##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546967_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1490474
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.593266974130376	31.0	31.0	33.0	30.0	34.0
2	31.969056152606488	31.0	31.0	34.0	30.0	34.0
3	31.950306412590894	33.0	31.0	34.0	30.0	34.0
4	35.717647540312676	37.0	35.0	37.0	33.0	37.0
5	35.69339753662258	37.0	35.0	37.0	33.0	37.0
6	35.754543856518126	37.0	35.0	37.0	33.0	37.0
7	36.10943498511212	37.0	35.0	37.0	35.0	37.0
8	36.04390952140057	37.0	35.0	37.0	35.0	37.0
9	37.81478308242881	39.0	38.0	39.0	35.0	39.0
10	37.323710443791704	39.0	37.0	39.0	34.0	39.0
11	37.09843177405309	39.0	37.0	39.0	33.0	39.0
12	36.80075264647354	39.0	35.0	39.0	33.0	39.0
13	36.72819116603175	39.0	35.0	39.0	33.0	39.0
14	37.84878166274621	40.0	36.0	41.0	33.0	41.0
15	37.90762066295689	40.0	36.0	41.0	33.0	41.0
16	37.95471440628954	40.0	36.0	41.0	33.0	41.0
17	37.89011415160546	40.0	36.0	41.0	33.0	41.0
18	37.727721516779226	39.0	36.0	41.0	33.0	41.0
19	37.59046518087535	39.0	36.0	41.0	33.0	41.0
20	37.37074380364904	39.0	35.0	41.0	33.0	41.0
21	37.302440700072594	39.0	35.0	41.0	32.0	41.0
22	37.22703314516053	39.0	35.0	41.0	32.0	41.0
23	37.12162305414251	39.0	35.0	41.0	32.0	41.0
24	36.99745181734133	39.0	35.0	41.0	32.0	41.0
25	36.89391965240588	38.0	35.0	41.0	32.0	41.0
26	36.75262634571284	38.0	35.0	41.0	31.0	41.0
27	36.66009068256139	38.0	35.0	41.0	31.0	41.0
28	36.645348392524795	38.0	35.0	41.0	31.0	41.0
29	36.60204673144248	38.0	35.0	41.0	31.0	41.0
30	36.47608881469922	38.0	35.0	41.0	31.0	41.0
31	36.29966171835269	38.0	35.0	41.0	30.0	41.0
32	36.05918385694752	38.0	35.0	41.0	30.0	41.0
33	35.74036044909204	38.0	35.0	41.0	28.0	41.0
34	35.3800374914289	38.0	35.0	41.0	25.0	41.0
35	35.12095615220393	38.0	35.0	41.0	24.0	41.0
36	34.97407133569589	38.0	34.0	41.0	23.0	41.0
37	34.86688060308332	38.0	34.0	41.0	23.0	41.0
38	34.745000583706926	38.0	34.0	40.0	23.0	41.0
39	34.62738363768841	38.0	34.0	40.0	22.0	41.0
40	34.507975315235285	38.0	34.0	40.0	22.0	41.0
41	34.419834227232414	38.0	34.0	40.0	21.0	41.0
42	34.32301200826046	37.0	34.0	40.0	21.0	41.0
43	34.20894426873598	37.0	34.0	40.0	20.0	41.0
44	33.991716058113056	37.0	33.0	40.0	18.0	41.0
45	33.902761135048316	37.0	33.0	40.0	20.0	41.0
46	33.85045831057771	37.0	33.0	40.0	20.0	41.0
47	33.808259654311314	36.0	33.0	40.0	20.0	41.0
48	33.701280263862365	36.0	33.0	40.0	20.0	41.0
49	33.60272571007612	36.0	33.0	40.0	20.0	41.0
50	33.501022493515485	36.0	33.0	40.0	19.0	41.0
51	33.11818455068656	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	5.0
14	17.0
15	46.0
16	121.0
17	318.0
18	759.0
19	1345.0
20	2473.0
21	3869.0
22	5956.0
23	9266.0
24	14314.0
25	22962.0
26	31971.0
27	34801.0
28	33189.0
29	32993.0
30	34868.0
31	39765.0
32	47941.0
33	60964.0
34	99042.0
35	141544.0
36	115293.0
37	148946.0
38	215046.0
39	392404.0
40	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.83331342915073	23.359615800074337	25.787098600847784	18.01997216992715
2	37.05230685003563	24.52125967980656	25.336436596679984	13.089996873477833
3	28.502610578916503	24.64128861020051	33.70088978405527	13.155211026827708
4	24.184252794748517	27.644695580063793	33.77516145870374	14.395890166483952
5	22.33832995409514	31.1464674996008	32.248331738762296	14.266870807541762
6	20.577145257146384	39.703141416757354	29.553215956803	10.166497369293257
7	83.6003177512657	2.9606688878839886	11.779608366197598	1.6594049946527079
8	83.16716695494185	2.735035968423468	11.498020092936878	2.5997769836978035
9	77.73171487728065	4.542179199368792	14.278947502606554	3.447158420744005
10	39.700927355995475	26.039568620452286	20.420014035803373	13.839489987748863
11	30.233200981701124	25.836948514365226	27.636376079019158	16.29347442491449
12	26.52726582281878	22.18260768050969	31.603637500553518	19.686488996118012
