FastQCFastQC Report
Thu 26 May 2016
SRR1546968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546968_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2185625
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199690.913651701458393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT147400.042.3592221
CGTATGG2750.040.9090922
AGGGCGA30850.040.5510526
ACGGGAT4150.040.120485
ACGTATG451.929584E-840.01
TAATACG850.039.7058831
GGCGATA12350.039.5344128
GGGCGAT65200.039.4095087
CGAATAT700.038.57142614
ACACGAC19200.038.5546926
CACGACC19100.038.52094327
TCGTTAG659.094947E-1238.076921
GCGATAT2500.037.89
TACGGGA4350.037.758624
ACGACCA19500.037.61538328
AACACGT17250.037.56521641
ATAGGGC11050.037.466064
GACACGA19700.037.4619325
TATGGGC9800.037.423474
GCGAGAC19600.037.30867421
Produced by FastQC (version 0.11.5)