84	51.0
85	33.0
86	15.0
87	14.5
88	14.0
89	12.5
90	11.0
91	6.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	146621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.898070535598585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.61233480176212	28.56071094863628
2	10.659273887285432	9.571616616992108
3	7.156311711985416	9.639137640583545
4	5.307610511924654	9.532058845595106
5	4.033115600789913	9.05395543612443
6	2.9895184566307154	8.05341663199678
7	2.196566914780495	6.903513139318379
8	1.4339966580586359	5.1506946481063425
9	0.9585295457997873	3.8732514441996715
>10	1.6390703326750724	8.803650227457185
>50	0.010633449794926326	0.3573840036556837
>100	0.0015190642564180466	0.07638742062869576
>500	0.0015190642564180466	0.4242229967057925
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	622	0.4242229967057925	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.820305413276406E-4	0.0	0.0	0.05797259601284946	0.0
2	6.820305413276406E-4	0.0	0.0	0.13504204718287285	0.0
3	6.820305413276406E-4	0.0	0.0	0.2059732234809475	0.0
4	6.820305413276406E-4	0.0	0.0	0.3723886755648918	0.0
5	6.820305413276406E-4	0.0	0.0	0.6083712428642555	0.0
6	6.820305413276406E-4	0.0	0.0	1.0537371863512048	0.0
7	6.820305413276406E-4	0.0	0.0	1.2713049290347223	0.0
8	6.820305413276406E-4	0.0	0.0	1.838754339419319	0.0
9	6.820305413276406E-4	0.0	0.0	2.142939960851447	0.0
10	6.820305413276406E-4	0.0	0.0	2.6940206382441807	0.0
11	6.820305413276406E-4	0.0	0.0	3.0786858635529697	0.0
12	6.820305413276406E-4	0.0	0.0	3.4074245844728925	0.0
13	6.820305413276406E-4	0.0	0.0	3.5765681587221474	0.0
14	6.820305413276406E-4	0.0	0.0	3.6638680680120856	0.0
15	6.820305413276406E-4	0.0	0.0	3.765490618669904	0.0
16	6.820305413276406E-4	0.0	0.0	3.957141200782971	0.0
17	6.820305413276406E-4	0.0	0.0	4.139925385858779	0.0
18	6.820305413276406E-4	0.0	0.0	4.384092319654074	0.0
19	6.820305413276406E-4	0.0	0.0	4.502765633845083	0.0
20	6.820305413276406E-4	0.0	0.0	4.624167070201404	0.0
21	6.820305413276406E-4	0.0	0.0	4.779670033624106	0.0
22	6.820305413276406E-4	0.0	0.0	4.945403455166723	0.0
23	6.820305413276406E-4	0.0	0.0	5.117957182122615	0.0
24	6.820305413276406E-4	0.0	0.0	5.274142176086645	0.0
25	6.820305413276406E-4	0.0	0.0	5.392815490277655	0.0
26	6.820305413276406E-4	0.0	0.0	5.503304437972733	0.0
27	6.820305413276406E-4	0.0	0.0	5.634936332448967	0.0
28	6.820305413276406E-4	0.0	0.0	5.7467893412267	0.0
29	6.820305413276406E-4	0.0	0.0	5.875011082996297	0.0
30	6.820305413276406E-4	0.0	0.0	6.066661665109364	0.0
31	6.820305413276406E-4	0.0	0.0	6.226938842321359	0.0
32	6.820305413276406E-4	0.0	0.0	6.393354294405303	0.0
33	6.820305413276406E-4	0.0	0.0	6.542036952414729	0.0
34	6.820305413276406E-4	0.0	0.0	6.705042251792035	0.0
35	6.820305413276406E-4	0.0	0.0	6.893964711739792	0.0
36	6.820305413276406E-4	0.0	0.0	7.057652041658425	0.0
37	6.820305413276406E-4	0.0	0.0	7.198150333171919	0.0
38	6.820305413276406E-4	0.0	0.0	7.392529037450297	0.0
39	6.820305413276406E-4	0.0	0.0	7.664659223440026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGA	30	2.1525193E-6	45.000004	4
CGGGATA	30	2.1525193E-6	45.000004	6
CGGGTAT	35	1.202261E-7	45.0	6
GTATTGG	25	3.8741186E-5	45.0	2
CACGGGG	45	3.8016879E-10	45.0	4
TGGGTAC	25	3.8741186E-5	45.0	6
TACGGGA	20	7.013143E-4	45.0	4
ACGGCGA	35	1.202261E-7	45.0	16
CGAGGGC	20	7.013143E-4	45.0	4
TAGGGAC	80	0.0	45.0	5
GCGATAT	25	3.8741186E-5	45.0	9
CGATGAG	25	3.8741186E-5	45.0	10
CGACTGG	25	3.8741186E-5	45.0	2
GACGAAG	20	7.013143E-4	45.0	1
AACATAC	55	1.8189894E-12	44.999996	16
ACATACG	55	1.8189894E-12	44.999996	17
ACGAAAA	55	1.8189894E-12	44.999996	21
TGTTAGG	75	0.0	42.000004	2
GGCGATT	75	0.0	42.000004	8
CGTTTTT	435	0.0	41.89655	1
>>END_MODULE