13	22.570001221088056	25.601385867851434	34.142292988673404	17.686319922387106
14	20.688720500994986	26.588655689398138	33.841650374310454	18.880973435296422
15	20.10689216987348	23.153573963718923	38.181679116844705	18.557854749562892
16	22.835956883514907	22.034265609463834	36.389095012727495	18.74068249429376
17	22.18072908349961	23.194030892185978	32.50489441613876	22.120345608175654
18	23.170078780307474	22.558259989775063	33.8362158615313	20.435445368386166
19	25.098659889404313	25.797766348155015	29.648890218816298	19.454683543624377
20	26.49861721841508	24.8299534242127	29.013857336659342	19.657572020712873
21	24.7972792547874	26.000789010744235	30.514722162211484	18.68720957225688
22	23.99484996048237	23.06407223473875	30.26889432489262	22.672183479886264
23	22.68888957472589	26.23641875000839	29.549190391781405	21.525501283484314
24	21.909406001043962	24.37647352452978	33.77610075720878	19.938019717217475
25	22.783758723734866	25.038544785081797	31.335467777364784	20.842228713818557
26	21.02492227304871	26.93170092198858	30.511233339192767	21.53214346576995
27	21.67048871701217	24.9158992374238	30.89909652902365	22.514515516540374
28	21.097181165186377	26.303176036616538	32.15232201299721	20.447320785199878
29	21.89887243923745	24.612572913046453	31.376864004336873	22.11169064337922
30	23.222813682090397	25.36568903583692	31.043077571296113	20.36841971077657
31	24.47449603280567	25.12811360681233	28.729115704131708	21.668274656250293
32	25.514567848885655	25.28410425139922	28.00115936272622	21.200168536988905
33	23.903603819992835	25.914038084528812	27.507155441825887	22.675202653652462
34	22.89412629807699	27.166793919249848	28.751122126249772	21.187957656423393
35	22.134837642253405	27.400813432505366	29.03344841976445	21.430900505476778
36	25.352941413268532	27.329762209874175	25.971536571587293	21.34575980527
37	23.3057403215353	27.64858695958467	27.97130308881604	21.074369630063995
38	23.46810477740638	27.30393150098559	27.760766038186507	21.467197683421517
39	23.282794600912194	26.26090760388977	27.67743684223945	22.77886095295859
40	24.54709038869514	25.343078778965616	28.082677054413562	22.02715377792568
41	20.97721932754278	25.35461873202753	29.024323805715497	24.64383813471419
42	23.14652922493113	26.357118607905942	27.6096731643759	22.886679002787034
43	22.257416097161038	25.246934867699807	28.870278850889047	23.625370184250112
44	22.97544271151325	26.86742606714374	27.934938818120948	22.222192403222063
45	21.54723933460094	26.765310901095894	27.57404691393476	24.113402850368406
46	23.346666899254867	26.325048273233886	27.312049723779147	23.016235103732104
47	22.259965621674716	26.482850422080496	28.89215108750639	22.365032868738403
48	22.920829212720246	25.380650719167193	28.713885649799998	22.984634418312563
49	23.098490815673404	25.07168860375961	28.604121910211116	23.225698670355875
50	21.959255914561407	26.1909969580147	29.429228554137808	22.42051857328608
51	21.71389772649506	26.33148917726844	27.8041750476694	24.1504380485671
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	1385.0
2	1954.0
3	15639.5
4	29325.0
5	20351.0
6	11377.0
7	11508.0
8	11639.0
9	12339.5
10	13040.0
11	13406.0
12	13772.0
13	13474.0
14	13176.0
15	13042.5
16	12909.0
17	12372.0
18	11835.0
19	11326.0
20	10817.0
21	10476.5
22	10136.0
23	10204.0
24	10272.0
25	10479.5
26	11602.5
27	12518.0
28	13622.0
29	14726.0
30	16972.0
31	19218.0
32	22259.5
33	25301.0
34	28324.5
35	31348.0
36	35248.5
37	39149.0
38	43802.0
39	48455.0
40	54400.5
41	60346.0
42	69216.5
43	78087.0
44	83727.0
45	89367.0
46	99607.0
47	109847.0
48	115175.0
49	120503.0
50	116998.5
51	113494.0
52	105687.0
53	97880.0
54	90763.5
55	83647.0
56	77773.0
57	71899.0
58	69372.0
59	66845.0
60	62731.5
61	58618.0
62	53307.0
63	47996.0
64	43300.5
65	38605.0
66	34048.0
67	29491.0
68	25810.0
69	22129.0
70	18495.0
71	14861.0
72	13693.5
73	12526.0
