FastQCFastQC Report
Thu 26 May 2016
SRR1546972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546972_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences623934
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.45950372956113944No Hit
GAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT8700.13943782515458367Illumina PCR Primer Index 5 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT8580.13751454480762387No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT7070.11331326710838005No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6500.10417768546032112No Hit
AAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT6360.10193385838886805Illumina PCR Primer Index 5 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT6300.10097221821538815No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT253.8887476E-545.00000428
CTAAGCG253.8887476E-545.0000041
CGGATTA253.8887476E-545.00000440
CGGTAGT253.8887476E-545.00000412
ATACCGA253.8887476E-545.00000437
CCCGACA253.8887476E-545.00000426
TACCCGT207.030804E-445.036
CACGGTA207.030804E-445.036
ATCACGA207.030804E-445.035
CGTTCGG351.2107375E-745.045
ACGATAT207.030804E-445.038
TATCTCA207.030804E-445.010
GTCACGA207.030804E-445.011
CATACGA700.045.018
TTAGTCG207.030804E-445.01
ACGTAAG207.030804E-445.01
TAGTCGT207.030804E-445.015
AGTCGGC406.8066583E-945.030
CTTACCG207.030804E-445.033
ACGGGTA302.1639007E-644.9999965