##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546970_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1506349
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.670958058192358	31.0	31.0	33.0	30.0	34.0
2	32.05484651963124	33.0	31.0	34.0	30.0	34.0
3	32.01904140408365	33.0	31.0	34.0	30.0	34.0
4	35.784866588021764	37.0	35.0	37.0	35.0	37.0
5	35.770490769403374	37.0	35.0	37.0	35.0	37.0
6	35.837989071589654	37.0	35.0	37.0	35.0	37.0
7	36.17588951829888	37.0	35.0	37.0	35.0	37.0
8	36.12771276775833	37.0	35.0	37.0	35.0	37.0
9	37.89145742454106	39.0	38.0	39.0	35.0	39.0
10	37.45480960919415	39.0	37.0	39.0	35.0	39.0
11	37.2551334385325	39.0	37.0	39.0	34.0	39.0
12	37.07996885183978	39.0	37.0	39.0	33.0	39.0
13	37.03808878287834	39.0	37.0	39.0	33.0	39.0
14	38.21615774299316	40.0	37.0	41.0	33.0	41.0
15	38.27155459989684	40.0	37.0	41.0	33.0	41.0
16	38.30592777636524	40.0	37.0	41.0	33.0	41.0
17	38.25351761112464	40.0	37.0	41.0	33.0	41.0
18	38.07360312915533	40.0	37.0	41.0	33.0	41.0
19	37.93231648177149	40.0	37.0	41.0	33.0	41.0
20	37.70976778953616	40.0	35.0	41.0	33.0	41.0
21	37.65659618056639	39.0	35.0	41.0	33.0	41.0
22	37.584896328805605	39.0	35.0	41.0	33.0	41.0
23	37.488412711795206	39.0	35.0	41.0	33.0	41.0
24	37.398254322205545	39.0	35.0	41.0	33.0	41.0
25	37.28723556094902	39.0	35.0	41.0	33.0	41.0
26	37.16300339429973	39.0	35.0	41.0	32.0	41.0
27	37.08148310915996	39.0	35.0	41.0	32.0	41.0
28	37.07380494161711	39.0	35.0	41.0	32.0	41.0
29	37.036299024993546	39.0	35.0	41.0	32.0	41.0
30	36.93638658770312	39.0	35.0	41.0	32.0	41.0
31	36.773348008993935	39.0	35.0	41.0	31.0	41.0
32	36.531273961080736	39.0	35.0	41.0	31.0	41.0
33	36.216502948519896	39.0	35.0	41.0	30.0	41.0
34	35.86736075106101	39.0	35.0	41.0	27.0	41.0
35	35.614557449834	39.0	35.0	41.0	25.0	41.0
36	35.46996545953162	39.0	35.0	41.0	25.0	41.0
37	35.38382207576066	39.0	35.0	41.0	24.0	41.0
38	35.284829080113575	39.0	35.0	41.0	24.0	41.0
39	35.18036524072443	39.0	35.0	41.0	23.0	41.0
40	35.073468366228546	38.0	35.0	41.0	23.0	41.0
41	34.989400862615504	38.0	35.0	41.0	23.0	41.0
42	34.8971413663102	38.0	35.0	41.0	23.0	41.0
43	34.77754756699809	38.0	34.0	41.0	22.0	41.0
44	34.615368682821845	38.0	34.0	40.0	21.0	41.0
45	34.51191656116876	38.0	34.0	40.0	22.0	41.0
46	34.471177662015904	38.0	34.0	40.0	22.0	41.0
47	34.42481523206109	38.0	34.0	40.0	22.0	41.0
48	34.31163428926497	37.0	34.0	40.0	21.0	41.0
49	34.220156152392306	37.0	34.0	40.0	20.0	41.0
50	34.12687697206955	37.0	34.0	40.0	20.0	41.0
51	33.781276450543665	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	3.0
14	14.0
15	30.0
16	80.0
17	189.0
18	505.0
19	913.0
20	1771.0
21	2947.0
22	4532.0
23	7263.0
24	11643.0
25	19577.0
26	29060.0
27	33221.0
28	32020.0
29	30828.0
30	31921.0
31	36267.0
32	44084.0
33	55214.0
34	85986.0
35	127387.0
36	115995.0
37	146823.0
38	222916.0
39	464826.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.839400431108594	23.30535619567577	26.84112380331517	19.014119569900465
2	36.407897505823684	24.296295214455615	26.846169114859837	12.449638164860865
3	27.341804588445306	24.249028611563457	35.67685841727249	12.732308382718745
4	23.33151215289418	27.343663387435445	35.15128300281011	14.173541456860264
5	20.977675160271623	30.881688108134302	34.02000466027461	14.120632071319461
6	18.927419874146032	39.77239006365723	31.247539580800996	10.052650481395746
7	83.60061313812403	2.799085736439564	12.079006923362382	1.5212942020740214
8	83.44361100913534	2.3353817740775877	11.893923652486906	2.327083564300172
9	77.67144267364336	4.810837329197948	14.273850216649661	3.243869780509032
10	34.201370333169805	32.10869459866206	20.322050202177582	13.36788486599055
11	24.829106667843906	25.07991176015651	32.965136233369556	17.125845338630025
12	24.09448275266887	22.176467737556173	34.71333668359723	19.015712826177733
13	22.34309578988667	24.379277312229767	35.98110398055165	17.296522917331906