74	10623.0
75	7460.0
76	6200.0
77	4906.0
78	3612.0
79	2644.5
80	1677.0
81	1291.0
82	905.0
83	668.5
84	432.0
85	315.0
86	198.0
87	128.5
88	59.0
89	43.0
90	27.0
91	21.5
92	16.0
93	15.0
94	14.0
95	8.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1490474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.69469851051091
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32603849875564	24.627621187079534
2	7.221480710985263	4.722082692902663
3	3.175615090849257	3.1147733394223547
4	1.963437382563564	2.5677597306872957
5	1.4231732851358303	2.326511074286467
6	1.1246820017113448	2.20626833796902
7	0.9094320347009163	2.0813524333242865
8	0.7467901812901121	1.9532863862312
9	0.6719487172823915	1.977224465346509
>10	7.281590011790209	48.5145956217616
>50	0.1329789789898197	2.7481612124302663
>100	0.019927075987134307	1.2363751080713692
>500	0.0022833092531488013	0.5228362156753118
>1k	4.151471369361457E-4	0.155004921677364
>5k	0.0	0.0
>10k+	2.0757356846807284E-4	1.2461472731347452
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18362	1.2319570821094499	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.70927503599526E-5	0.0	0.0	0.06642182285635308	0.0
2	6.70927503599526E-5	0.0	0.0	0.17725904645099477	0.0
3	6.70927503599526E-5	0.0	0.0	0.30473527213490476	0.0
4	6.70927503599526E-5	0.0	0.0	0.5197004442881928	0.0
5	6.70927503599526E-5	0.0	0.0	0.8921993942866497	0.0
6	6.70927503599526E-5	0.0	0.0	1.8727599407973572	0.0
7	6.70927503599526E-5	0.0	0.0	2.3721983744768442	0.0
8	6.70927503599526E-5	0.0	0.0	3.4970083342614497	0.0
9	6.70927503599526E-5	0.0	0.0	4.177328822911369	0.0
10	6.70927503599526E-5	0.0	0.0	5.05376142086343	0.0
11	6.70927503599526E-5	0.0	0.0	5.66262813038	0.0
12	6.70927503599526E-5	0.0	0.0	6.142072924452221	0.0
13	6.70927503599526E-5	0.0	0.0	6.472638905475708	0.0
14	6.70927503599526E-5	0.0	0.0	6.661773368740414	0.0
15	6.70927503599526E-5	0.0	0.0	6.819441332086303	0.0
16	6.70927503599526E-5	0.0	0.0	7.0412499647763065	0.0
17	6.70927503599526E-5	0.0	0.0	7.262521855463429	0.0
18	6.70927503599526E-5	0.0	0.0	7.57336256788109	0.0
19	6.70927503599526E-5	0.0	0.0	7.766455503417034	0.0
20	6.70927503599526E-5	0.0	0.0	7.959950995455137	0.0
21	6.70927503599526E-5	0.0	0.0	8.178203712376062	0.0
22	6.70927503599526E-5	0.0	0.0	8.420878190428011	0.0
23	6.70927503599526E-5	0.0	0.0	8.706693306961409	0.0
24	6.70927503599526E-5	0.0	0.0	8.950307083518398	0.0
25	6.70927503599526E-5	0.0	0.0	9.162454360156568	0.0
26	6.70927503599526E-5	0.0	0.0	9.35662077969827	0.0
27	6.70927503599526E-5	0.0	0.0	9.559307978535687	0.0
28	6.70927503599526E-5	0.0	0.0	9.772595831929976	0.0
29	6.70927503599526E-5	0.0	0.0	10.00896359144809	0.0
30	6.70927503599526E-5	0.0	0.0	10.314772347588754	0.0
31	6.70927503599526E-5	0.0	0.0	10.556641712636383	0.0
32	6.70927503599526E-5	0.0	0.0	10.84440251893022	0.0
33	6.70927503599526E-5	0.0	0.0	11.08419200871669	0.0
34	6.70927503599526E-5	0.0	0.0	11.332099721296714	0.0
35	6.70927503599526E-5	0.0	0.0	11.59396272595161	0.0
36	1.341855007199052E-4	0.0	0.0	11.824963065440928	0.0
37	2.0127825107985783E-4	0.0	0.0	12.087832461351221	0.0
38	2.0127825107985783E-4	0.0	0.0	12.374318505388219	0.0
39	2.0127825107985783E-4	0.0	0.0	12.749635350901794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	50	2.1827873E-11	45.0	1
CGTTTTT	12470	0.0	43.213715	1
GTACGAG	80	0.0	39.375	1
CACGACC	1165	0.0	39.20601	27
CGACCAA	1175	0.0	38.87234	29
GCGAGAC	1210	0.0	38.863636	21
TATAGCG	70	0.0	38.571426	1
ATAGGGC	720	0.0	38.437504	4
GGGCGAT	3620	0.0	38.22514	7
AGGGCGA	1885	0.0	38.19629	6
ACGACCA	1200	0.0	38.0625	28
ACACGAC	1195	0.0	38.033474	26
GACACGA	1200	0.0	37.6875	25
GTTTTTT	14805	0.0	37.53799	2
TGGTCAA	1260	0.0	37.5	14
TCAAGCG	1270	0.0	37.38189	17
AGACACG	1240	0.0	37.379036	24
GGCGATT	850	0.0	37.323532	8
AACACGT	1100	0.0	37.227272	41
CACGTGA	1100	0.0	37.022728	43
>>END_MODULE