FastQCFastQC Report
Thu 26 May 2016
SRR1546967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546967_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1490474
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183621.2319570821094499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG502.1827873E-1145.01
CGTTTTT124700.043.2137151
GTACGAG800.039.3751
CACGACC11650.039.2060127
CGACCAA11750.038.8723429
GCGAGAC12100.038.86363621
TATAGCG700.038.5714261
ATAGGGC7200.038.4375044
GGGCGAT36200.038.225147
AGGGCGA18850.038.196296
ACGACCA12000.038.062528
ACACGAC11950.038.03347426
GACACGA12000.037.687525
GTTTTTT148050.037.537992
TGGTCAA12600.037.514
TCAAGCG12700.037.3818917
AGACACG12400.037.37903624
GGCGATT8500.037.3235328
AACACGT11000.037.22727241
CACGTGA11000.037.02272843
Produced by FastQC (version 0.11.5)