14	19.461094341351174	27.289426288330258	34.93267496443387	18.316804405884692
15	18.414988823971072	24.68153130516235	38.994283529248534	17.909196341618046
16	21.258021879391826	23.338017949359678	37.3781241929991	18.025835978249397
17	20.73257923628588	23.385284552251836	33.94804258508486	21.93409362637742
18	21.589817499132007	23.371409945503995	35.49131044664948	19.547462108714512
19	23.20119706654965	25.798072027133156	32.09906867532026	18.901662230996934
20	24.233494362860135	25.537906554191625	31.256899961429923	18.97169912151832
21	23.498671290650442	25.714824386646125	32.87325845471402	17.913245867989424
22	22.590183284218995	23.119144368270568	32.63413724176801	21.656535105742428
23	20.317535976058668	26.535351369436967	31.99052809143167	21.1565845630727
24	20.12521666625729	25.558618885796054	35.073213445224184	19.242951002722478
25	21.62712625029127	25.779815965622845	32.914417575209995	19.678640208875898
26	19.751863611951812	27.16375819946108	32.26888324020529	20.815494948381815
27	19.880319899306205	25.556361772736597	33.347716896947524	21.215601431009677
28	19.690788787990034	25.959123682493235	34.75210591967731	19.597981609839422
29	20.31036632281098	24.923507102271785	33.99789822942758	20.76822834548966
30	21.840091506018858	24.914545035712177	34.017017304754745	19.228346153514224
31	23.646246653332	25.10235011939464	31.554706113921803	19.696697113351554
32	23.66237837313929	25.162827472252445	31.55510442799112	19.619689726617136
33	23.13959115716212	25.21221841684762	31.161171813437655	20.487018612552603
34	22.24245510170618	25.777160538494066	31.830870535314194	20.14951382448556
35	22.04509048036013	26.136307057660606	31.461699778736534	20.35690268324273
36	24.495585020470024	26.052130017678504	29.51659940691035	19.935685554941116
37	22.682924076691393	27.19230404109539	30.223872422659028	19.900899459554193
38	22.613816585665074	27.37068235847071	29.660191628898747	20.355309426965466
39	21.985608912675612	26.82877606716637	29.607282243357947	21.578332776800064
40	22.910361410270795	25.92227963108151	30.555535271042768	20.61182368760493
41	20.634925903625255	25.89240607588281	31.061194982039353	22.411473038452577
42	22.02417899172104	27.116159668177826	29.598121019763678	21.261540320337453
43	21.638345429910334	26.12555257778908	30.700521592273773	21.53558040002682
44	21.98507782724986	27.179624376555502	30.190878740584022	20.64441905561062
45	20.800027085356714	27.307549578484135	29.825558353343084	22.066864982816067
46	22.62895252029908	27.05986461304784	29.36862573015948	20.9425571364936
47	21.751798553987157	26.706958347633915	30.48337403881836	21.057869059560566
48	21.56306407080962	26.591248110497634	30.42973441081715	21.415953407875598
49	22.006852329705797	25.885966665095538	30.34489351405285	21.76228749114581
50	21.1077910895815	26.323912984308418	31.20571660352282	21.362579322587262
51	20.951120888983894	26.85652528066205	30.198247550866363	21.99410627948769
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	717.0
1	1261.5
2	1806.0
3	21295.5
4	40785.0
5	27451.0
6	14117.0
7	13758.5
8	13400.0
9	14032.0
10	14664.0
11	14872.5
12	15081.0
13	15068.5
14	15056.0
15	14524.5
16	13993.0
17	13236.0
18	12479.0
19	11930.0
20	11381.0
21	11395.0
22	11409.0
23	11296.5
24	11184.0
25	12363.0
26	15397.5
27	17253.0
28	18841.5
29	20430.0
30	23286.0
31	26142.0
32	29344.0
33	32546.0
34	37429.0
35	42312.0
36	45926.5
37	49541.0
38	54350.5
39	59160.0
40	64786.5
41	70413.0
42	76440.0
43	82467.0
44	86833.5
45	91200.0
46	95487.0
47	99774.0
48	108071.0
49	116368.0
50	119093.0
51	121818.0
52	115716.0
53	109614.0
54	96711.0
55	83808.0
56	75548.5
57	67289.0
58	61356.0
59	55423.0
60	50868.0
61	46313.0
62	41427.0
63	36541.0
64	32161.0
65	27781.0
66	23570.5
67	19360.0
68	16495.5
69	13631.0
70	11836.5
71	10042.0
72	8595.0
73	7148.0
74	6214.5
75	3944.0
76	2607.0
