##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546972_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	623934
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570936990130367	31.0	31.0	33.0	30.0	34.0
2	31.94525542765741	31.0	31.0	34.0	30.0	34.0
3	32.0536963845535	33.0	31.0	34.0	30.0	34.0
4	35.730780819766196	37.0	35.0	37.0	33.0	37.0
5	35.6680674558527	37.0	35.0	37.0	33.0	37.0
6	35.719651437491784	37.0	35.0	37.0	33.0	37.0
7	36.05521737876121	37.0	35.0	37.0	35.0	37.0
8	35.98069507351739	37.0	35.0	37.0	35.0	37.0
9	37.747962124199034	39.0	38.0	39.0	35.0	39.0
10	37.33051252215779	39.0	37.0	39.0	34.0	39.0
11	37.02228761375402	39.0	37.0	39.0	33.0	39.0
12	36.21364439187478	38.0	35.0	39.0	32.0	39.0
13	35.87073472514721	38.0	35.0	39.0	31.0	39.0
14	36.79532450547654	39.0	35.0	41.0	31.0	41.0
15	37.038439963201235	39.0	35.0	41.0	32.0	41.0
16	37.18537697897534	39.0	35.0	41.0	32.0	41.0
17	37.16787993601887	39.0	35.0	41.0	32.0	41.0
18	37.1154192590883	38.0	35.0	41.0	32.0	41.0
19	37.03006087182298	38.0	35.0	41.0	32.0	41.0
20	36.85659380639619	38.0	35.0	41.0	32.0	41.0
21	36.769082947875894	38.0	35.0	41.0	32.0	41.0
22	36.686255277000456	38.0	35.0	41.0	32.0	41.0
23	36.63052822894729	38.0	35.0	41.0	32.0	41.0
24	36.50266534601416	38.0	35.0	41.0	31.0	41.0
25	36.35949315151923	38.0	35.0	40.0	31.0	41.0
26	36.1655703968689	37.0	35.0	40.0	31.0	41.0
27	36.05833469565691	37.0	35.0	40.0	31.0	41.0
28	36.102002775934636	37.0	35.0	40.0	31.0	41.0
29	36.119241778777884	37.0	35.0	40.0	31.0	41.0
30	36.04296127475021	37.0	35.0	40.0	30.0	41.0
31	35.87822590209862	37.0	35.0	40.0	30.0	41.0
32	35.75701596643235	37.0	35.0	40.0	30.0	41.0
33	35.6182512893992	37.0	35.0	40.0	30.0	41.0
34	35.43982376341087	37.0	35.0	40.0	29.0	41.0
35	35.31159545721182	37.0	34.0	40.0	29.0	41.0
36	35.15113617786497	37.0	34.0	40.0	27.0	41.0
37	35.017456974615904	36.0	34.0	40.0	27.0	41.0
38	34.91595264883786	36.0	34.0	40.0	27.0	41.0
39	34.790771139255114	36.0	34.0	40.0	26.0	41.0
40	34.58021842053807	36.0	34.0	40.0	24.0	41.0
41	34.5119708174262	36.0	34.0	40.0	24.0	41.0
42	34.4302730737546	35.0	34.0	40.0	24.0	41.0
43	34.29645763814762	35.0	34.0	40.0	24.0	41.0
44	34.16245949090769	35.0	34.0	40.0	23.0	41.0
45	34.0429676856847	35.0	33.0	40.0	23.0	41.0
46	34.00944971743806	35.0	34.0	40.0	23.0	41.0
47	33.931707520346706	35.0	33.0	40.0	23.0	41.0
48	33.84125083742832	35.0	33.0	39.0	23.0	41.0
49	33.798286357210856	35.0	33.0	39.0	24.0	41.0
50	33.639399359547646	35.0	33.0	39.0	23.0	41.0
51	33.26507130561886	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	5.0
15	26.0
16	69.0
17	192.0
18	363.0
19	699.0
20	1161.0
21	1777.0
22	2663.0
23	3899.0
24	5425.0
25	7595.0
26	9734.0
27	11055.0
28	12078.0
29	13698.0
30	15813.0
31	18274.0
32	22884.0
33	29775.0
34	55362.0
35	94993.0
36	45298.0
37	57516.0
38	80273.0
39	133199.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.67225700154183	24.55355854946196	27.790118826670767	12.984065622325438
2	30.784345780162646	26.623649296239666	28.792788980885796	13.79921594271189
3	29.912779236265376	25.91027256087984	30.451137460051864	13.725810742802924
4	25.425125093359235	29.091217981389057	29.883449210974238	15.600207714277472
5	23.908778813143698	32.60216625476413	28.90145432048903	14.587600611603149
6	21.87571121304497	41.59734843749499	25.43714559552773	11.089794753932306
7	86.76494629239632	4.278817951898758	6.985354220157901	1.970881535547029
8	86.29758916808508	3.5819493728503335	6.501969759622011	3.6184916994425693
9	81.066587171079	5.328287927889809	9.918837569358297	3.6862873316729017
10	52.92755323479727	20.538230005096693	13.96221395211673	12.572002807989307
11	44.004494065077395	21.35209813858517	20.013975837187907	14.629431959149525
12	38.05386467158385	19.489721669279124	24.425660406389138	18.030753252747886
