FastQCFastQC Report
Thu 26 May 2016
SRR1546970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546970_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1506349
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267391.7750866499064957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT136250.043.8770641
TATCGCG501.0822987E-940.51
ACACGAC6150.039.87804826
CACGACC6300.039.28571327
GCGAGAC6350.038.9763821
TACGCGG700.038.571432
GGGCGAT31400.038.4792987
TTCGTAG950.037.8947371
AGGGCGA16150.037.8947376
TAGGGAT21950.037.619595
CGCCGTT301.1400903E-437.49999626
CGCAACC301.1400903E-437.49999626
GTTTTTT163350.037.3002782
ACTAATC7250.037.2413810
ACGACCA6650.037.21804428
AGGGATT26300.037.214836
ATAGGGA19750.037.139244
TAGGGAC15400.036.9642875
AGGGATC15950.036.9592486
GCGCGAC4750.036.947379
Produced by FastQC (version 0.11.5)