FastQCFastQC Report
Thu 26 May 2016
SRR1546966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546966_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2859705
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT653302.2845013733934096No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT80290.2807632255774634No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC64740.22638698746898717No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG55810.19515999027871755No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT48860.17085678417878766No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC33430.11690016977275629TruSeq Adapter, Index 14 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT32870.11494192582801374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT302.1666401E-645.00000440
TACGACC253.892267E-545.028
CGTTTTT387300.044.378391
TGTTACG900.042.51
TAATGCG501.0822987E-940.51
CGACGGT950.040.26315728
GTCGACG850.039.7058831
TACGACG403.4606273E-739.3751
GCGTAAG2000.039.3751
GTTTTTT437950.039.3332562
CGGTTTT11000.038.4545441
GTTAGCG1350.038.3333321
TACGGGT5400.038.3333324
CGTTAGG3700.038.310812
AGGGCGA29600.037.9307446
GGACCGA15500.037.8870968
GGGACCG16900.037.8106547
CGTTCTG6850.037.773721
ATTAGCG1200.037.5000041
TATTACG1200.037.5000041
Produced by FastQC (version 0.11.5)