FastQCFastQC Report
Thu 26 May 2016
SRR1546971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546971_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1984505
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161450.8135530018820815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95500.041.630891
TCGTTAG1100.040.9090921
TCGATAG1000.040.51
TATAGCG800.039.3750041
AACACGT7600.039.37541
AGGGCGA26200.039.3320666
GACACGA9500.039.3157925
CACGACC9500.039.3157927
CGTAAGG2950.038.8983042
GCTACGA1400.038.57142610
GGCGATA10050.038.507468
ACGACCA9700.038.50515728
GGGCGAT49100.038.447057
GACCGAT13300.038.2330869
TAGGGAT23600.037.754245
ACACGAC9900.037.72727226
ACACGTG7800.037.542
GCGTAAG1800.037.51
TACGGGA5350.037.4299054
GTTGATC9200.037.17391216
Produced by FastQC (version 0.11.5)