FastQCFastQC Report
Thu 26 May 2016
SRR1546969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546969_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2031742
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111980.5511526561935521No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTT23960.11792835901408742No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG207.034568E-445.039
CGTAGAA207.034568E-445.020
TCGATAG1150.041.086961
CGTTTTT70200.040.961541
TTACGGG5550.038.918923
ATATGCT13300.038.57142634
TAGCATA13450.038.3085530
TACGAAT2000.038.2512
GGGCGAT54150.038.227157
TATAGCG659.094947E-1238.0769271
TCGTAAG950.037.8947371
CATATGC13750.037.833
GTTGATC14250.037.73684316
AGGGCGA28450.037.486826
TTGATCC14400.037.187517
GGTACCT15400.036.9642878
CGTAAGG3350.036.9402962
GTAGCAT13950.036.61290429
AGCATAT13950.036.61290431
AGTACGG2650.036.5094342
Produced by FastQC (version 0.11.5)