77	1901.0
78	1195.0
79	969.0
80	743.0
81	529.0
82	315.0
83	219.0
84	123.0
85	78.5
86	34.0
87	32.0
88	30.0
89	22.5
90	15.0
91	12.0
92	9.0
93	8.5
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1506349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.53518960338645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59019310239589	23.942708855362717
2	7.553537665758195	4.9151156026352805
3	3.4509529840612583	3.3683222894641593
4	2.188111477232854	2.847624871404677
5	1.666940206492993	2.711710783787884
6	1.2890642408850086	2.5163969692883508
7	1.0517103972851063	2.3952318028466677
8	0.8951804409874706	2.3299892301416363
9	0.7957045625392157	2.3299558929443753
>10	7.37904686058717	46.51178254960673
>50	0.11488615627519916	2.33316521583138
>100	0.02158792085275144	1.3387778305316436
>500	0.002878385670450397	0.670592468561598
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.055989764607426E-4	1.7886256375928746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26739	1.7750866499064957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9915703465797104E-4	0.0	0.0	0.07229400358084348	0.0
2	1.9915703465797104E-4	0.0	0.0	0.20566283112346476	0.0
3	1.9915703465797104E-4	0.0	0.0	0.35343735083967925	0.0
4	1.9915703465797104E-4	0.0	0.0	0.5148873202690745	0.0
5	1.9915703465797104E-4	0.0	0.0	0.8241781950929035	0.0
6	1.9915703465797104E-4	0.0	0.0	1.5680297195404251	0.0
7	1.9915703465797104E-4	0.0	0.0	1.9758369408417307	0.0
8	1.9915703465797104E-4	0.0	0.0	2.976866582710912	0.0
9	1.9915703465797104E-4	0.0	0.0	3.606733897655855	0.0
10	1.9915703465797104E-4	0.0	0.0	4.490194503398615	0.0
11	1.9915703465797104E-4	0.0	0.0	5.072994372486058	0.0
12	1.9915703465797104E-4	0.0	0.0	5.569227317175502	0.0
13	1.9915703465797104E-4	0.0	0.0	5.885820616603456	0.0
14	1.9915703465797104E-4	0.0	0.0	6.0785382404741535	0.0
15	1.9915703465797104E-4	0.0	0.0	6.229432887066676	0.0
16	1.9915703465797104E-4	0.0	0.0	6.44107042922988	0.0
17	1.9915703465797104E-4	0.0	0.0	6.677204286656014	0.0
18	1.9915703465797104E-4	0.0	0.0	7.006742793336737	0.0
19	1.9915703465797104E-4	0.0	0.0	7.188374008944806	0.0
20	1.9915703465797104E-4	0.0	0.0	7.392443583791008	0.0
21	1.9915703465797104E-4	0.0	0.0	7.6058071535879135	0.0
22	1.9915703465797104E-4	0.0	0.0	7.838223413033766	0.0
23	1.9915703465797104E-4	0.0	0.0	8.102239255312016	0.0
24	1.9915703465797104E-4	0.0	0.0	8.3158683678218	0.0
25	1.9915703465797104E-4	0.0	0.0	8.49650379825658	0.0
26	1.9915703465797104E-4	0.0	0.0	8.673488016389296	0.0
27	1.9915703465797104E-4	0.0	0.0	8.870919023413565	0.0
28	1.9915703465797104E-4	0.0	0.0	9.058657721417813	0.0
29	1.9915703465797104E-4	0.0	0.0	9.264652480932373	0.0
30	1.9915703465797104E-4	0.0	0.0	9.572748413548254	0.0
31	1.9915703465797104E-4	0.0	0.0	9.790692595142295	0.0
32	1.9915703465797104E-4	0.0	0.0	10.016005587018679	0.0
33	1.9915703465797104E-4	0.0	0.0	10.227709514860102	0.0
34	1.9915703465797104E-4	0.0	0.0	10.451296479102785	0.0
35	1.9915703465797104E-4	0.0	0.0	10.700707472172784	0.0
36	1.9915703465797104E-4	0.0	0.0	10.917523097237094	0.0
37	1.9915703465797104E-4	0.0	0.0	11.145823444633349	0.0
38	1.9915703465797104E-4	0.0	0.0	11.396694922624173	0.0
39	1.9915703465797104E-4	0.0	0.0	11.730349341354493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13625	0.0	43.877064	1
TATCGCG	50	1.0822987E-9	40.5	1
ACACGAC	615	0.0	39.878048	26
CACGACC	630	0.0	39.285713	27
GCGAGAC	635	0.0	38.97638	21
TACGCGG	70	0.0	38.57143	2
GGGCGAT	3140	0.0	38.479298	7
TTCGTAG	95	0.0	37.894737	1
AGGGCGA	1615	0.0	37.894737	6
TAGGGAT	2195	0.0	37.61959	5
CGCCGTT	30	1.1400903E-4	37.499996	26
CGCAACC	30	1.1400903E-4	37.499996	26
GTTTTTT	16335	0.0	37.300278	2
ACTAATC	725	0.0	37.24138	10
ACGACCA	665	0.0	37.218044	28
AGGGATT	2630	0.0	37.21483	6
ATAGGGA	1975	0.0	37.13924	4
TAGGGAC	1540	0.0	36.964287	5
AGGGATC	1595	0.0	36.959248	6
GCGCGAC	475	0.0	36.94737	9
>>END_MODULE