13	24.427263140011604	32.20917597053535	26.75715700699112	16.606403882461926
14	19.525462629060126	34.67177618145509	28.351556414620777	17.451204774864006
15	17.69690383918812	22.165966272073646	42.84443546913616	17.29269441960207
16	21.277250478415986	18.60517298303987	40.5140607820699	19.603515756474245
17	21.86561399122343	19.792798597287533	26.96919866524344	31.372388746245594
18	23.981863466328168	22.096888452945343	31.645494555513885	22.2757535252126
19	29.060766042562193	24.43463571467495	24.982770613558486	21.52182762920437
20	30.050454054435242	23.743536976667404	23.392538313347245	22.813470655550105
21	25.21308343510692	26.965352104549517	26.8563662182218	20.96519824212176
22	24.87827238137367	23.086897011542888	25.68861450089272	26.346216106190717
23	23.04763003779246	27.489445999096056	23.848355755576712	25.61456820753477
24	23.738728775800006	23.089461385338833	33.093243836687854	20.078566002173307
25	23.01493427189414	22.809944641580678	30.210086323232904	23.96503476329227
26	20.80043722573221	28.504296928841832	26.689521648123037	24.00574419730292
27	21.74364596255373	27.761590168190864	27.21730183000125	23.27746203925415
28	19.605759583545694	26.928168684508297	31.737651738805706	21.7284199931403
29	21.158808463715715	23.96038683578712	30.840601730311217	24.040202970185952
30	23.833931152974515	26.01044341228399	28.145765417496083	22.009860017245416
31	28.717781047354368	24.55067362894152	23.801555933800692	22.92998938990342
32	30.93003426644485	24.734827722162922	24.224677610131838	20.11046040126039
33	28.957710270637598	24.513810755624792	23.46834761368991	23.0601313600477
34	23.383563005061433	25.903220532940985	27.064881862504684	23.648334599492895
35	22.72163401898278	25.280718793975005	28.75881102808951	23.23883615895271
36	30.968980693470783	23.565152724486886	25.18567669016274	20.28018989187959
37	23.979299092532223	28.63764436623104	26.53020992604987	20.852846615186863
38	24.885164135950276	27.73931217083858	24.275323992601784	23.10019970060936
39	24.06921244875259	26.89258799808954	25.740543070260635	23.29765648289723
40	26.900601666201872	22.68044376488539	26.03336250308526	24.385592065827474
41	20.54015328544365	21.987742293255376	27.115688518336878	30.356415902964095
42	24.63449659739652	23.718053512070185	25.395314247981354	26.252135642551938
43	23.219282808758617	23.52668711754769	27.90247045360567	25.35155962008802
44	22.901140184699024	25.905304086650187	26.805719835751855	24.38783589289893
45	21.413643109687882	28.367583750845444	25.151538464004208	25.06723467546247
46	24.458195898925204	26.52027297759058	26.419941852824174	22.60158927066004
47	23.9039706122763	24.10751778232954	27.568140219959165	24.420371385434997
48	24.835479393653817	21.725535072619863	29.514019110995715	23.92496642273061
49	24.713992185070857	20.469312459330634	29.676536300313817	25.140159055284695
50	21.903117957989146	26.222004250449565	27.404661390467584	24.470216401093705
51	20.983469405417882	27.608368833883073	25.367266409588197	26.040895351110855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	475.0
1	712.5
2	950.0
3	2871.5
4	4793.0
5	3469.5
6	2146.0
7	2309.5
8	2473.0
9	2765.5
10	3058.0
11	3200.0
12	3342.0
13	3398.0
14	3454.0
15	3325.5
16	3197.0
17	3066.0
18	2935.0
19	2848.5
20	2762.0
21	2650.0
22	2538.0
23	2396.5
24	2255.0
25	2288.0
26	2801.0
27	3281.0
28	3387.5
29	3494.0
30	4264.0
31	5034.0
32	5777.5
33	6521.0
34	7488.0
35	8455.0
36	9425.5
37	10396.0
38	13254.5
39	16113.0
40	21883.5
41	27654.0
42	34329.5
43	41005.0
44	46781.0
45	52557.0
46	54466.0
47	56375.0
48	52279.5
49	48184.0
50	46137.5
51	44091.0
52	40972.5
53	37854.0
54	35732.0
55	33610.0
56	32810.5
57	32011.0
58	32023.5
59	32036.0
60	29802.5
61	27569.0
62	26397.5
63	25226.0
64	23512.5
65	21799.0
66	18955.0
67	16111.0
68	13982.5
69	11854.0
70	10084.5
71	8315.0
72	7642.0
73	6969.0
74	5386.5
75	3317.5
76	2831.0
77	2418.0
78	2005.0
79	1534.5
80	1064.0
81	788.5
82	513.0
83	414.0
84	315.0
85	224.0
86	133.0
87	88.5
88	44.0
89	26.5
90	9.0
91	5.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.408068750205828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.18238132884296	20.646457011868932
2	6.19897268291847	3.2740579358231754
3	2.3734886271862274	1.8803775253369661
4	1.3694264274177324	1.4465562897438489
5	0.9612177292158818	1.2691951938524868
6	0.7090305425319668	1.1234476387907948
7	0.6199439698883307	1.1460066084660596
8	0.4895078133300877	1.0341564790547102
9	0.4386723924057351	1.0426041627721128
>10	7.927137215259872	53.58172539046407
>50	0.6941685053214315	10.938960069978014
>100	0.030553191255344694	1.2727264128151974
>500	0.004888510600855151	0.8810810465285885
>1k	6.110638251068938E-4	0.46264823450502984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2867	0.45950372956113944	No Hit
GAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	870	0.13943782515458367	Illumina PCR Primer Index 5 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	858	0.13751454480762387	No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	707	0.11331326710838005	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	650	0.10417768546032112	No Hit
AAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	636	0.10193385838886805	Illumina PCR Primer Index 5 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	630	0.10097221821538815	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08782980251116304	0.0
2	0.0	0.0	0.0	0.27727291668670084	0.0
3	0.0	0.0	0.0	0.42488468331586354	0.0
4	0.0	0.0	0.0	0.864835062682912	0.0
5	0.0	0.0	0.0	1.3815563825661048	0.0
6	0.0	0.0	0.0	2.4100305481028443	0.0
7	0.0	0.0	0.0	2.832511130985008	0.0
8	0.0	0.0	0.0	4.1167815826673975	0.0
9	0.0	0.0	0.0	4.638150830055743	0.0
10	0.0	0.0	0.0	5.448172402850301	0.0
11	0.0	0.0	0.0	6.121801344372963	0.0
12	0.0	0.0	0.0	6.673622530588172	0.0
13	0.0	0.0	0.0	6.9244503425041755	0.0
14	0.0	0.0	0.0	7.031192401760443	0.0
15	0.0	0.0	0.0	7.153160430430142	0.0
16	0.0	0.0	0.0	7.395814300871566	0.0
17	0.0	0.0	0.0	7.645039379165104	0.0
18	0.0	0.0	0.0	8.032259822353005	0.0
19	0.0	0.0	0.0	8.1975016588293	0.0
20	0.0	0.0	0.0	8.381174931963958	0.0
21	0.0	0.0	0.0	8.603634358762305	0.0
22	0.0	0.0	0.0	8.825452692111666	0.0
23	0.0	0.0	0.0	9.067465469104103	0.0
24	0.0	0.0	0.0	9.254183936121448	0.0
25	0.0	0.0	0.0	9.401955976112857	0.0
26	0.0	0.0	0.0	9.55918414447682	0.0
27	0.0	0.0	0.0	9.706795911105983	0.0
28	0.0	0.0	0.0	9.857292598255585	0.0
29	0.0	0.0	0.0	10.025098808527826	0.0
30	0.0	0.0	0.0	10.26486775844881	0.0
31	0.0	0.0	0.0	10.452227318915142	0.0
32	0.0	0.0	0.0	10.623238996432315	0.0
33	0.0	0.0	0.0	10.80466844249552	0.0
34	0.0	0.0	0.0	11.001964951421144	0.0
35	0.0	0.0	0.0	11.245740735398295	0.0
36	0.0	0.0	0.0	11.422201707231855	0.0
37	0.0	0.0	0.0	11.617414662448272	0.0
38	0.0	0.0	0.0	11.865998647292823	0.0
39	0.0	0.0	0.0	12.256104011001163	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	25	3.8887476E-5	45.000004	28
CTAAGCG	25	3.8887476E-5	45.000004	1
CGGATTA	25	3.8887476E-5	45.000004	40
CGGTAGT	25	3.8887476E-5	45.000004	12
ATACCGA	25	3.8887476E-5	45.000004	37
CCCGACA	25	3.8887476E-5	45.000004	26
TACCCGT	20	7.030804E-4	45.0	36
CACGGTA	20	7.030804E-4	45.0	36
ATCACGA	20	7.030804E-4	45.0	35
CGTTCGG	35	1.2107375E-7	45.0	45
ACGATAT	20	7.030804E-4	45.0	38
TATCTCA	20	7.030804E-4	45.0	10
GTCACGA	20	7.030804E-4	45.0	11
CATACGA	70	0.0	45.0	18
TTAGTCG	20	7.030804E-4	45.0	1
ACGTAAG	20	7.030804E-4	45.0	1
TAGTCGT	20	7.030804E-4	45.0	15
AGTCGGC	40	6.8066583E-9	45.0	30
CTTACCG	20	7.030804E-4	45.0	33
ACGGGTA	30	2.1639007E-6	44.999996	5
>>END_MODULE